Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.3.2.6 extracted from

  • Wetterholm, A.; Haeggström, J.Z.
    Leukotriene A4 hydrolase: an anion activated peptidase (1992), Biochim. Biophys. Acta, 1123, 275-281.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information influence of NaCl and NaSCN on KM Homo sapiens
0.1
-
proline-4-nitroanilide pH 7.6, 22°C Homo sapiens
0.1
-
lysine-4-nitroanilide pH 7.6, 22°C Homo sapiens
0.2
-
L-arginine-4-nitroanilide pH 7.6, 22°C Homo sapiens
0.3
-
L-leucine-4-nitroanilide pH 7.6, 22°C Homo sapiens
0.5
-
alanine-4-nitroanilide pH 7.6, 22°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Br- stimulates peptidase and epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- Homo sapiens
Cl- stimulates peptidase activity, not epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- Homo sapiens
thiocyanate stimulates peptidase and epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br- Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens possible proteolytic function of the enzyme may be limited to the extracellular space ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine-4-nitroanilide + H2O
-
Homo sapiens L-arginine + 4-nitroaniline
-
?
L-leucine-4-nitroanilide + H2O
-
Homo sapiens L-leucine + 4-nitroaniline
-
?
leukotriene A4 + H2O
-
Homo sapiens leukotriene B4
-
?
lysine-4-nitroanilide + H2O
-
Homo sapiens lysine + 4-nitroaniline
-
?
additional information not: glycine-4-nitroanilide, glutamyl-4-nitroanilide, valine-4-nitroanilide Homo sapiens ?
-
?
additional information possible proteolytic function of the enzyme may be limited to the extracellular space Homo sapiens ?
-
?
proline-4-nitroanilide + H2O
-
Homo sapiens proline + 4-nitroaniline
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22
-
22°C: 53% of activity maximum, 50°C: 33% of activity maximum Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
lysine-4-nitroanilide pH 7.6, 22°C Homo sapiens
0.15
-
L-leucine-4-nitroanilide pH 7.6, 22°C Homo sapiens
0.15
-
proline-4-nitroanilide pH 7.6, 22°C Homo sapiens
0.15
-
L-arginine-4-nitroanilide pH 7.6, 22°C Homo sapiens
0.15 2.4 leukotriene A4 value depending on NaSCN concentration Homo sapiens
0.6
-
alanine-4-nitroanilide pH 7.6, 22°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 about 25% of activity maximum at pH 6.5 and 8.5 Homo sapiens