EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.6.1.1 | 4 |
50 |
the enzyme retains 50% activity after 5 h at 50°C. The enzyme is stable at 4°C for 25 days and retains 65% activity at 25°C. The enzyme is rapidly inactivated at high temperatures, retaining 80%, 50% and 10% activity after incubation for 30 min at 50, 57 and 62°C, respectively |
725040 |
| 1.6.1.1 | 50 |
- |
1 h stable, inactivation is dramatically accelerated by NADH and NADPH, partial protection by NADP+ and FMN, almost full protection by FAD |
392586 |
| 1.6.1.1 | 50 |
- |
purified enzyme, pH 7.5, 5h, 50% activity remaining, 80% activity remaining after 30 min |
725040 |
| 1.6.1.1 | 51 |
- |
25 min, 50% inactivation, accelerated by addition of NADPH, reactivation by FAD |
392590 |
| 1.6.1.1 | 55 |
- |
approx. 50% activity lost after about 2 min, almost complete loss of activity after 20 min, biphasic inactivation: 70% activity lost with a first-order inactivation constant, 30% is lost much more rapidly, rate of thermal inactivation depends on concentration of NAD+, NADP+, NADH, NADPH, free FAD, Mg2+ and phosphate, independent of pH between pH 5 and pH 9, significant acceleration outside this range, addition of 1 mM FAD lowers inactivation rate about 20fold |
392575 |
| 1.6.1.1 | 57 |
- |
purified enzyme, pH 7.5, 30 min, 50% activity remaining |
725040 |
| 1.6.1.1 | 62 |
- |
purified enzyme, pH 7.5, 30 min, 10% activity remaining |
725040 |
| 1.6.1.1 | 65 |
- |
15 min, complete inactivation, protection by FAD |
392586 |
