EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.5.2.6 | -999 |
- |
aztreonam |
unable to determine as Km is too high |
692632 |
| 3.5.2.6 | -999 |
- |
cefepime |
above 8, enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin |
692632 |
| 3.5.2.6 | -999 |
- |
ertapenem |
above 25, enzyme diluted in HEPES buffer containing 50 microM ZnCl2 supplemented with bovine serum albumin |
692632 |
| 3.5.2.6 | -999 |
- |
more |
no hydrolysis detected with enzyme concentrations up to 4 microM for temocillin, cefepime, imipenem, and aztreonam |
695634 |
| 3.5.2.6 | 0.0002 |
- |
cefotaxime |
above, wild-type, 30°C, pH 6.5 |
696094 |
| 3.5.2.6 | 0.0002 |
- |
nitrocefin |
D120T mutant, 30°C, pH 6.5 |
696094 |
| 3.5.2.6 | 0.00024 |
- |
Imipenem |
in 0.01 M phosphate buffer (pH 7.0), at 25°C |
687112 |
| 3.5.2.6 | 0.00033 |
- |
doripenem |
pH 6.5, 22°C, recombinant enzyme |
733349 |
| 3.5.2.6 | 0.00033 |
- |
ertapenem |
pH 6.5, 22°C, recombinant enzyme |
733349 |
| 3.5.2.6 | 0.00043 |
- |
cefoxitin |
in 0.01 M phosphate buffer (pH 7.0), at 25°C |
687112 |
