Application | Comment | Organism |
---|---|---|
synthesis | the enzyme may be exploited for bioorganic synthesis of L-3-hydroxyamino acids that are biologically active or constitute building blocks for pharmaceutical molecules | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of the low-specificity L-threonine aldolase is determined at 2.2 A resolution, in the unliganded form and co-crystallized with L-serine and L-threonine | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F87A | catalytic efficiency of the mutant enzyme for L-threonine is 1.7fold lower than that of the wild-type enzyme, catalytic efficiency of the mutant enzyme for L-allo-threonine is 1.2fold lower than that of the wild-type enzyme | Escherichia coli |
F87D | catalytic efficiency of the mutant enzyme for L-threonine is 3.11fold lower than that of the wild-type enzyme, catalytic efficiency of the mutant enzyme for L-allo-threonine is 7.5fold lower than that of the wild-type enzyme | Escherichia coli |
H83F/H126F | catalytic efficiency of the mutant enzyme for L-threonine is 3890fold lower than that of the wild-type enzyme, catalytic efficiency of the mutant enzyme for L-allo-threonine is 294fold lower than that of the wild-type enzyme | Escherichia coli |
K222A | catalytic efficiency of the mutant enzyme for L-threonine is 13.4fold lower than that of the wild-type enzyme, catalytic efficiency of the mutant enzyme for L-allo-threonine is 9.7fold lower than that of the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-allo-threonine | pH 7.0, 30°C, wild-type enzyme | Escherichia coli | |
0.31 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme F87A | Escherichia coli | |
0.41 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme F87D | Escherichia coli | |
1.2 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme K222A | Escherichia coli | |
7.4 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme H83F/H126F | Escherichia coli | |
8.7 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme F87A | Escherichia coli | |
19.4 | - |
L-threonine | pH 7.0, 30°C, wild-type enzyme | Escherichia coli | |
21 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme H83F/H126F | Escherichia coli | |
24.9 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme F87D | Escherichia coli | |
72 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme K222A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P75823 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-allo-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
? | |
L-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Low-specificity L-threonine aldolase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0073 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme H83F/H126F | Escherichia coli | |
0.028 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme H83F/H126F | Escherichia coli | |
0.33 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme F87D | Escherichia coli | |
0.72 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme F87A | Escherichia coli | |
0.72 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme K222A | Escherichia coli | |
1.33 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme K222A | Escherichia coli | |
1.87 | - |
L-threonine | pH 7.0, 30°C, wild-type enzyme | Escherichia coli | |
1.95 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme F87D | Escherichia coli | |
2.7 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme F87A | Escherichia coli | |
3.55 | - |
L-allo-threonine | pH 7.0, 30°C, wild-type enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme H83F/H126F | Escherichia coli | |
0.0038 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme H83F/H126F | Escherichia coli | |
0.01 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme K222A | Escherichia coli | |
0.013 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme F87D | Escherichia coli | |
0.082 | - |
L-allo-threonine | pH 7.0, 30°C, mutant enzyme F87A | Escherichia coli | |
0.097 | - |
L-allo-threonine | pH 7.0, 30°C, wild-type enzyme | Escherichia coli | |
1.1 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme K222A | Escherichia coli | |
4.75 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme F87D | Escherichia coli | |
8.6 | - |
L-threonine | pH 7.0, 30°C, mutant enzyme F87A | Escherichia coli | |
14.78 | - |
L-threonine | pH 7.0, 30°C, wild-type enzyme | Escherichia coli |