EC Number   |
Reference  |
|---|
   7.6.2.8 | purified ECF-CbrT complex in detergent (n-dodecyl-beta-D-maltopyranoside) solution, sitting drop vapour diffusion method, mixing of protein solution containing 50 mM HEPES pH 8, 150 mM NaCl, 1% polyoxyethylene(10)dodecyl ether, with precipitant solution containing 0.2 M KCl, 0.1 M sodium citrate, pH 5.5, 37% v/v pentaerythritol propoxylate, X-ray diffraction structure determination and analysis at 3.4 A resolution, molecular replacement with the structure of the folate transporter, ECF-FolT2, from Lactobacillus delbrueckii as a search model |
750412 |
| 7.6.2.8 | purified recombinant His-tagged enzyme BtuM with natively bound cobalmin and anomalously bound cobalmin, sitting drop vapour diffusion method, mixing of 0.002 ml of protein in 50mM Tris-HCl, pH 7.5, or in 50 mM HEPES-NaOH pH 8.0, 100 mM NaCl, 0.005 mM cyano-Cbl and 0.35% detergent, with 0.002 ml of precipitant solution containing 25 mM Tris, pH 8.5, and 25-30% v/v PEG 400 or 50 mM Tris, pH 8.5, and 27-30% v/v PEG 400, or 75 mM Tris, pH 8.5, and 29-30% v/v PEG 400, at 8°C, 3-4 weeks, X-ray diffraction structure determination and analysis at 2.0-2.5 A resolution, structure modeling |
751670 |
| 7.6.2.8 | purified recombinant complex of inhibitory nanobody Nb9 with enzyme BtuF complex, crystallization solution contains 100 mM Tris-HCl, pH 8.5, 400 mM MgCl2, and 33% w/v PEG4000, X-ray diffraction structrue determination and analysis at 2.7 A resolution |
752215 |
| 7.6.2.8 | analysis of the cobinamide (Cbi)-bound BtuF crystal structure model, PDB ID 5M29, crystal structures of Cbi-bound BtuF mutants W66F, W66Y and W66L, sitting drop vapor diffusion technique, mixing of 20 mg/ml protein in 10 mM Tris pH 8 and 100 mM NaCl, with precipitant solution containing 1% w/v tryptone, 50 mM HEPES, pH 7.0, and 12% w/v PEG 3350, 1-2 weeks, 20°C, X-ray diffraction structure determination and analysis at 1.5-1.7 A resolution, molecular replacement using the BtuF structure (PDB ID 1N2Z) as search model |
752221 |
