KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | influence of NaCl and NaSCN on KM | Homo sapiens | |
0.1 | - |
proline-4-nitroanilide | pH 7.6, 22°C | Homo sapiens | |
0.1 | - |
lysine-4-nitroanilide | pH 7.6, 22°C | Homo sapiens | |
0.2 | - |
L-arginine-4-nitroanilide | pH 7.6, 22°C | Homo sapiens | |
0.3 | - |
L-leucine-4-nitroanilide | pH 7.6, 22°C | Homo sapiens | |
0.5 | - |
alanine-4-nitroanilide | pH 7.6, 22°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Br- | stimulates peptidase and epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- | Homo sapiens | |
Cl- | stimulates peptidase activity, not epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- | Homo sapiens | |
thiocyanate | stimulates peptidase and epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | possible proteolytic function of the enzyme may be limited to the extracellular space | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine-4-nitroanilide + H2O | - |
Homo sapiens | L-arginine + 4-nitroaniline | - |
? | |
L-leucine-4-nitroanilide + H2O | - |
Homo sapiens | L-leucine + 4-nitroaniline | - |
? | |
leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? | |
lysine-4-nitroanilide + H2O | - |
Homo sapiens | lysine + 4-nitroaniline | - |
? | |
additional information | not: glycine-4-nitroanilide, glutamyl-4-nitroanilide, valine-4-nitroanilide | Homo sapiens | ? | - |
? | |
additional information | possible proteolytic function of the enzyme may be limited to the extracellular space | Homo sapiens | ? | - |
? | |
proline-4-nitroanilide + H2O | - |
Homo sapiens | proline + 4-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
22°C: 53% of activity maximum, 50°C: 33% of activity maximum | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
lysine-4-nitroanilide | pH 7.6, 22°C | Homo sapiens | |
0.15 | - |
L-leucine-4-nitroanilide | pH 7.6, 22°C | Homo sapiens | |
0.15 | - |
proline-4-nitroanilide | pH 7.6, 22°C | Homo sapiens | |
0.15 | - |
L-arginine-4-nitroanilide | pH 7.6, 22°C | Homo sapiens | |
0.15 | 2.4 | leukotriene A4 | value depending on NaSCN concentration | Homo sapiens | |
0.6 | - |
alanine-4-nitroanilide | pH 7.6, 22°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | about 25% of activity maximum at pH 6.5 and 8.5 | Homo sapiens |