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Literature summary for 3.1.30.1 extracted from

  • Gite, S.; Shankar, V.
    Preparation, properties and application of Aspergillus oryzae S1 nuclease covalently bound to aminobutyl-Bio-Gel P-2 through its carbohydrate moiety (1993), Biotechnol. Appl. Biochem., 17, 373-382.
    View publication on PubMed
Search for more literature for 3.1.30.1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus oryzae

Storage Stability

Storage Stability Organism
4°C, 30 mM sodium acetate buffer pH 4.6, containing 1 mM ZnSO4, 50 mM NaCl and 5% glycerol, more than 45 days, no apparent loss of activity Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
ssDNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

Synonyms Comment Organism
S1 nuclease
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Aspergillus oryzae
55
-
 soluble and immobilized enzyme Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 at 70°C almost 75% of initial activity with immobilized, 20% with soluble enzyme Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
-
 Aspergillus oryzae