EC Number |
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2.7.11.7 | alpha-kinase domain in complex with AMP and adenosine, hanging drop vapor diffusion method, using 200 mM NaH2PO4, 20% (w/v) polyethylene glycol 8000, and 100 mM Tris-HCl, pH 7.5 |
2.7.11.7 | alpha-kinase domain of myosin-II heavy chain kinase A, hanging drop vapor diffusion method, using 5% PEG (w/v) 8000, 20% (w/v) PEG 300, 10% (v/v) glycerol and 0.1 M Tris-HCl, pH 9.0 |
2.7.11.7 | MHCK A in complex with ATP and a peptide substrate, and MHCK A alpha kinase domain in individual complexes with AMP, ADP, and beta,gamma-methyleneadenosine 5'-triphosphate, X-ray diffraction structcure determination and analysis |
2.7.11.7 | purified recombinant wild-type and truncation mutant enzymes, hanging drop vapor diffusion method, mixing of 0.001 ml protein solution with 0.001 ml of reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 0.2 M NaH2PO4, and 18% w/v PEG 8000, 4°C, 10 days, X-ray diffraction structure determination and analysis at 1.9 A resolution |