Literature summary for 2.7.11.1 extracted from

handran, V.; Stollar, E.J.; Lindorff-Larsen, K.; Harper, J.F.; Chazin, W.J.; Dobson, C.M.; Luisi, B.F.; Christodoulou, J.
Structure of the regulatory apparatus of a calcium-dependent protein kinase (CDPK): a novel mode of calmodulin-target recognition (2006), J. Mol. Biol., 357, 400-410.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	CDPKs contain an autoinhibitory junction region whose calcium-dependent interaction with the tethered regulatory calmodulin-like domain activates the catalytic kinase domain, activation mechanism of CDPK, overview	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
expression of autoinhibitory junction region bound to the calmodulin-like domain, residues Lys428-Gly591, in Escherichia coli	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
autoinhibitory junction region bound to the calmodulin-like domain and Ca2+, 20 mg/ml protein in 20 mM Tris buffer, pH 7.0, 10 mM CaCl2, 10 mM DTT, mixed with an equal volume of precipitating agent composed of 15% w/v PEG 400, 0.1 M calcium chloride, and 0.1 M sodium acetate at pH 7.6, equilibration against a reservoir of precipitating agent by vapour diffusion at 20°C, X-ray diffraction structure determination and anaylsis at 2.0 A resolution, multiple-wavelength anomalous dispersion	Arabidopsis thaliana

Crystallization (Comment)

Organism

autoinhibitory junction region bound to the calmodulin-like domain and Ca2+, 20 mg/ml protein in 20 mM Tris buffer, pH 7.0, 10 mM CaCl2, 10 mM DTT, mixed with an equal volume of precipitating agent composed of 15% w/v PEG 400, 0.1 M calcium chloride, and 0.1 M sodium acetate at pH 7.6, equilibration against a reservoir of precipitating agent by vapour diffusion at 20°C, X-ray diffraction structure determination and anaylsis at 2.0 A resolution, multiple-wavelength anomalous dispersion

Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	CDPKs contain an autoinhibitory junction region whose calcium-dependent interaction with the tethered regulatory calmodulin-like domain activates the catalytic kinase domain	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	dependent on	Arabidopsis thaliana
Mg2+	-	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q06850	isozyme CPK1	-

Subunits

Subunits	Comment	Organism
monomer	crystal structure, modelling of the activated monomer to account for the intra-molecular recognition of the two domains, overview	Arabidopsis thaliana
More	the autoinhibitory junction region bound to the calmodulin-like domain and Ca2+ forms symmetric dimers with domain-swap interactions, in which the J region of one protomer interacts extensively with the carboxy-terminal EF-hand domain, C-lobe, of the partner protomer	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
calcium-dependent protein kinase	-	Arabidopsis thaliana
CDPK	-	Arabidopsis thaliana
CPK1	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Arabidopsis thaliana