Literature summary for 2.1.1.265 extracted from

Choudhury, H.G.; Beis, K.
Crystallization and initial X-ray diffraction analysis of the tellurite-resistance S-adenosyl-L-methionine transferase protein TehB from Escherichia coli (2010), Acta Crystallogr. Sect. F, 66, 1496-1499.

Search for more literature for 2.1.1.265

Cloned(Commentary)

Cloned (Comment)	Organism
gene tehB, expression as His-tagged enzyme with a TEV protease cleavage site in Escherichia coi strain BL21 (DE3) PlysS	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
TehB complexed with S-adenosyl-L-methionine or sinefungin, hanging drop vapour diffusion method, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, the latter containing 0.1 M NaF and 16-18% PEG 3350, at 20°C, formation of sinefungin complex crystals by microseeding of crystals in reservoir solution containing 5 mM sinefungin, method optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution	Escherichia coli

Crystallization (Comment)

Organism

TehB complexed with S-adenosyl-L-methionine or sinefungin, hanging drop vapour diffusion method, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, the latter containing 0.1 M NaF and 16-18% PEG 3350, at 20°C, formation of sinefungin complex crystals by microseeding of crystals in reservoir solution containing 5 mM sinefungin, method optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution

Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
sinefungin	a cofactor analogue of S-adenosyl-L-methionine	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Escherichia coli	5737	-

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	gene tehB	-
Escherichia coli MG1655	-	gene tehB	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme with a TEV protease cleavage site from Escherichia coli strain BL21(DE3) PlysS by nickel affinity chromatography, cleavage of the His-tag, followed by gel filtration	Escherichia coli

Synonyms

Synonyms	Comment	Organism
TehB	-	Escherichia coli
tellurite-resistance S-adenosyl-L-methionine transferase protein	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	TehB is a SAM-dependent methyltransferase	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	TehB is an S-adenosyl-l-methionine-dependent methyltransferase that detoxifies tellurite in bacteria, the cytoplasmic TehB is a tellurite-resistance protein	Escherichia coli