2.7.7.80 | MoeB-MoaD complex in its apo, ATP-bound, and MoaD-adenylate forms, equal volumes of 23 mg/ml MoeB and 10 mg/ml MoaD at 4°C for 1 h, followed by hanging drop vapour diffusion against a reservoir containing 1.7 M Li2SO4, and 100 mM HEPES, pH 7.5, for the ternary complex, crystals of the apo complex are soaked for 24 h in a solution consisting of 1.7 M Li2SO4, 100 mM HEPES, pH 7.5, and 20 mM ATP, X-ray diffraction structure determination and analysis at 1.7-3.5 A resolutions, modeling |
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