Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging drop method | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C87A | involved in a non-essential intramolecular disulfide bond | Pseudomonas aeruginosa |
C94A | involved in a non-essential intramolecular disulfide bond | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
p-nitrophenyl phosphate | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37089 | - |
2 * 37089, MS, multiangle light scattering | Pseudomonas aeruginosa |
74600 | - |
multiangle light scattering | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HTR2 | - |
- |
Purification (Comment) | Organism |
---|---|
from solubilized inclusion bodies, ion-exchange chromatography (Q-Sepharose), dialysis, gel filtration | Pseudomonas aeruginosa |
Renatured (Comment) | Organism |
---|---|
dialysis, buffer with 5 mM Mg2+ | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Pseudomonas aeruginosa | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37089, MS, multiangle light scattering | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
phosphorylcholine phosphatase | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
apparent Tm value: 65.8°C (5 mM Mg2+) | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83 | - |
p-nitrophenyl phosphate | - |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
maximum of kcat-value and minimum for KM-value | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
p-nitrophenyl phosphate | C94A mutant protein | Pseudomonas aeruginosa | |
29 | - |
p-nitrophenyl phosphate | wild-type protein | Pseudomonas aeruginosa | |
44 | - |
p-nitrophenyl phosphate | C87A mutant protein | Pseudomonas aeruginosa |