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Literature summary for 3.1.3.75 extracted from

  • Beassoni, P.R.; Berti, F.P.; Otero, L.H.; Risso, V.A.; Ferreyra, R.G.; Lisa, A.T.; Domenech, C.E.; Ermacora, M.R.
    Preparation and biophysical characterization of recombinant Pseudomonas aeruginosa phosphorylcholine phosphatase (2010), Protein Expr. Purif., 71, 153-159.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C87A involved in a non-essential intramolecular disulfide bond Pseudomonas aeruginosa
C94A involved in a non-essential intramolecular disulfide bond Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
p-nitrophenyl phosphate
-
Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37089
-
2 * 37089, MS, multiangle light scattering Pseudomonas aeruginosa
74600
-
multiangle light scattering Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HTR2
-
-

Purification (Commentary)

Purification (Comment) Organism
from solubilized inclusion bodies, ion-exchange chromatography (Q-Sepharose), dialysis, gel filtration Pseudomonas aeruginosa

Renatured (Commentary)

Renatured (Comment) Organism
dialysis, buffer with 5 mM Mg2+ Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
Pseudomonas aeruginosa p-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 37089, MS, multiangle light scattering Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
phosphorylcholine phosphatase
-
Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
apparent Tm value: 65.8°C (5 mM Mg2+) Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83
-
p-nitrophenyl phosphate
-
Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
maximum of kcat-value and minimum for KM-value Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
24
-
p-nitrophenyl phosphate C94A mutant protein Pseudomonas aeruginosa
29
-
p-nitrophenyl phosphate wild-type protein Pseudomonas aeruginosa
44
-
p-nitrophenyl phosphate C87A mutant protein Pseudomonas aeruginosa