EC Number |
Reference |
---|
2.7.11.1 | - |
491320, 491614, 762489 |
2.7.11.1 | truncation mutant of CKI delta lacking the C-terminal autoinhibitory region |
491629 |
2.7.11.1 | purified recombinant Leu-Glu-His6-tagged GSK3beta in complex with ATP or ATP analogue AMP-PNP, 10 mg/ml protein, 2 mM ATP or AMP-PNP, 12-14% w/v PEG 6000, 100 mM NaCl, 5 mM MgCl2, 10% v/v glycerol, in 100 mM HEPES-NaOH, pH 7.5, hanging drop vapor diffusion method, 4°C, several days, soaking in 0.1 mM ethylmercuric thiosalyclate at pH 7.5 for 1 h, cryoprotection by 30% w/v D-sorbitol, X-ray diffraction structure determination and analysis at 1.7-2.6 A resolution |
660602 |
2.7.11.1 | purified recombinant His-tagged N-terminal catalytic domain of PknB, residues 1-331, hanging drop vapour diffusion method, 0.001 ml protein solution containing 5 mg/ml protein mixed with equal volume of 0.1 M HEPES, pH 7.5, 30 mM MgCl2, 0.15 mM inhibitor AMP-PNP, 27% PEG 400, and 4% 1,3-butanediol, 19°C, versus 1 ml of a solution containing 0.1 M HEPES, pH 7.5, 30 mM MgCl2, and 27% PEG 400, 2-3 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution |
662128 |
2.7.11.1 | purified recombinant low activity WNK1 mutant S382A, wild-type and selenomethionine-labeled, hanging drop vapour diffusion method, 16°C, mixing of 0.002 ml of protein solution and of well solution, the latter containing 24% PEG monomethyl ester 2000, 0.3 M NaCl, 0.1 M HEPES, pH 7.0-8.0, 3 days, stabilization with 15% glycerol, X-ray diffraction structure determination and analysis at 1.8 A resolution |
663401 |
2.7.11.1 | purified recombinant wild-type and selenomethionine-labeled Rio2, hanging drop vapour diffusion method, equal volumes of protein solution, containing 5-12% PEG 900, and 0.1 M phosphate citrate buffer, pH 3.6-4.1, and reservoir solution, versus 1 ml reservoir solution, purified recombinant Rio2 complexed with ATP using 0.1 M Tris, pH 7.5, 20 mM ATP, 20 mM MgCl2, 20 mM adenosine 5'-(beta,gamma-imino)triphosphate, and 20% ethylene glycol, 20°C, 2-4 days, X-ray diffraction structure determination and analysis at 2.0 A resolution |
663404 |
2.7.11.1 | autoinhibitory junction region bound to the calmodulin-like domain and Ca2+, 20 mg/ml protein in 20 mM Tris buffer, pH 7.0, 10 mM CaCl2, 10 mM DTT, mixed with an equal volume of precipitating agent composed of 15% w/v PEG 400, 0.1 M calcium chloride, and 0.1 M sodium acetate at pH 7.6, equilibration against a reservoir of precipitating agent by vapour diffusion at 20°C, X-ray diffraction structure determination and anaylsis at 2.0 A resolution, multiple-wavelength anomalous dispersion |
675373 |
2.7.11.1 | hanging drop vapor diffusion in 1 ml wells containing 512% poly(ethylene glycol) 900 and 100 mM sodium phosphate/citrate buffer, pH 3.64.1. Crystallization of the enzyme after incubation with ATP or ADP and Mn2+. Co-crystal structures of Rio2ATPMn and Rio2ADPMn are solved at 1.84 and 1.75 A resolution, respectively |
722223 |
2.7.11.1 | hanging drop vapor diffusion method, high resolution crystal structures of Archaeoglobus fulgidus Rio1 in the presence and absence of bound nucleotides |
722224 |
2.7.11.1 | X-ray crystal structure of toyocamycin bound to Rio1 at 2.0 A, toyocamycin binds in the ATP binding pocket of the protein |
723566 |