EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.1.1.14 | 0.283 |
- |
5-methyltetrahydropteroyl-gamma-glutamylglutamate |
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C |
671587 |
2.1.1.14 | 0.33 |
- |
L-homocysteine |
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C |
671587 |
2.1.1.14 | 0.35 |
- |
5-methyltetrahydropteroyl-gamma-glutamylglutamate |
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C |
671587 |
2.1.1.14 | 0.4 |
- |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate |
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C |
671587 |
2.1.1.14 | 0.417 |
- |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate |
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C |
671587 |
2.1.1.14 | 0.417 |
- |
L-homocysteine |
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C |
671587 |
2.1.1.14 | 0.533 |
- |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate |
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C |
671587 |
2.1.1.14 | 0.6 |
- |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate |
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C |
671587 |
2.1.1.14 | 0.12 |
- |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate |
at 25°C |
672156 |
2.1.1.14 | 0.45 |
- |
L-homocysteine |
37°C, pH not specified in the publication |
734490 |