EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference  |
|---|
    5.4.99.1 | 5.8 |
- |
(2S,3S)-3-methylaspartate |
genetically engineered enzyme with S subunit fused to the C-terminus of the E subunit through an 11 amino acid (Gly-Gln)5-Gly linker segment |
3455 |
| 5.4.99.1 | 5.8 |
- |
L-Glu |
genetically engineered enzyme with S subunit fused to the C-terminus of the E subunit through an 11 amino acid (Gly-Gln)5-Gly linker segment |
3455 |
| 5.4.99.1 | 0.65 |
- |
L-Glu |
reaction of L-Glu with benzimidazolribofuranosyl-adenosylcobinamide, fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit |
3458 |
| 5.4.99.1 | 20 |
- |
L-Glu |
reaction of L-Glu with benzimidazolribofuranosyl-adenosylcobinamide, wild-type enzyme |
3458 |
| 5.4.99.1 | 0.05 |
- |
L-2-hydroxyglutarate |
pH 7.0 |
649913 |
| 5.4.99.1 | 0.0181 |
- |
L-glutamate |
25°C, mutant enzyme R100Y |
650247 |
| 5.4.99.1 | 0.0211 |
- |
L-glutamate |
25°C, mutant enzyme R100M |
650247 |
| 5.4.99.1 | 0.048 |
- |
L-glutamate |
25°C, mutant enzyme R100K |
650247 |
| 5.4.99.1 | 5.8 |
- |
L-glutamate |
25°C, wild-type enzyme |
650247 |
| 5.4.99.1 | 0.025 |
- |
L-glutamate |
23°C, mutant enzyme E171N |
650824 |
