EC Number   |
|---|
   2.3.1.184 | crystal structure analysis, three-dimensional modeling |
| 2.3.1.184 | molecular modeling of structure |
| 2.3.1.184 | purified recombinant detagged LasIDELTAG, hanging drop vapour diffusion method, from 1.5 M ammonium sulfate, 0.125 M disodium sulfate, and 0.1 M MOPS, pH 6.5, cryoprotection with 15% glycerol, heavy atom derivatization with Hg2+, single isomorphous replacement with anomalous scattering, X-ray diffraction structure determination and analysis at 2.3-3.1 A resolution, structure modeling |
| 2.3.1.184 | purified recombinant His-tagged enzyme, vapor diffusion, 6 mg/ml protein in 0.1 M MES, pH 6.1, 14% PEG 4000, 6% 2-propanol, 0.03% 2-mercaptoethanol, 10 mM EDTA, and 0.5% NaN3, addition of agarose reduces the formation of a protein skin on the drop surface, X-ray diffraction structure determination and analysis at 1.14-1.19 A resolution, MAD phasing, modeling |
