BRENDA - Enzyme Database
show all sequences of 1.6.1.1

Purification and properties of the pyridine nucleotide transhydrogenase from Pseudomonas aeruginosa

Cohen, P.T.; Kaplan, N.O.; J. Biol. Chem. 245, 2825-2836 (1970) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
p-hydroxymercuribenzoate
-
Pseudomonas aeruginosa
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
NADP+ + NADH
diaphorase-type reactions with NAD(P)H, K3Fe(CN)6 and 2,6-dichlorophenol indophenol
392590
Pseudomonas aeruginosa
NADPH + NAD+
-
392590
Pseudomonas aeruginosa
?
NADPH + 3-acetylpyridine-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + 3-acetylpyridine-NADH
-
392590
Pseudomonas aeruginosa
?
NADPH + deamino-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + deamino-NADH
-
392590
Pseudomonas aeruginosa
?
NADPH + pyridine aldehyde-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + pyridine aldehyde-NADH
-
392590
Pseudomonas aeruginosa
?
NADPH + thio-NADP+ + H+[side 1]
-
392590
Pseudomonas aeruginosa
NADP+ + thio-NADPH + H+[side 2]
-
392590
Pseudomonas aeruginosa
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
51
-
25 min, 50% inactivation, accelerated by addition of NADPH, reactivation by FAD
Pseudomonas aeruginosa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
233
333
NADPH
-
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-hydroxymercuribenzoate
-
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
NADP+ + NADH
diaphorase-type reactions with NAD(P)H, K3Fe(CN)6 and 2,6-dichlorophenol indophenol
392590
Pseudomonas aeruginosa
NADPH + NAD+
-
392590
Pseudomonas aeruginosa
?
NADPH + 3-acetylpyridine-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + 3-acetylpyridine-NADH
-
392590
Pseudomonas aeruginosa
?
NADPH + deamino-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + deamino-NADH
-
392590
Pseudomonas aeruginosa
?
NADPH + pyridine aldehyde-NAD+
-
392590
Pseudomonas aeruginosa
NADP+ + pyridine aldehyde-NADH
-
392590
Pseudomonas aeruginosa
?
NADPH + thio-NADP+ + H+[side 1]
-
392590
Pseudomonas aeruginosa
NADP+ + thio-NADPH + H+[side 2]
-
392590
Pseudomonas aeruginosa
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
51
-
25 min, 50% inactivation, accelerated by addition of NADPH, reactivation by FAD
Pseudomonas aeruginosa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
233
333
NADPH
-
Pseudomonas aeruginosa
Other publictions for EC 1.6.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743251
Haverkorn van Rijsewijk
Distinct transcriptional regu ...
Escherichia coli, Escherichia coli BW25113
Microbiology
162
1672-1679
2016
-
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742182
Jan
Metabolic engineering and tra ...
Escherichia coli
Biotechnol. Prog.
29
1124-1130
2013
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1
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1
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742371
Yamaguchi
A novel homozygous mutation o ...
Homo sapiens
Endocr. J.
60
855-859
2013
-
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1
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1
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-
-
-
-
725040
Cao
Overexpression and biochemical ...
Escherichia coli
FEMS Microbiol. Lett.
320
9-14
2011
6
-
2
-
-
-
14
4
2
1
2
1
-
4
-
-
1
-
-
-
-
2
3
2
7
2
1
4
4
2
1
-
1
-
-
-
6
-
2
1
-
-
-
-
22
-
4
2
1
2
1
-
-
-
1
-
-
-
2
3
2
2
1
4
4
2
1
-
-
-
2
2
-
2
2
696915
Mouri
Design of a cytochrome P450BM3 ...
Escherichia coli
Biotechnol. Prog.
25
1372-1378
2009
-
-
-
-
-
-
-
-
1
-
1
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3
-
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1
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4
-
3
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1
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1
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1
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1
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4
-
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1
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-
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-
-
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-
-
672770
Sanchez
Effect of overexpression of a ...
Escherichia coli
Biotechnol. Prog.
22
420-425
2006
-
1
1
-
-
-
-
-
-
-
-
-
-
5
-
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-
-
-
-
-
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2
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1
1
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-
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-
668061
Ichinose
Enzymatic redox cofactor regen ...
Escherichia coli
Biotechnol. Prog.
21
1192-1197
2005
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
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1
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1
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-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
392593
Bykova
Two separate transhydrogenase ...
Pisum sativum, Solanum tuberosum
Biochem. Biophys. Res. Commun.
265
106-111
1999
-
-
-
-
-
-
-
-
4
-
-
-
-
12
-
-
-
-
-
2
2
-
2
-
-
-
-
-
-
-
-
-
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-
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-
-
-
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4
-
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-
2
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392592
French
Cloning, sequence, and propert ...
Pseudomonas fluorescens
J. Bacteriol.
179
2761-2765
1997
-
-
1
-
-
-
-
-
-
-
1
-
-
9
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
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-
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1
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-
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-
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1
-
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-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392574
Voordouw
Why are two different types of ...
Azotobacter vinelandii, Pseudomonas aeruginosa, Pseudomonas fluorescens
Eur. J. Biochem.
131
527-533
1983
-
-
-
-
-
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-
-
-
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3
-
-
24
-
-
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-
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3
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3
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-
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3
-
-
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-
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-
-
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-
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-
-
392575
Voordouw
Dissociation and assembly of p ...
Azotobacter vinelandii
Eur. J. Biochem.
127
267-274
1982
-
-
-
-
-
1
-
-
-
-
1
-
-
4
-
-
-
-
1
-
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-
1
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1
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-
1
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-
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1
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1
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1
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1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
392576
Voordouw
Pyridine nucleotide transhydro ...
Azotobacter vinelandii
Eur. J. Biochem.
111
347-355
1980
-
-
-
1
-
-
-
-
-
-
2
-
-
9
-
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1
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-
-
1
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-
2
-
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1
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2
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1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392577
Voordouw
Pyridine nucleotide transhydro ...
Azotobacter vinelandii
Eur. J. Biochem.
107
337-344
1980
-
-
-
-
-
-
4
-
-
2
1
-
-
4
-
-
-
-
-
-
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-
1
-
-
-
-
-
1
2
1
-
-
-
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-
-
-
-
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-
-
4
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
2
1
-
-
-
-
-
-
-
392578
Voordouw
Structure of pyridine nucleoti ...
Azotobacter vinelandii
Eur. J. Biochem.
98
447-454
1979
-
-
-
-
-
-
-
-
-
-
2
-
-
8
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
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-
-
2
-
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-
-
1
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392579
Höjeberg
Ca2+-dependent allosteric regu ...
Pseudomonas aeruginosa
Eur. J. Biochem.
77
235-241
1977
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
392580
Collins
Transhydrogenase activity in t ...
Vibrio natriegens
Biochim. Biophys. Acta
480
77-82
1977
-
-
-
-
-
-
12
-
-
-
-
-
-
11
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
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-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
392581
Widmer
Regulatory properties of the p ...
Pseudomonas aeruginosa
Biochemistry
15
4693-4699
1976
1
-
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1
1
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6
-
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1
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2
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1
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-
392582
Wermuth
Pyridine nucleotide transhydro ...
Pseudomonas aeruginosa
Arch. Biochem. Biophys.
176
136-143
1976
1
-
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-
-
1
-
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-
1
3
-
-
3
-
-
1
-
1
-
1
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1
-
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-
-
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-
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1
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-
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1
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1
3
-
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1
1
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1
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-
1
-
-
-
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-
-
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-
392595
Rydström
-
Nicotinamide nucleotide transh ...
Azotobacter vinelandii, Azotobacter agilis, Azotobacter chroococcum, Chromatium sp., Pseudomonas aeruginosa, Pseudomonas fluorescens
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
51-88
1976
6
-
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4
-
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5
-
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6
-
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1
3
-
-
4
-
13
-
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-
-
4
-
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5
-
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6
-
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5
-
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4
-
-
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5
-
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1
-
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4
-
13
-
-
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4
-
-
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-
-
-
-
-
392583
Hoek
Comparative studies on nicotin ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
333
237-245
1974
1
-
-
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1
-
-
1
-
-
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11
-
-
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-
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3
-
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1
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1
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1
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3
-
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-
-
-
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-
-
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-
-
-
-
392584
Rydström
Ca 2+ -dependent allosteric re ...
Pseudomonas aeruginosa
Biochem. Biophys. Res. Commun.
52
421-429
1973
2
-
-
-
-
-
1
-
-
4
-
1
-
3
-
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-
-
-
-
-
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2
-
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-
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2
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2
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4
-
1
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2
-
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-
-
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-
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-
392585
Middleditch
Pyridine nucleotide transhydro ...
Azotobacter vinelandii
J. Biol. Chem.
247
6802-6809
1972
-
-
-
-
-
-
-
-
-
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2
-
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3
-
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1
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1
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2
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1
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1
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2
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1
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1
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2
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-
-
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-
392586
Van den Broek
Pyridine-nucleotide transhydro ...
Azotobacter vinelandii
Eur. J. Biochem.
24
31-45
1971
1
-
-
-
-
2
1
2
-
1
1
-
-
5
-
-
1
-
-
-
1
1
7
-
-
-
-
2
-
-
-
-
1
-
-
-
1
-
-
1
-
-
2
-
1
-
2
-
1
1
-
-
-
-
1
-
-
1
1
7
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
392587
Van den Broek
Pyridine-nucleotide transhydro ...
Azotobacter vinelandii
Eur. J. Biochem.
24
46-54
1971
-
-
-
1
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392588
Van den Broek
Pyridine-nucleotide transhydro ...
Azotobacter vinelandii
Eur. J. Biochem.
24
72-82
1971
-
-
-
-
-
-
-
10
-
-
-
-
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5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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392589
Cohen
Kinetic characteristics of the ...
Pseudomonas aeruginosa
J. Biol. Chem.
245
4666-4672
1970
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-
-
-
-
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1
-
-
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3
-
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-
-
-
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-
1
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392590
Cohen
Purification and properties of ...
Pseudomonas aeruginosa
J. Biol. Chem.
245
2825-2836
1970
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-
-
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1
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3
-
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1
-
-
-
-
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5
-
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-
-
1
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
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1
-
-
-
-
5
-
-
-
1
1
-
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392591
Chung
Pyridine nucleotide transhydro ...
Azotobacter vinelandii
J. Bacteriol.
102
438-447
1970
1
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2
5
-
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3
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-
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-
1
-
3
-
-
1
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1
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-
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-
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2
-
5
-
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1
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3
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1
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