EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference  |
|---|
   3.2.1.18 | 45 |
- |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid |
- |
171170 |
| 3.2.1.18 | -999 |
- |
more |
kcat at pH 9.5, 37°C, relative to the reported kcat at pH 6.0, 37°C, with substrate 4-nitrophenyl alpha-D-N-acetylneurminide |
655779 |
| 3.2.1.18 | 0.82 |
- |
phenyl alpha-sialoside |
pH 5.25, 37°C, mutant enzyme Y370G |
678161 |
| 3.2.1.18 | 13.3 |
- |
phenyl beta-sialoside |
pH 5.25, 37°C, mutant enzyme Y370G |
678161 |
| 3.2.1.18 | 0.031 |
- |
4-nitrophenyl alpha-sialoside |
mutant D59A, hydrolysis, pH 7.6, 25°C |
696254 |
| 3.2.1.18 | 1.57 |
- |
4-nitrophenyl alpha-sialoside |
wild-type, hydrolysis, pH 7.6, 25°C |
696254 |
| 3.2.1.18 | 2.9 |
- |
(trifluoromethyl)umbelliferyl alpha-sialoside |
wild-type, hydrolysis, pH 7.6, 25°C |
696254 |
| 3.2.1.18 | 0.88 |
- |
4-nitrophenyl alpha-D-N-acetylneuraminic acid |
- |
703911 |
| 3.2.1.18 | 8 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
recombinant mutant M87G, pH 5.0, 37°C |
717641 |
| 3.2.1.18 | 23 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
recombinant wild-type enzyme, pH 5.0, 37°C |
717641 |
