EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    4.1.3.1 | 0.56 |
- |
isocitrate |
recombinant His-tagged mutant N214D, pH 7.8, 30°C |
728845 |
| 4.1.3.1 | 1.25 |
- |
(2R,3S:2S,3R)-2-methyl-isocitrate |
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 |
681995 |
| 4.1.3.1 | 1.38 |
- |
isocitrate |
ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2 |
681995 |
| 4.1.3.1 | 3.25 |
- |
isocitrate |
recombinant His-tagged mutant Q221K, pH 7.8, 30°C |
728845 |
| 4.1.3.1 | 3.46 |
- |
isocitrate |
recombinant His-tagged mutant Q211H, pH 7.8, 30°C |
728845 |
| 4.1.3.1 | 3.6 |
- |
glyoxylate |
pH 7.5, 25°C, recombinant enzyme |
727039 |
| 4.1.3.1 | 3.6 |
- |
succinate |
pH 7.5, 25°C, recombinant enzyme |
727039 |
| 4.1.3.1 | 4.4 |
- |
isocitrate |
mutant enzyme T534A, at pH 7.0 and 37°C |
748505 |
| 4.1.3.1 | 4.63 |
- |
isocitrate |
recombinant His-tagged mutant E219A, pH 7.8, 30°C |
728845 |
| 4.1.3.1 | 5.2 |
- |
isocitrate |
pH 7.5, 25°C, recombinant enzyme |
727039 |
