EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.1.30.1 | 30 |
70 |
50% of activity with ssDNA at 47°C, with dsDNA at 49°C |
134988 |
| 3.1.30.1 | 30 |
70 |
at 70°C almost 75% of initial activity with immobilized, 20% with soluble enzyme |
134981 |
| 3.1.30.1 | 50 |
- |
40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min |
134996 |
| 3.1.30.1 | 55 |
65 |
no inactivation by heating at 55°C, partial inactivation at 65°C, importance of high content of hydrophobic amino acids and zinc atoms for thermostability postulated |
134972 |
| 3.1.30.1 | 60 |
- |
after 90 min incubation time 90% of activity towards 3'-AMP and 60% of activity towards DNA lost |
95076 |
| 3.1.30.1 | 60 |
67 |
stable below 60°C, 50% inactivation after heating at 67°C for 15 min, pH 6.0 |
134971 |
| 3.1.30.1 | 60 |
70 |
stable to heating at pH 5.0 in the presence of both Zn2+ and sulfhydryl compounds |
134971 |
| 3.1.30.1 | 60 |
80 |
at 70°C complete loss of activity of the soluble enzyme, 40% of maximal activity of the immobilized enzyme at this temperature, 20% at 80°C |
134974 |
| 3.1.30.1 | 65 |
- |
no inactivation of ribonuclease and deoxyribonuclease activity by heating at this temperature in the presence of substrate |
134971 |
| 3.1.30.1 | 74 |
- |
in 0.02 M potassium phosphate buffer, pH 6.5, 37% of activity towards dsDNA after heating of 2 min, no activity after heating of 10 min |
134986 |
