EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.3.2.6 | 0.6 |
- |
alanine-4-nitroanilide |
pH 7.6, 22°C |
646992 |
3.3.2.6 | 0.56 |
- |
Arg-Ser-Arg |
wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) |
697288 |
3.3.2.6 | 0.001 |
- |
L-Ala-p-nitroanilide |
mutant enzyme E296Q, 500 mM KCl in 250 mM Tris buffer (pH 7.5) |
697288 |
3.3.2.6 | 0.5 |
- |
L-Ala-p-nitroanilide |
mutant enzyme D375A, 500 mM KCl in 250 mM Tris buffer (pH 7.5) |
697288 |
3.3.2.6 | 1.7 |
- |
L-Ala-p-nitroanilide |
wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) |
697288 |
3.3.2.6 | 0.0005 |
- |
L-alanine 2-naphthylamide |
mutant N362L, 25°C, pH not specified in the publication |
714325 |
3.3.2.6 | 0.003 |
- |
L-alanine 2-naphthylamide |
mutant N362E, 25°C, pH not specified in the publication |
714325 |
3.3.2.6 | 0.013 |
- |
L-alanine 2-naphthylamide |
mutant N362Q, 25°C, pH not specified in the publication |
714325 |
3.3.2.6 | 0.03 |
- |
L-alanine 2-naphthylamide |
wild-type, 25°C, pH not specified in the publication |
714325 |
3.3.2.6 | 0.065 |
- |
L-alanine 2-naphthylamide |
mutant D399A, 25°C, pH not specified in the publication |
714325 |