Protein Variants | Comment | Organism |
---|---|---|
W140A | DNA hydrolysis activity is 70% of wild-type activity | Staphylococcus sp. |
W140C | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140D | DNA hydrolysis activity is 65% of wild-type activity | Staphylococcus sp. |
W140E | DNA hydrolysis activity is 65% of wild-type activity | Staphylococcus sp. |
W140F | native-like structure and native-like activity under physiological conditions | Staphylococcus sp. |
W140G | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140H | native-like structure and native-like activity under physiological conditions | Staphylococcus sp. |
W140I | DNA hydrolysis activity is 70% of wild-type activity | Staphylococcus sp. |
W140K | DNA hydrolysis activity is 70% of wild-type activity | Staphylococcus sp. |
W140L | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140M | DNA hydrolysis activity is 70% of wild-type activity | Staphylococcus sp. |
W140N | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140P | DNA hydrolysis activity is 55% of wild-type activity | Staphylococcus sp. |
W140Q | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140R | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140S | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140T | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140V | DNA hydrolysis activity is 75% of wild-type activity | Staphylococcus sp. |
W140Y | native-like structure and native-like activity under physiological conditions | Staphylococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Staphylococcus sp. | 3'-deoxymononucleotides + dinucleotides | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
Tm-value of mutant enzyme W140F is 39.1°C, Tm-value of mutant enzyme W140Y is 38.6°C | Staphylococcus sp. |
42 | - |
Tm-value of mutant enzyme is 41.8°C | Staphylococcus sp. |
51 | - |
Tm-value of wild-type enzyme is 50.7°C | Staphylococcus sp. |