2.1.1.265 | TehB complexed with S-adenosyl-L-methionine or sinefungin, hanging drop vapour diffusion method, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, the latter containing 0.1 M NaF and 16-18% PEG 3350, at 20°C, formation of sinefungin complex crystals by microseeding of crystals in reservoir solution containing 5 mM sinefungin, method optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution |
718496 |