EC Number |
Cofactor |
Reference |
---|
5.3.3.1 | NADH |
isomerase activity of enzyme requires NADH, Km- and kcat-value of isoform 1 wild-type are 0.0046 mM and 45 per min, resp. Km- and kcat-value of isoform 2 wild-type are 0.0126 mM and 99.1 per min, resp |
662371 |
5.3.3.1 | NADH |
Km value 0.0126 mM, pH 7.4, 27°C |
693887 |
5.3.3.1 | NADH |
Km value of wild-type 0.0046 mM, pH 7.4, 27°C |
693887 |
5.3.3.1 | NADH |
the cofactor acts as an allosteric activator |
670028 |
5.3.3.1 | NADH |
the isomerase activity of the wild-type enzyme and the deletion mutant enzyme DELTA283-310 requires allosteric activation by NADH, Km for wild-type enzyme and deletion mutant enzyme is 0.0046 mM |
389386 |
5.3.3.1 | NADH |
wild-type 1 isomerase is activated only by NADH with NADPH producing no isomerase activity. D36A/K37R mutation shifts the cofactor preference of both 3-beta-hydroxysteroid dehydrogenase and isomerase from NAD(H) to NADP(H) |
652452 |
5.3.3.1 | NADPH |
D36A/K37R mutation shifts the cofactor preference of both 3-beta-hydroxysteroid dehydrogenase and isomerase from NAD(H) to NADP(H) |
652452 |