EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   4.1.2.48 | 0.000107 |
- |
L-phenylserine |
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.000113 |
- |
L-phenylserine |
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.00022 |
- |
L-threo-3,4-dihydroxyphenylserine |
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.00023 |
- |
L-threo-3,4-dihydroxyphenylserine |
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.0073 |
- |
L-threonine |
pH 7.0, 30°C, mutant enzyme H83F/H126F |
747734 |
| 4.1.2.48 | 0.0178 |
- |
L-Thr |
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.0195 |
- |
L-Thr |
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.02 |
- |
L-threonine |
mutant H83F, pH 7.0, 30°C |
727485 |
| 4.1.2.48 | 0.028 |
- |
L-allo-threonine |
pH 7.0, 30°C, mutant enzyme H83F/H126F |
747734 |
| 4.1.2.48 | 0.06 |
- |
L-allo-threonine |
mutant H83F, pH 7.0, 30°C |
727485 |
