EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate  |
Reference |
|---|
   3.2.1.18 | 2.9 |
- |
(trifluoromethyl)umbelliferyl alpha-sialoside |
wild-type, hydrolysis, pH 7.6, 25°C |
696254 |
| 3.2.1.18 | 0.045 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
mutant enzyme H275Y/I223V, at 37°C, pH not specified in the publication |
752324 |
| 3.2.1.18 | 0.054 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
mutant enzyme H275Y, at 37°C, pH not specified in the publication |
752324 |
| 3.2.1.18 | 0.0743 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
pH 3.5, 37°C |
717646 |
| 3.2.1.18 | 0.1 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
mutant enzyme H275Y/S247N, at 37°C, pH not specified in the publication |
752324 |
| 3.2.1.18 | 0.17 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
mutant enzyme S247N, at 37°C, pH not specified in the publication |
752324 |
| 3.2.1.18 | 0.6 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
mutant enzyme I223V, at 37°C, pH not specified in the publication |
752324 |
| 3.2.1.18 | 0.9 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
wild type enzyme, at 37°C, pH not specified in the publication |
752324 |
| 3.2.1.18 | 8 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
recombinant mutant M87G, pH 5.0, 37°C |
717641 |
| 3.2.1.18 | 23 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
recombinant wild-type enzyme, pH 5.0, 37°C |
717641 |
