EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.1.3.75 | 0.2 |
- |
p-nitrophenyl phosphate |
recombinant mutant enzyme D267E, at pH 5.0 and 37°C, in the presence of 2 mM Mg2+ |
696420 |
3.1.3.75 | 0.7 |
- |
p-nitrophenyl phosphate |
recombinant mutant enzyme D267E, at pH 5.0 and 37°C, in the presence of 0.5 mM Zn2+ |
696420 |
3.1.3.75 | 1.04 |
- |
phosphocholine |
50 mM Hepes/NaOH buffer, pH 7.0, 37°C |
714360 |
3.1.3.75 | 1.98 |
- |
phosphocholine |
pH 6.7, 37°C, 2 mM Mg2+ |
654549 |
3.1.3.75 | 2.27 |
- |
O-Phosphoethanolamine |
pH 6.7, 37°C, 2 mM Mg2+ |
654549 |
3.1.3.75 | 6.7 |
- |
4-nitrophenyl phosphate |
pH 5.0, 37°C, recombinant mutant Y82A/Y83A/Y84A |
714357 |
3.1.3.75 | 13 |
- |
Phosphoethanolamine |
50 mM Hepes/NaOH buffer, pH 7.0, 37°C |
714360 |
3.1.3.75 | 38.8 |
- |
4-nitrophenyl phosphate |
pH 5.0, 37°C, recombinant mutant E42A |
714357 |
3.1.3.75 | 79.6 |
- |
4-nitrophenyl phosphate |
pH 5.0, 37°C, recombinant mutant E43A |
714357 |
3.1.3.75 | 83 |
- |
p-nitrophenyl phosphate |
- |
706604 |