BRENDA - Enzyme Database show
show all sequences of 7.6.2.8

Functional and structural characterization of an ECF-type ABC transporter for vitamin B12

Santos, J.A.; Rempel, S.; Mous, S.T.; Pereira, C.T.; Ter Beek, J.; de Gier, J.W.; Guskov, A.; Slotboom, D.J.; eLife 7, e35828 (2018)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression of ECF-CbrT complements in an Escherichia coli strain lacking its endogenous vitamin B12 transporter (Escherichia coli strain DELTAFEC, with btuF and btuC also deleted , thus abolished endogenous Cbl-uptake, from parental strain JW0154). Recombinant ECF-CbrT supports cobalamin-dependent growth of an Escherichia coli deletion strain
Lactobacillus delbrueckii subsp. bulgaricus
Crystallization (Commentary)
Crystallization
Organism
purified ECF-CbrT complex in detergent (n-dodecyl-beta-D-maltopyranoside) solution, sitting drop vapour diffusion method, mixing of protein solution containing 50 mM HEPES pH 8, 150 mM NaCl, 1% polyoxyethylene(10)dodecyl ether, with precipitant solution containing 0.2 M KCl, 0.1 M sodium citrate, pH 5.5, 37% v/v pentaerythritol propoxylate, X-ray diffraction structure determination and analysis at 3.4 A resolution, molecular replacement with the structure of the folate transporter, ECF-FolT2, from Lactobacillus delbrueckii as a search model
Lactobacillus delbrueckii subsp. bulgaricus
Inhibitors
Inhibitors
Commentary
Organism
Structure
cobinamide
addition of a 250fold excess of cobalamin decreases the uptake of radiolabeled cobalamin to about 25%
Lactobacillus delbrueckii subsp. bulgaricus
cyano-cobalamin
a 250fold excess of unlabeled CN-Cbl inhibits the uptake of the radiolabeled substrate almost completely
Lactobacillus delbrueckii subsp. bulgaricus
hydroxycobalamin
inhibits uptake to a similar extent as cyano-cobalamin
Lactobacillus delbrueckii subsp. bulgaricus
methylcobalamin
inhibits uptake to a similar extent as cyano-cobalamin
Lactobacillus delbrueckii subsp. bulgaricus
additional information
hemin does not compete with cobalamin-uptake
Lactobacillus delbrueckii subsp. bulgaricus
S-adenosyl-cobalamin
is less effective causing about 25% inhibition of cobalmin uptake
Lactobacillus delbrueckii subsp. bulgaricus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
binding kinetics of substrates and competing ligands
Lactobacillus delbrueckii subsp. bulgaricus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
integral membrane enzyme
Lactobacillus delbrueckii subsp. bulgaricus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Lactobacillus delbrueckii subsp. bulgaricus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus delbrueckii subsp. bulgaricus
Q1GBI8
-
-
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
Q1GBI8
-
-
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
Q1GBI8
-
-
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
Q1GBI8
-
-
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
Q1GBI8
-
-
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
Q1GBI8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family
Lactobacillus delbrueckii subsp. bulgaricus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Lactobacillus delbrueckii subsp. bulgaricus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Lactobacillus delbrueckii subsp. bulgaricus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Lactobacillus delbrueckii subsp. bulgaricus
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of ECF-CbrT complements in an Escherichia coli strain lacking its endogenous vitamin B12 transporter (Escherichia coli strain DELTAFEC, with btuF and btuC also deleted , thus abolished endogenous Cbl-uptake, from parental strain JW0154). Recombinant ECF-CbrT supports cobalamin-dependent growth of an Escherichia coli deletion strain
Lactobacillus delbrueckii subsp. bulgaricus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Lactobacillus delbrueckii subsp. bulgaricus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified ECF-CbrT complex in detergent (n-dodecyl-beta-D-maltopyranoside) solution, sitting drop vapour diffusion method, mixing of protein solution containing 50 mM HEPES pH 8, 150 mM NaCl, 1% polyoxyethylene(10)dodecyl ether, with precipitant solution containing 0.2 M KCl, 0.1 M sodium citrate, pH 5.5, 37% v/v pentaerythritol propoxylate, X-ray diffraction structure determination and analysis at 3.4 A resolution, molecular replacement with the structure of the folate transporter, ECF-FolT2, from Lactobacillus delbrueckii as a search model
Lactobacillus delbrueckii subsp. bulgaricus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cobinamide
addition of a 250fold excess of cobalamin decreases the uptake of radiolabeled cobalamin to about 25%
Lactobacillus delbrueckii subsp. bulgaricus
cyano-cobalamin
a 250fold excess of unlabeled CN-Cbl inhibits the uptake of the radiolabeled substrate almost completely
Lactobacillus delbrueckii subsp. bulgaricus
hydroxycobalamin
inhibits uptake to a similar extent as cyano-cobalamin
Lactobacillus delbrueckii subsp. bulgaricus
methylcobalamin
inhibits uptake to a similar extent as cyano-cobalamin
Lactobacillus delbrueckii subsp. bulgaricus
additional information
hemin does not compete with cobalamin-uptake
Lactobacillus delbrueckii subsp. bulgaricus
S-adenosyl-cobalamin
is less effective causing about 25% inhibition of cobalmin uptake
Lactobacillus delbrueckii subsp. bulgaricus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
binding kinetics of substrates and competing ligands
Lactobacillus delbrueckii subsp. bulgaricus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
integral membrane enzyme
Lactobacillus delbrueckii subsp. bulgaricus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Lactobacillus delbrueckii subsp. bulgaricus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
?
-
-
-
-
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family
Lactobacillus delbrueckii subsp. bulgaricus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Lactobacillus delbrueckii subsp. bulgaricus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Lactobacillus delbrueckii subsp. bulgaricus
General Information
General Information
Commentary
Organism
evolution
ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family
Lactobacillus delbrueckii subsp. bulgaricus
physiological function
cobalamin-specific ECF-type ABC transporter from Lactobacillus delbrueckii, ECF-CbrT, mediates the specific, ATP-dependent uptake of cobalamin. Cobalamin (vitamin B12) is the most complex B-type vitamin and is synthetized exclusively in a limited number of prokaryotes. Its biologically active variants contain rare organometallic bonds, which are used by enzymes in a variety of central metabolic pathways such as L-methionine synthesis and ribonucleotide reduction. Enzyme ECF-CbrT catalyzes ATP-dependent transport of cobalamin and cobinamide
Lactobacillus delbrueckii subsp. bulgaricus
General Information (protein specific)
General Information
Commentary
Organism
evolution
ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family
Lactobacillus delbrueckii subsp. bulgaricus
physiological function
cobalamin-specific ECF-type ABC transporter from Lactobacillus delbrueckii, ECF-CbrT, mediates the specific, ATP-dependent uptake of cobalamin. Cobalamin (vitamin B12) is the most complex B-type vitamin and is synthetized exclusively in a limited number of prokaryotes. Its biologically active variants contain rare organometallic bonds, which are used by enzymes in a variety of central metabolic pathways such as L-methionine synthesis and ribonucleotide reduction. Enzyme ECF-CbrT catalyzes ATP-dependent transport of cobalamin and cobinamide
Lactobacillus delbrueckii subsp. bulgaricus
Other publictions for EC 7.6.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749978
Agarwal
Mechanistic basis of vitamin ...
Vibrio cholerae serotype O1, Vibrio cholerae serotype O1 ATCC 39541, Vibrio cholerae serotype O1 Classical Ogawa 395, Vibrio cholerae serotype O1 O395
Biochim. Biophys. Acta
1867
140-151
2019
-
-
1
1
-
-
-
-
1
1
-
16
-
8
-
-
-
-
-
-
-
-
20
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
1
1
-
16
-
-
-
-
-
-
-
-
20
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
749821
Okamoto
Characterization of human ATP ...
Homo sapiens
Biochem. Biophys. Res. Commun.
496
1122-1127
2018
-
-
1
-
-
-
1
-
2
1
-
1
-
3
-
-
1
-
1
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
2
1
-
1
-
-
-
1
1
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
750412
Santos
Functional and structural cha ...
Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842, Lactobacillus delbrueckii subsp. bulgaricus DSM 20081, Lactobacillus delbrueckii subsp. bulgaricus JCM 1002, Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953, Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
eLife
7
e35828
2018
-
-
1
1
-
-
6
1
1
1
-
12
-
8
-
-
-
-
-
-
-
-
18
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
6
-
1
1
1
-
12
-
-
-
-
-
-
-
-
18
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
751644
Schmitt
Vitamin B12 import is all abo ...
Escherichia coli
Nat. Chem. Biol.
14
640-641
2018
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
751670
Rempel
Cysteine-mediated decyanation ...
Thiobacillus denitrificans
Nat. Commun.
9
3038
2018
-
-
1
1
-
-
-
-
1
1
-
2
-
1
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
751661
Goudsmits
Single-molecule visualization ...
Escherichia coli
Nat. Commun.
8
1652
2017
-
-
1
-
5
1
-
-
2
1
-
1
-
1
-
-
-
1
1
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
5
1
-
-
-
-
2
1
-
1
-
-
-
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
752215
Mireku
Structural basis of nanobody- ...
Escherichia coli
Sci. Rep.
7
14296
2017
-
-
1
1
-
-
1
-
1
1
-
1
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
1
-
-
-
-
1
1
1
-
-
-
1
1
-
1
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
752221
Mireku
Conformational change of a tr ...
Escherichia coli
Sci. Rep.
7
41575
2017
-
-
1
1
8
-
-
-
-
1
-
2
-
2
-
-
1
-
1
-
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
8
-
-
-
-
-
-
1
-
2
-
-
-
1
1
-
-
-
3
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
750063
Priess
Release of entropic spring re ...
Escherichia coli
Biophys. J.
110
2407-2418
2016
-
-
-
1
1
-
-
-
1
1
-
1
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
751956
Pan
ATP hydrolysis induced confor ...
Escherichia coli
PLoS ONE
11
e0166980
2016
-
-
-
-
-
-
-
-
2
1
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
752200
Kawaguchi
Translocation of the ABC tran ...
Homo sapiens
Sci. Rep.
6
30183
2016
-
-
1
-
1
-
-
-
3
-
-
1
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
734003
Su
Conformational motions and fun ...
Escherichia coli
Int. J. Mol. Sci.
16
17933-17951
2015
-
-
-
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734281
Joseph
Conformational cycle of the vi ...
Escherichia coli
J. Biol. Chem.
289
3176-3185
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734760
Korkhov
Structure of AMP-PNP-bound Btu ...
Escherichia coli
Nat. Struct. Mol. Biol.
21
1097-1099
2014
-
-
1
1
1
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734820
Gopinath
A vitamin B12 transporter in M ...
Mycobacterium tuberculosis
Open Biology
3
120175
2013
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
719509
Korkhov
Asymmetric states of vitamin B ...
Escherichia coli
FEBS Lett.
586
972-976
2012
-
-
1
1
1
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720849
Weng
-
The conformational transition ...
Escherichia coli
PLoS ONE
7
e305465
2012
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734771
Korkhov
Structure of AMP-PNP-bound vit ...
Escherichia coli
Nature
490
367-372
2012
-
-
-
1
2
-
-
-
1
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719930
Di Bartolo
In vitro folding and assembly ...
Escherichia coli
J. Biol. Chem.
286
18807-18815
2011
-
-
1
-
-
-
2
2
-
-
1
-
-
2
-
-
-
-
1
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
1
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
719987
Joseph
Transmembrane gate movements i ...
Escherichia coli
J. Biol. Chem.
286
41008-41017
2011
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713095
Lewinson
A distinct mechanism for the A ...
Escherichia coli
Nat. Struct. Mol. Biol.
17
332-338
2010
-
-
1
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713485
Kandt
Holo-BtuF stabilizes the open ...
Escherichia coli
Proteins
78
738-753
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713505
Sun
Molecular dynamics simulation ...
Escherichia coli
Sci. China C Life Sci.
53
620-630
2010
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696776
Weng
Asymmetric conformational flex ...
Haemophilus influenzae
Biophys. J.
96
1918-1930
2009
-
-
-
1
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697950
Goetz
Distinct gate conformations of ...
Escherichia coli
FEBS Lett.
583
266-270
2009
-
-
1
1
3
-
-
-
2
1
-
1
-
4
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
-
2
1
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685614
Liu
Study on the mechanism of the ...
Escherichia coli
Biophys. Chem.
135
19-24
2008
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685626
Weng
The conformational coupling an ...
Escherichia coli
Biophys. J.
94
612-621
2008
-
-
-
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685135
Ivetac
Dynamics and function in a bac ...
Escherichia coli
Biochemistry
46
2767-2778
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685616
Sonne
Simulation of the coupling bet ...
Escherichia coli
Biophys. J.
92
2727-2734
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690056
Hvorup
Asymmetry in the structure of ...
Escherichia coli
Science
317
1387-1390
2007
-
-
-
1
-
-
-
-
2
-
-
1
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685066
Kandt
Opening and closing motions in ...
Escherichia coli
Biochemistry
45
13284-13292
2006
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667642
Borths
In vitro functional characteri ...
Escherichia coli
Biochemistry
44
16301-16309
2005
1
-
1
-
-
1
2
-
3
-
-
-
-
2
-
-
1
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
2
-
-
3
-
-
-
-
-
-
1
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210327
Kadner
Vitamin B12 transport in Esche ...
Escherichia coli
Mol. Microbiol.
12
2027-2033
1990
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210328
Rioux
Vitamin B12 transport in Esche ...
Escherichia coli
Mol. Gen. Genet.
217
301-308
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210331
Rioux
Two outer membrane transport s ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
171
2986-2993
1989
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210326
Friedrich
Nucleotide sequence of the btu ...
Escherichia coli
J. Bacteriol.
167
928-934
1986
-
-
1
-
-
-
-
-
2
-
3
1
-
3
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
3
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210329
De Vaux
Identification of the btuCED p ...
Escherichia coli
J. Bacteriol.
167
920-927
1986
-
-
-
-
-
-
-
-
2
-
4
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
4
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210330
De Vaux
Transport of vitamin B12 in Es ...
Escherichia coli
J. Bacteriol.
162
888-896
1985
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-