EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.1.31.1 | -999 |
- |
- |
135023 |
| 3.1.31.1 | -999 |
- |
2-O-alpha-mannosylglycerate protects against thermal denaturation. 0.5 M 2-O-alpha-mannosylglycerate increases Tm-value by 7.1°C. 0.5 M glycerol or trehalose increase the Tm-value by 0.8°C and 4.2°C, respectively |
665585 |
| 3.1.31.1 | -999 |
- |
different concentrations of mannosylglycerate or presence of urea at 0.25M show no correlation with changes in the thermodynamic stability of the P117G/H124L/S128A mutant enzyme |
730894 |
| 3.1.31.1 | -999 |
- |
examination of denaturation (monitored by intrinsic fluorescence of Trp140) due to heat (pH 7): D21N/T33V/T41I/S59A/P117G/A128A mutant(Tm = 72°C), K9A mutant (Tm = 71°C), E73A mutant (Tm = 67°C), E75A mutant (Tm = 70°C), E75Q mutant (Tm = 70°C), D77A mutant (Tm = 67°C), Y91A mutant (Tm = 58°C), Y93A mutant (Tm = 52°C), E101A mutant (Tm = 71°C), K127A mutant (Tm = 75°C), A128S mutant (Tm = 71°C) |
699534 |
| 3.1.31.1 | -999 |
- |
heat-stable extracellular enzyme related structurally to a heat-labile cellular nuclease |
135033 |
| 3.1.31.1 | -999 |
- |
temperature- and pressure-dependent stability diagram, thermodynamic properties, and folding reaction kinetics of the small, single-domain monomeric staphylococcal nuclease in presence or absence of macromolecular crowder agent Ficoll PM 70, which behaves as a semi-rigid spheroid with a hydrodynamic radius of about 5 nm, detailed overview. Increasing the temperature from 25°C to 57°C exhibits no change of the size of the crowder particles within the experimentalerror. No significant changes in particle size are detected by raising the pressure up to 2.5 kbar. The measured translational diffusion times indicate that, depending on the size of the protein, themicroviscosity of Ficoll PM70 is only about 4-6 times larger than the microviscosity of pure water |
730556 |
| 3.1.31.1 | -999 |
- |
thermostable enzyme |
730338 |
| 3.1.31.1 | 15 |
80 |
temperature range for studying the thermal unfolding transitions |
681430 |
| 3.1.31.1 | 39 |
- |
Tm-value of mutant enzyme W140F is 39.1°C, Tm-value of mutant enzyme W140Y is 38.6°C |
666872 |
| 3.1.31.1 | 42 |
- |
Tm-value of mutant enzyme is 41.8°C |
666872 |
