EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.1.1.145 | 37 |
- |
30 min, 50% loss of activity |
389372 |
1.1.1.145 | 37 |
- |
3betaHSD2 becomes less stable when incubated with the individual lipids dipalmitoylphosphatidylglycerol or dipalmitoylphosphatidylcholine, as indicated by the decrease in thermal denaturation (Tm) from 42 to 37°C. Dipalmitoylphosphatidylglycerol alone or in combination with dipalmitoylphosphatidylcholine, leads to a decrease in alpha-helical content without an effect on the beta-sheet conformation |
724302 |
1.1.1.145 | 37 |
- |
approximately, melting temperature in the presence of zwitterionically charged dipalmitoylphosphatidylcholine vesicles or an equal mixture of zwitterionic vesicles and anionic vesicles |
724302 |
1.1.1.145 | 42 |
- |
melting temperature in the absence of lipid |
724302 |
1.1.1.145 | 42 |
- |
Tm in the absence of any lipid |
724302 |
1.1.1.145 | 55 |
- |
50 min, in microsomes 40-55% loss of activity, solubilized 80% loss of activity |
389373 |
1.1.1.145 | 60 |
- |
microsomal fraction, inactivation |
389373 |
1.1.1.145 | 65 |
- |
complete denaturation |
724302 |