EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.6.1.2 | -999 |
- |
NADPH protects native enzyme from thermal inactivation, NADP+ accelerates thermal inactivation, both native and S-thionitrobenzoate derivatized enzyme are protected by Mg2+ against thermoinactivation |
392644 |
1.6.1.2 | -999 |
- |
protection against thermal inactivation by cations, half-maximal protection is afforded at: 1-2 mM Mg2+, 2 mM Ca2+, 0.5 mM Mn2+ and 0.025 mM La3+ |
392634 |
1.6.1.2 | -999 |
- |
proteolytic inactivation as a function of temperature |
392633 |
1.6.1.2 | 40 |
- |
5 min, soluble component, complete inactivation |
392649 |
1.6.1.2 | 44 |
- |
2 min, solubilized membrane component, 50% inactivation |
392641 |
1.6.1.2 | 45 |
- |
incubation of depleted membranes, inactivation of membrane transhydrogenase component |
392647 |
1.6.1.2 | 48 |
- |
2 min, membrane particles, 50% inactivation |
392641 |
1.6.1.2 | 50 |
- |
complete protection in the presence of NADPH, half-maximal protection with 0.01 mM NADPH |
392647 |
1.6.1.2 | 53 |
- |
incubation for 3 min, 10 mM Mg2+ almost completely protect from inactivation |
392647 |