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Literature summary for 1.6.1.2 extracted from
- Resolution and reconstitution of Rhodospirillum rubrum pyridine dinucleotide transhydrogenase. Proteolytic and thermal inactivation of the membrane component (1975), J. Biol. Chem., 250, 819-825.
General Stability
| General Stability | Organism |
|---|---|
| trypsinolysis is stimulated several fold by NADPH and NADP+, half-maximal stimulation with 0.001-0.002 mM NADPH and 0.002-0.003 mM NADP+, Mg2+ protects from NADP+ stimulated inactivation | Rhodospirillum rubrum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1 mM, half-maximal inhibition, 10 mM, 80% inhibition | Rhodospirillum rubrum |  |
| palmitoyl-CoA | - |
Rhodospirillum rubrum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
- |
- |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
incubation of depleted membranes, inactivation of membrane transhydrogenase component | Rhodospirillum rubrum |
| 50 | - |
complete protection in the presence of NADPH, half-maximal protection with 0.01 mM NADPH | Rhodospirillum rubrum |
| 53 | - |
incubation for 3 min, 10 mM Mg2+ almost completely protect from inactivation | Rhodospirillum rubrum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rhodospirillum rubrum | |
| NADH | - |
Rhodospirillum rubrum | |
| NADP+ | - |
Rhodospirillum rubrum | |
| NADPH | - |
Rhodospirillum rubrum | |
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