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(S)-1-indanol + NADP+
? + NADPH
-
-
-
-
r
(S)-1-tetralol + NADP+
? + NADPH
-
-
-
-
r
(S)-phenyl-1-butanol + NADP+
? + NADPH
-
-
-
-
r
1,4-naphthoquinone + NADPH
? + NADP+
-
-
-
-
r
1-phenylisatin + NADPH
? + NADP+
-
-
-
-
r
11beta,17alpha,21-trihydroxy-4-pregnen-3,20-dione + NADPH
3beta,11beta,17alpha,21-tetrahydroxy-4-pregnen-20-one + NADP+
-
-
-
-
?
11beta,17alpha,21-trihydroxy-5alpha-pregnane-3,20-dione + NADPH
3beta,11beta,17alpha,21-tetrahydroxy-5alpha-pregnan-20-one + NADP+
-
-
-
-
?
11beta,17alpha,21-trihydroxy-5beta-pregnane-3,20-dione + NADPH
3beta,11beta,17alpha,21-tetrahydroxy-5beta-pregnan-20-one + NADP+
-
-
-
-
?
13,14-dihydro-15-keto-prostaglandin F2 + NADPH
?
-
-
-
-
?
13,14-dihydro-15-keto-prostaglandin F2alpha + NADPH
?
-
-
-
-
?
16alpha-hydroxy-dehydroepiandrosterone + NAD+
?
-
-
-
?
16alpha-hydroxy-dehydroepiandrosterone + NAD+
? + NADH
-
-
-
?
16beta-hydroxy-dehydroepiandrosterone + NAD+
?
-
-
-
?
16beta-hydroxy-dehydroepiandrosterone + NAD+
? + NADH
-
-
-
?
17-hydroxyprogesterone + NADPH
3beta,17-dihydroxy-4-pregnen-20-one + NADP+
-
-
-
-
?
17alpha-hydroxypregnenolone + NAD+
17alpha-hydroxy-pregn-5-en-3,20-dione + NADH
17beta-hydroxy-5alpha-androstane-3-one + NADPH
3beta,17beta-dihydroxy-5alpha-androstane + NADP+
-
-
-
-
?
17beta-hydroxy-5beta-androstane-3-one + NADPH
3beta,17beta-dihydroxy-5beta-androstane + NADP+
-
-
-
-
?
2,3-heptanedione + NADPH
? + NADP+
-
-
-
-
r
2,3-hexanedione + NADPH
? + NADP+
-
-
-
-
r
2,3-pentanedione + NADPH + H+
? + NADP+
-
-
-
-
r
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
3beta,7alpha-dihydroxy-5-cholestenoic acid + NAD+
7alpha-hydroxy-3-oxo-5-cholestenoic acid + NADH
-
-
-
-
?
3beta-hydroxy-5-androsten-17-one + NAD+
androstendione + NADH + H+
3beta-hydroxyandrostan-17-one + NAD+
androstan-3,17-dione + NADH
-
-
-
-
?
4,4'-dimethylbenzil + NADPH
? + NADP+
-
-
-
-
r
4-androsten-17beta-ol-3-one + NADH + H+
4-androsten-3beta,17beta-diol + NAD+
-
-
-
r
4-androsten-3beta,17beta-diol + NAD+
4-androsten-17beta-ol-3-one + NADH
-
-
-
-
?
4-benzoylpyridine + NADPH
? + NADP+
-
-
-
-
r
4-benzylpyridine + NADPH
?
-
-
-
-
?
4-carboxybenzaldehyde + NADP+ + H2O
4-carboxybenzoic acid + NADPH + H+
-
-
-
-
?
4-hexanoylpyridine + NADPH
? + NADP+
-
-
-
-
r
4-nitroacetophenone + NADPH
1-(4-nitrophenyl)ethanol + NADP+
-
-
-
-
?
4-nitrobenzaldehyde + NADPH
4-nitrobenzyl alcohol + NADP+
-
-
-
-
?
4-nitrobenzaldehyde + NADPH
? + NADP+
-
-
-
-
r
5,16-androstadien-3beta-ol + NAD+
5,16-androstadien-3-one + NADH
-
-
-
-
?
5-androsten-3beta-ol-17-one + NAD+
5-androsten-3,17-dione + NADH
-
-
-
r
5-pregnen-3beta-ol-2-one + NAD+
progesterone + NADH
5alpha-androstan-3beta-ol-17-one + NADP+
5alpha-androstan-3,17-dione + NADPH
5alpha-androstane-3,17-dione + NADPH
3beta-hydroxy-5alpha-androstane-17-one + NADP+
-
-
-
-
?
5alpha-androstane-3,17-dione + NADPH
5alpha-androstane-3beta-ol-17-one + NADP+
-
-
-
-
r
5alpha-androstane-3beta,17beta-diol + NADP+
5alpha-androstane-17beta-ol-3-one + NADPH
-
-
-
-
r
5alpha-androstanediol + NAD+
5alpha-dihydrotestosterone + NADH
-
-
-
-
r
5alpha-dihydrotestosterone + NADPH
5alpha-androstane-3beta,17beta-diol + NADP+
-
-
-
-
?
5alpha-dihydrotestosterone + NADPH
? + NADP+
-
-
-
-
r
5alpha-pregnan-3beta-ol-20-one + NAD+
5alpha-pregnan-3,20-dione + NADH
-
-
-
-
?
5alpha-pregnane-3,20-dione + NAD(P)H
5alpha-pregnane-3beta-ol-20-one + NAD(P)+
5alpha-pregnane-3,20-dione + NADH
5alpha-pregnane-3beta-ol-20-one + NAD+
-
-
-
r
5alpha-pregnane-3beta-ol-20-one + NAD+
5alpha-pregnane-3,20-dione + NADH
-
-
-
r
5alpha-pregnane-3beta-ol-20-one + NADP+
5alpha-pregnane-3,20-dione + NADPH
-
-
-
-
r
5beta-androstan-17beta-ol-3-one + NADPH
5beta-androstane-3beta,17beta-diol + NADP+
-
-
-
-
r
5beta-androstan-3beta-ol-17-one + NADP+
5beta-androstane-3,17-dione + NADPH
-
-
-
-
r
5beta-androstane-3,17-dione + NADPH
3beta-hydroxy-5beta-androstane-17-one + NADP+
-
-
-
-
?
5beta-androstane-3,17-dione + NADPH
5beta-androstan-3beta-ol-17-one + NADP+
-
-
-
-
r
5beta-androstane-3beta,17beta-diol + NADP+
5beta-androstan-17beta-ol-3-one + NADPH
-
-
-
-
r
5beta-pregnan-20alpha-ol-3-one + NADPH + H+
5beta-pregnan-3beta,20alpha-diol + NADP+
-
-
-
-
r
5beta-pregnan-3beta-ol-20-one + NADP+
5beta-pregnan-3,20-dione + NADPH
-
-
-
-
r
5beta-pregnane-3,20-dione + NAD(P)H + H+
5beta-pregnane-3beta-ol-20-one + NAD(P)+
5beta-pregnane-3,20-dione + NADH + H+
5beta-pregnane-3beta-ol-20-one + 5beta-pregnane-3alpha-ol-20-one + NAD+
-
-
-
r
5beta-pregnane-3,20-dione + NADPH + H+
5beta-pregnan-3beta-ol-20-one + NADP+
-
-
-
-
r
5beta-pregnane-3beta,20alpha-diol + NADP+
5beta-pregnane-20alpha-ol-3-one + NADPH + H+
-
-
-
-
r
5beta-pregnane-3beta-ol-20-one + NAD+
5beta-pregnane-3,20-dione + NADH + H+
-
-
-
r
6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione + NADPH
? + NADP+
-
major product has S-configuration
-
-
r
7alpha,25-dihydroxycholesterol + NAD+
7alpha,25-dihydroxy-4-cholesten-3-one + NADH
-
-
-
-
?
7alpha,27-dihydroxycholesterol + NAD+
7alpha,27-dihydroxy-4-cholesten-3-one + NADH
-
-
-
-
?
7alpha-hydroxycholesterol + NAD+
7alpha-hydroxy-4-cholesten-3-one + NADH
-
-
-
-
?
9,10-phenanthrenequinone + NADPH
?
-
-
-
-
?
9,10-phenanthrenequinone + NADPH
? + NADP+
all-trans-retinal + NADPH
? + NADP+
-
-
-
-
r
all-trans-retinol + NADP+
? + NADPH
-
-
-
-
r
allopregnanolone + NAD+
?
-
-
-
-
r
androst-5-en-3,17-dione + NADH
dehydroepiandrosterone + NAD+
-
-
-
r
androst-5-ene-3beta-ol-17-one + NAD+
androst-5-ene-3,17-dione + NADH
androstanediol + NAD+
dihydrotestosterone + NADH + H+
-
-
-
-
r
androstendiol + NAD+
testosterone + NADH + H+
androstenediol + NAD+
testosterone + NADH + H+
-
-
-
-
r
androstenedione + NADPH + H+
3beta-hydroxy-5-androsten-17-one + NADP+
-
-
-
-
?
androstenone + NAD+
5alpha-androst-16-en-3beta-ol + NADH + H+
-
-
-
-
?
androstenone + NADH
beta-androstenol + NAD+
-
-
-
?
benzil + NADPH
(R)-benzoin + NADP+
-
enantiomeric purity of 97% enantiomeric excess
-
-
r
camphorquinone + NADPH + H+
camphorquinol + NADP+
-
-
-
-
?
caprinaldehyde + NADPH
?
-
-
-
-
?
cholesterol + NAD+
cholest-5-en-3-one + NADH
-
-
-
-
?
cyclohexanone + NADPH
cyclohexanol + NADP+
-
-
-
-
?
D-galactose + NADPH
?
-
-
-
-
?
D-glucose + NADPH
?
-
-
-
-
?
D-glucuronic acid + NADPH
?
-
-
-
-
?
D-glyceraldehyde + NADPH
?
-
-
-
-
?
D-xylose + NADPH
?
-
-
-
-
?
dehydroepiandrosterone + NAD+
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH + H+
dehydroepiandrosterone + NADH + H+
androsterone + NAD+
-
-
-
-
?
dehydroepiandrosterone + NADPH
3beta-hydroxy-5-androsten-17-one + NADP+
-
-
-
-
?
dehydrolithocholic acid + NADPH
isolithocholic acid + NADP+
-
-
-
-
r
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
DELTA5-androstenediol + NAD+
testosterone + NADH + H+
-
-
-
-
?
diacetyl + NADPH
? + NADP+
-
-
-
-
r
dimethyl-2-oxo-glutarate + NADPH
? + NADP+
-
-
-
-
r
epiandrosterone + NAD+
5alpha-androstan-3,17-dione + NADH + H+
-
-
-
-
r
hexanophenone + NADPH
? + NADP+
-
-
-
-
r
hydritantin + NADPH
?
-
-
-
-
?
isatin + NADPH
? + NADP+
-
-
-
-
r
isolithocholic acid + NADP+
dehydrolithocholic acid + NADPH
-
-
-
-
r
menadione + NADPH
? + NADP+
-
-
-
-
r
menadione + NADPH
menadiol + NADP+
-
-
-
-
?
methylglyoxal + NADPH
?
-
-
-
-
?
n-butyrophenone + NADPH
(S)-1-phenyl1-butanol + NADP+
-
major product
-
-
r
phenylglyoxal + NADPH
?
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
pregnenolone + NAD+
isoprogesterone + NADH + H+
pregnenolone + NAD+
isoprogesterone + progesterone + NADH
-
about 90% odf product is isoprogesterone
-
r
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
pregnenolone + NAD+
progesterone + NADH
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
progesterone + NADPH
3beta-hydroxy-4-pregnen-20-one + NADP+
-
-
-
-
?
prostaglandin E1 + NADPH
?
-
-
-
-
?
prostaglandin E2 + NADPH
?
-
-
-
-
?
pyridine-4-aldehyde + NADPH
? + NADP+
-
-
-
-
r
sitosterol + NAD+
3-oxo-24-ethyl-cholest-5-ene + NADH + H+
testosterone + NADPH
? + NADP+
-
-
-
-
r
testosterone + NADPH + H+
3beta,17beta-dihydroxy-4-androsten + NADP+
-
-
-
-
?
valerophenone + NADPH
? + NADP+
-
-
-
-
r
additional information
?
-
17alpha-hydroxypregnenolone + NAD+

17alpha-hydroxy-pregn-5-en-3,20-dione + NADH
-
-
-
?
17alpha-hydroxypregnenolone + NAD+
17alpha-hydroxy-pregn-5-en-3,20-dione + NADH
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+

17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta-hydroxy-5-androsten-17-one + NAD+

androstendione + NADH + H+
-
-
-
-
?
3beta-hydroxy-5-androsten-17-one + NAD+
androstendione + NADH + H+
-
-
-
-
?
3beta-hydroxy-5-androsten-17-one + NAD+
androstendione + NADH + H+
-
-
-
-
?
5-pregnen-3beta-ol-2-one + NAD+

progesterone + NADH
-
-
-
-
?
5-pregnen-3beta-ol-2-one + NAD+
progesterone + NADH
-
trivial name pregnenolone
-
-
?
5alpha-androstan-3beta-ol-17-one + NADP+

5alpha-androstan-3,17-dione + NADPH
-
-
-
-
?
5alpha-androstan-3beta-ol-17-one + NADP+
5alpha-androstan-3,17-dione + NADPH
-
-
-
-
r
5alpha-pregnane-3,20-dione + NAD(P)H

5alpha-pregnane-3beta-ol-20-one + NAD(P)+
-
-
-
-
r
5alpha-pregnane-3,20-dione + NAD(P)H
5alpha-pregnane-3beta-ol-20-one + NAD(P)+
-
-
-
-
r
5alpha-pregnane-3,20-dione + NAD(P)H
5alpha-pregnane-3beta-ol-20-one + NAD(P)+
-
-
-
-
?
5beta-pregnane-3,20-dione + NAD(P)H + H+

5beta-pregnane-3beta-ol-20-one + NAD(P)+
-
-
-
-
r
5beta-pregnane-3,20-dione + NAD(P)H + H+
5beta-pregnane-3beta-ol-20-one + NAD(P)+
-
-
-
-
r
5beta-pregnane-3,20-dione + NAD(P)H + H+
5beta-pregnane-3beta-ol-20-one + NAD(P)+
-
-
-
-
?
9,10-phenanthrenequinone + NADPH

? + NADP+
-
-
-
-
r
9,10-phenanthrenequinone + NADPH
? + NADP+
-
-
-
-
?
androst-5-ene-3beta-ol-17-one + NAD+

androst-5-ene-3,17-dione + NADH
-
-
-
-
ir
androst-5-ene-3beta-ol-17-one + NAD+
androst-5-ene-3,17-dione + NADH
-
-
-
-
ir
androstendiol + NAD+

testosterone + NADH + H+
-
-
-
-
?
androstendiol + NAD+
testosterone + NADH + H+
-
-
-
-
?
androstendiol + NAD+
testosterone + NADH + H+
-
-
-
-
?
dehydroepiandrosterone + NAD+

?
-
-
-
-
?
dehydroepiandrosterone + NAD+
?
-
-
-
r
dehydroepiandrosterone + NAD+
?
-
-
-
?
dehydroepiandrosterone + NAD+
?
isozyme type 1
-
-
?
dehydroepiandrosterone + NAD+
?
-
-
-
-
r
dehydroepiandrosterone + NAD+
?
-
-
-
-
r
dehydroepiandrosterone + NAD+
?
-
-
-
-
r
dehydroepiandrosterone + NAD+

androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
r
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+

androst-5-en-3,17-dione + NADH + H+
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH + H+
-
-
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+

DELTA4-3-oxosteroid + NADH + H+
-
the enzyme is essential for biosynthesis of all classes of steroid hormones, molecular mechanisms of enzyme regulation involving the Nur77 family of immediate-early orphan nuclear receptors, e.g. Nur77 targets the promoter of gene HSD3B2 in testicular Leygig cells, overview
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
-
the enzyme performs the 3beta-hydroxysteroid dehydrogenase and DELTA5-DELTA4 isomerase reactions
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
the enzyme is essential for biosynthesis of all classes of steroid hormones
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
the enzyme performs the 3beta-hydroxysteroid dehydrogenase and DELTA5-DELTA4 isomerase reactions
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
the enzyme is essential for biosynthesis of all classes of steroid hormones
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
the enzyme performs the 3beta-hydroxysteroid dehydrogenase and DELTA5-DELTA4 isomerase reactions
-
-
?
pregnenolone + NAD(P)+

pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
ir
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
ir
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD+

isoprogesterone + NADH + H+
-
-
-
?
pregnenolone + NAD+
isoprogesterone + NADH + H+
-
-
-
?
pregnenolone + NAD+

pregn-5-en-3,20-dione + NADH
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH
-
-
-
-
?
pregnenolone + NAD+

pregn-5-en-3,20-dione + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
-
-
-
-
?
pregnenolone + NAD+

progesterone + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
isozyme type 2
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
-
-
-
-
?
sitosterol + NAD+

3-oxo-24-ethyl-cholest-5-ene + NADH + H+
-
the enzyme is involved in the catabolic pathway for the degradation of sitosterol to androstenedione
-
-
?
sitosterol + NAD+
3-oxo-24-ethyl-cholest-5-ene + NADH + H+
-
the enzyme is involved in the catabolic pathway for the degradation of sitosterol to androstenedione
-
-
?
additional information

?
-
-
no reaction with OH in 3alpha, 17beta, 11beta, 21 position
-
-
?
additional information
?
-
-
the enzyme is regulated on expression not on catalytic activity level
-
-
?
additional information
?
-
-
trifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, and of the 17beta-hydroxysteroid dehydrogenase type II, EC 1.1.1.51
-
-
?
additional information
?
-
no substrate: cholesterol
-
-
?
additional information
?
-
enzyme catalyzes at least two reactions in the cardenolide pathway, i.e. dehydrogenation of pregnenolone to isoprogesterone and the reduction of 5beta-pregnane-3,20-dione to 5beta-pregnan-3beta-ol-20-one. Recombinant enzyme displays 3alpha-, 3beta- and 17beta-dehydrogenase as well as 3/17-keto reductase activities
-
-
?
additional information
?
-
enzyme catalyzes at least two reactions in the cardenolide pathway, i.e. dehydrogenation of pregnenolone to isoprogesterone and the reduction of 5beta-pregnane-3,20-dione to 5beta-pregnan-3beta-ol-20-one. Recombinant enzyme displays 3alpha-, 3beta- and 17beta-dehydrogenase as well as 3/17-keto reductase activities
-
-
?
additional information
?
-
the enzyme is essential for biosynthesis of all classes of steroid hormones catalyzing the first step of the multienzyme pathway conversion of circulating dehydroepiandrosterone and pregnenolone to active steroid hormones
-
-
?
additional information
?
-
-
the enzyme is essential for biosynthesis of all classes of steroid hormones catalyzing the first step of the multienzyme pathway conversion of circulating dehydroepiandrosterone and pregnenolone to active steroid hormones
-
-
?
additional information
?
-
-
the enzyme is essential for biosynthesis of all classes of steroid hormones, pathway overview
-
-
?
additional information
?
-
the enzyme is essential for biosynthesis of all classes of steroid hormones, pathway overview
-
-
?
additional information
?
-
the enzyme is essential for biosynthesis of all classes of steroid hormones, pathway overview
-
-
?
additional information
?
-
-
the enzyme is involved in steroid production and/or metabolism
-
-
?
additional information
?
-
-
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
-
-
?
additional information
?
-
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
-
-
?
additional information
?
-
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
-
-
?
additional information
?
-
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
-
-
?
additional information
?
-
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
-
-
?
additional information
?
-
-
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
-
-
?
additional information
?
-
-
enzyme exhibits high stereo-selectivity yielding 3-beta-hydroxysteroids from 3-ketosteroids
-
-
?
additional information
?
-
using fluorescence resonance energy transfer, it is shown that 3betaHSD2 interacts strongly with a mixture of dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine
-
-
?
additional information
?
-
enzyme diplays lipid-mediated unfolding. As isoform 3betaHSD2 lacks a receptor, opening the conformation may activate the protein by forming a cavity forms between the alpha-helix and beta-sheet
-
-
?
additional information
?
-
metabolic conversion assays indicate peak 3betaHSD2 activity at pH 4.5 with 2fold more progesterone synthesized at pH 4.5 than at pH 3.5 and 7.4. Increasing the 3betaSD2 concentration from 1 to 40 microg results in a 7fold increase in progesterone at pH 4.5, but no change at pH 7.4
-
-
?
additional information
?
-
-
no reaction with OH in 17alpha, 17beta, 20alpha, 20beta position
-
-
?
additional information
?
-
-
enzyme regulation by steroids, overview
-
-
?
additional information
?
-
-
specific for 3beta-OH group in steroids with trans configuration of A/B rings and DELTA5-double bond
-
-
?
additional information
?
-
relationship between the expression of hepatic but not testicular enzyme with androstenone deposition in pig adipose tissue, overview
-
-
?
additional information
?
-
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
17alpha-hydroxypregnenolone + NAD+
17alpha-hydroxy-pregn-5-en-3,20-dione + NADH
5-pregnen-3beta-ol-2-one + NAD+
progesterone + NADH
androstenone + NAD+
5alpha-androst-16-en-3beta-ol + NADH + H+
-
-
-
-
?
androstenone + NADH
beta-androstenol + NAD+
-
-
-
?
dehydroepiandrosterone + NAD+
?
isozyme type 1
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
dehydroepiandrosterone + NADH + H+
androsterone + NAD+
-
-
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
DELTA5-androstenediol + NAD+
testosterone + NADH + H+
-
-
-
-
?
epiandrosterone + NAD+
5alpha-androstan-3,17-dione + NADH + H+
-
-
-
-
r
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
pregnenolone + NAD+
progesterone + NADH + H+
additional information
?
-
17alpha-hydroxypregnenolone + NAD+

17alpha-hydroxy-pregn-5-en-3,20-dione + NADH
-
-
-
?
17alpha-hydroxypregnenolone + NAD+
17alpha-hydroxy-pregn-5-en-3,20-dione + NADH
-
-
-
-
?
5-pregnen-3beta-ol-2-one + NAD+

progesterone + NADH
-
-
-
-
?
5-pregnen-3beta-ol-2-one + NAD+
progesterone + NADH
-
trivial name pregnenolone
-
-
?
dehydroepiandrosterone + NAD+

androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH
-
-
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+

DELTA4-3-oxosteroid + NADH + H+
-
the enzyme is essential for biosynthesis of all classes of steroid hormones, molecular mechanisms of enzyme regulation involving the Nur77 family of immediate-early orphan nuclear receptors, e.g. Nur77 targets the promoter of gene HSD3B2 in testicular Leygig cells, overview
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
the enzyme is essential for biosynthesis of all classes of steroid hormones
-
-
?
DELTA5-3beta-hydroxysteroid + NAD+
DELTA4-3-oxosteroid + NADH + H+
the enzyme is essential for biosynthesis of all classes of steroid hormones
-
-
?
pregnenolone + NAD(P)+

pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
ir
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
ir
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H
-
-
-
-
?
pregnenolone + NAD+

pregn-5-en-3,20-dione + NADH
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH
-
-
-
-
?
pregnenolone + NAD+

pregn-5-en-3,20-dione + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
-
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
-
-
-
-
?
pregnenolone + NAD+

progesterone + NADH + H+
isozyme type 2
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
-
-
-
-
?
additional information

?
-
-
the enzyme is regulated on expression not on catalytic activity level
-
-
?
additional information
?
-
the enzyme is essential for biosynthesis of all classes of steroid hormones catalyzing the first step of the multienzyme pathway conversion of circulating dehydroepiandrosterone and pregnenolone to active steroid hormones
-
-
?
additional information
?
-
-
the enzyme is essential for biosynthesis of all classes of steroid hormones catalyzing the first step of the multienzyme pathway conversion of circulating dehydroepiandrosterone and pregnenolone to active steroid hormones
-
-
?
additional information
?
-
-
the enzyme is essential for biosynthesis of all classes of steroid hormones, pathway overview
-
-
?
additional information
?
-
the enzyme is essential for biosynthesis of all classes of steroid hormones, pathway overview
-
-
?
additional information
?
-
the enzyme is essential for biosynthesis of all classes of steroid hormones, pathway overview
-
-
?
additional information
?
-
-
the enzyme is involved in steroid production and/or metabolism
-
-
?
additional information
?
-
using fluorescence resonance energy transfer, it is shown that 3betaHSD2 interacts strongly with a mixture of dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine
-
-
?
additional information
?
-
-
enzyme regulation by steroids, overview
-
-
?
additional information
?
-
relationship between the expression of hepatic but not testicular enzyme with androstenone deposition in pig adipose tissue, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(12S,12aS,14bR)-N,N,12a,14b-tetramethyl-3-oxo-1,2,3,4,5,6,7,9,9a,9b,10,11,12,12a,13,14,14a,14b-octadecahydroazepino[3,2,1-de]cyclopenta[i]phenanthridine-12-carboxamide
-
(1R,4S,9aR,11aR)-4,9a,11a-trimethyl-1-((R)-6-methylheptan-2-yl)-3,3a,3b,4,5,8,9,9a,9b,10,11,11a-dodecahydro-1H-cyclopenta[i]phenanthridin-7(2H)-one
-
(1R,9aR,11aR)-9a,11a-dimethyl-1-((R)-6-methylheptan-2-yl)-3,3a,3b,4,5,8,9,9a,9b,10,11,11a-dodecahydro-1H-cyclopenta[i]phenanthridin-7(2H)-one
-
(1S,8bS,10aS)-N,N-diethyl-8b,10a-dimethyl-7-oxo-1,2,3,3a,3b,4,5,7,7a,8,8a,8b,8c,9,10,10a-hexadecahydrocyclopenta[i]cyclopropa[a]phenanthridine-1-carboxamide
-
(1S,8bS,10aS)-N-tert-butyl-8b,10a-dimethyl-7-oxo-1,2,3,3a,3b,4,5,7,7a,8,8a,8b,8c,9,10,10a-hexadecahydrocyclopenta[i]cyclopropa[a]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-1-(4,5-diphenyl-4,5-dihydro-1,3-oxazol-2-yl)-9a,11a-dimethyl-1,2,3,3a,3b,4,5,8,9,9a,9b,10,11,11a-tetradecahydro-7H-cyclopenta[i]phenanthridin-7-one
-
(1S,9aR,11aS)-5-butyl-N,N,9a,11a-tetramethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-5-hexyl-N,N,9a,11a-tetramethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-6-ethyl-N,N,9a,11a-tetramethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-9a,11a-dimethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxylic acid
-
(1S,9aR,11aS)-9a,11a-dimethyl-N-[4-(morpholin-4-yl)phenyl]-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-N,N,5,9a,11a-pentamethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-N,N,6,9a,11a-pentamethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-N,N,9a,11a-tetramethyl-7-oxo-5-propyl-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-N-(2,5-di-tert-butylphenyl)-9a,11a-dimethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-N-(2-benzoylphenyl)-9a,11a-dimethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-N-tert-butyl-9a,11a-dimethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(1S,9aR,11aS)-N-[2-tert-butyl-5-(trifluoromethyl)phenyl]-9a,11a-dimethyl-7-oxo-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-cyclopenta[i]phenanthridine-1-carboxamide
-
(4aR,6aS,7S)-7-[2,5-bis(trifluoromethyl)benzoyl]-4a,6a-dimethyl-1,4a,4b,5,6,6a,7,8,9,9a,9b,10,11,11a-tetradecahydro-2H-indeno[5,4-f]quinolin-2-one
-
(4aR,6aS,7S)-N-tert-butyl-4a,6a-dimethyl-2-oxo-2,4a,4b,5,6,6a,7,8,9,9a,9b,10,11,11a-tetradecahydro-1H-indeno[5,4-f]quinoline-7-carboxamide
-
17alpha-hydroxypregnenolone
-
competitive inhibition
17beta-acetoxy-trilostane
-
-
20alpha-hydroxy-4-pregnen-3-one
-
-
20beta-hydroxy-4-pregnen-3-one
-
-
3-Chloroacetylpyridine adenine dinucleotide
-
-
4-nonylphenol
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
4alpha,5alpha-epoxy-testosterone
-
-
5'-fluorosulfonylbenzoyl adenosine
-
-
5,10-Secoestr-4-yne-3,10,17-trione
-
irreversible
6-hydroxyflavone
-
0.00056 mM, 50% inhibition; competitive
Cetylpyridinium chloride
-
-
corticosterone
-
0.08 mM, 50% inhibition
cyanoketone
-
0.0000076 mM, 50% inhibition; competitive
dehydroepiandrosterone
-
0.01 mM reduced conversion of pregnenolone to 50%
diethylhexyl phthalate
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
diethylstilbestrol
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
estradiol
-
0.0005 mM, 40% inhibition, 0.001 mM, 62% inhibition
Fe2+
-
1 mM: weak, 100 mM: strong
flavanones
-
diverse derivatives, inhibitory potential determination, overview
flavones
-
diverse derivatives, inhibitory potential determination, overview
isoflavones
-
diverse derivatives, inhibitory potential determination, overview
ketoconazole
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
lauric acid
-
0.05 mM, less than 10% inhibition
monooctyltin
-
0.081 mM, 50% inhibition; inhibition mechanism, overview
myricetin
-
0.011 mM, 50% inhibition
myristic acid
-
0.05 mM, less than 10% inhibition
N-(Anilino-naphthyl-4)-maleimide
-
-
N-Iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylene diamine
-
-
NADH
-
mixed type inhibition
octylphenol
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
oestradiol
-
0.00024 mM, 50% inhibition
pregnenolone
-
competitive inhibition
tributyltin
-
0.012 mM, almost complete loss of activity; complete inhibition at 0.012 mM, the inhibition mechanism is not entirely competitive, overview
(+)-gossypol

-
potent inhibitor
(+)-gossypol
-
potent inhibitor
(-)-gossypol

-
potent inhibitor
(-)-gossypol
-
potent inhibitor
apigenin

-
-
apigenin
-
0.13 mM, 50% inhibition
Biochanin A

-
-
Biochanin A
-
0.00094 mM, 50% inhibition
Cd2+

-
1 mM: strong
Co2+

-
1 mM: weak, 100 mM: strong
Cu2+

-
1 mM: strong
daidzein

-
-
daidzein
-
0.0033 mM, 50% inhibition
epostane

-
-
epostane
inhibits the 3beta-HSD activity of the wild-type 1 isozyme with 14â17fold greater affinity compared to the wild-type 2 isozyme and mutant H156Y; inhibits the 3beta-HSD activity of the wild-type 1 isozyme with 14â17fold greater affinity compared to the wild-type 2 isozyme and mutant H156Y
epostane
-
competitive inhibition
epostane
inhibits the dehydroepiandrosterone-induced proliferation of MCF-7 cells expressing isoform 3beta-HSD1 with 12- to 16fold lower IC50 values compared to the MCF-7 cells expressing isoform 3beta-HSD2. Epostane also competitively inhibits purified human 3beta-HSD1 with 12fold to 16fold lower Ki values compared to the noncompetitive Ki values measured for human 3beta-HSD2; inhibits the dehydroepiandrosterone-induced proliferation of MCF-7 cells expressing isoform 3beta-HSD1 with 12- to 16fold lower IC50 values compared to the MCF-7 cells expressing isoform 3beta-HSD. Epostane also competitively inhibits purified human 3beta-HSD1 with 12fold to 16fold lower Ki values compared to the noncompetitive Ki values measured for human 3beta-HSD2
ethynylestradiol

-
-
formononetin

-
-
formononetin
-
0.0032 mM, 50% inhibition
genistein

-
-
genistein
-
0.00026 mM, 50% inhibition; competitive
Ni2+

-
1 mM: weak, 100 mM: strong
Norethisterone

-
-
Norethisterone acetate

-
-
Norethisterone acetate
-
-
p-chloromercuribenzoate

-
-
p-chloromercuribenzoate
-
-
progesterone

-
-
progesterone
-
0.026 mM, 50% inhibition
progesterone
-
0.025 mM, 44% inhibition, 0.05 mM, 67% inhibition
quercetin

-
0.084 mM, 50% inhibition
testosterone

-
0.0086 mM, 50% inhibition
trilostane

-
i.e. 4alpha,5-epoxy-3,17beta-dihydroxy-5alpha-androst-2-en-2-carbonitrile
trilostane
-
i.e. 2alpha-cyano-4alpha,5alpha-epoxy-17beta-ol-androstane-3-one
trilostane
-
i.e. 2alpha-cyano-4alpha,5alpha-epoxy-17beta-ol-androstane-3-one, competitive inhibition
trilostane
-
human adrenal H295R cells treated with the HSD3B2 inhibitor, trilostane, demonstrate a markedly decreased androstenedione production
trilostane
inhibits the dehydroepiandrosterone-induced proliferation of MCF-7 cells expressing isoform 3beta-HSD1 with 12- to 16fold lower IC50 values compared to the MCF-7 cells expressing isoform 3beta-HSD2. Trilostane also competitively inhibits purified human 3beta-HSD1 with 12fold to 16fold lower Ki values compared to the noncompetitive Ki values measured for human 3beta-HSD2; inhibits the dehydroepiandrosterone-induced proliferation of MCF-7 cells expressing isoform 3beta-HSD1 with 12- to 16fold lower IC50 values compared to the MCF-7 cells expressing isoform 3beta-HSD2. Trilostane also competitively inhibits purified human 3beta-HSD1 with 12fold to 16fold lower Ki values compared to the noncompetitive Ki values measured for human 3beta-HSD2. Trilostane is docked in the active site of isoform 3beta-HSD1, and residue Arg195 in 3beta-HSD1 is a potentially critical residue
trilostane
-
0.004 mM, 50% inhibition
trilostane
-
0.005 mM, 56% inhibition, 0.025 mM, 90% inhibition; potent inhibition
trilostane
-
treatment does not affect transcription or translation in liver or adrenal
trilostane
specific enzyme inhibitor
trilostane
-
specific inhibitor
additional information

-
structure-inhibition relationship
-
additional information
-
no inhibition by quercetin, apigenin, genistein, daidzein, enterodiol, and enterolactone
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.24
(S)-1-indanol
-
pH 7.4, 25°C
0.093
(S)-1-tetralol
-
pH 7.4, 25°C
0.0084
(S)-phenyl-1-butanol
-
pH 7.4, 25°C
0.089
1,4-Naphthoquinone
-
pH 7.4, 25°C
0.0052
1-phenylisatin
-
pH 7.4, 25°C
0.0038
11beta,17alpha,21-trihydroxy-4-pregnene-3,20-dione
-
-
0.0827
11beta,17alpha,21-trihydroxy-5alpha-pregnane-3,20-dione
-
-
0.0461
11beta,17alpha,21-trihydroxy-5beta-pregnane-3,20-dione
-
-
0.0621
13,14-dihydro-15-keto-prostaglandin F2
-
-
0.13
13,14-dihydro-15-keto-prostaglandin F2alpha
-
-
0.004 - 0.0196
16alpha-hydroxy-dehydroepiandrosterone
0.0014 - 0.0184
16beta-hydroxy-dehydroepiandrosterone
0.206
17-hydroxyprogesterone
-
-
0.0035 - 0.018
17alpha-hydroxypregnenolone
0.684
17beta-hydroxy-5alpha-androstane-3-one
-
-
0.231
17beta-hydroxy-5beta-androstane-3-one
-
-
0.2
2,3-heptanedione
-
pH 7.4, 25°C
0.55
2,3-Hexanedione
-
pH 7.4, 25°C
10
2,3-Pentanedione
-
pH 7.4, 25°C
0.0053
3beta,17alpha-dihydroxy-5-pregen-20-one
-
-
0.0015
4,4'-dimethylbenzil
-
pH 7.4, 25°C
1
4-benzoylpyridine
-
pH 7.4, 25°C
2.097
4-benzylpyridine
-
-
0.319
4-carboxybenzaldehyde
-
-
0.16
4-hexanoylpyridine
-
pH 7.4, 25°C
0.137
4-Nitroacetophenone
-
-
1.966 - 13
4-nitrobenzaldehyde
0.642
5-androsten-3beta-ol-17-one
pH 7.8, 30°C
0.0144 - 0.0147
5-androstene-3,17-dione
0.0167 - 0.0179
5alpha-Androstan-3beta-ol-17-one
0.028 - 1.043
5alpha-androstane-3,17-dione
0.016
5alpha-dihydrotestosterone
-
pH 7.4, 25°C
0.196 - 0.217
5alpha-pregnane-3,20-dione
0.116 - 0.12
5alpha-pregnane-3beta-ol-20-one
0.0049
5beta-androstan-17beta-ol-3-one
-
pH 7.4, 25°C
0.0079
5beta-androstan-3beta-ol-17-one
-
pH 7.4, 25°C
0.01 - 0.123
5beta-androstane-3,17-dione
0.0041
5beta-androstane-3beta,17beta-diol
-
pH 7.4, 25°C
0.0097
5beta-pregnan-20alpha-ol-3-one
-
pH 7.4, 25°C
0.008
5beta-Pregnan-3beta-ol-20-one
-
pH 7.4, 25°C
0.0029 - 1.725
5beta-pregnane-3,20-dione
0.008
5beta-pregnane-3beta,20alpha-diol
-
pH 7.4, 25°C
0.11 - 0.314
5beta-pregnane-3beta-ol-20-one
0.12
6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione
-
pH 7.4, 25°C
0.003
7alpha,27-dihydroxycholesterol
-
-
0.0032
7alpha-hydroxycholesterol
-
-
0.0036 - 2.408
9,10-phenanthrenequinone
0.024
all-trans-retinal
-
pH 7.4, 25°C
0.001
allopregnanolone
-
-
0.13
androst-5-ene-3beta-ol-17-one
-
NAD+ as cofactor
0.00016 - 0.00046
androstanediol
0.0703
androstenedione
-
-
0.005
benzil
-
pH 7.4, 25°C
0.465
D-glucuronic acid
-
-
1.07
D-glyceraldehyde
-
-
0.00009 - 0.221
dehydroepiandrosterone
0.035
Dehydrolithocholic acid
-
pH 7.4, 25°C
54
diacetyl
-
pH 7.4, 25°C
1.4
dimethyl-2-oxo-glutarate
-
pH 7.4, 25°C
0.036
hexanophenone
-
pH 7.4, 25°C
0.32
isatin
-
pH 7.4, 25°C
0.048
n-butyrophenone
-
pH 7.4, 25°C
0.0003
NADPH
-
pH 7.4, 25°C
0.00004 - 0.09
pregnenolone
0.373
prostaglandin E1
-
-
0.0152
prostaglandin E2
-
-
21
Pyridine-4-aldehyde
-
pH 7.4, 25°C
0.0055 - 0.0068
stanolone
0.054
valerophenone
-
pH 7.4, 25°C
additional information
additional information
-
0.004
16alpha-hydroxy-dehydroepiandrosterone

mutant R195R, pH 7.4, 27°C
0.0064
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0148
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0196
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.0014
16beta-hydroxy-dehydroepiandrosterone

wild-type 3beta-HSD1, pH 7.4, 27°C
0.0015
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
0.015
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.0184
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0035
17alpha-hydroxypregnenolone

27°C, pH 7.4
0.018
17alpha-hydroxypregnenolone
27°C, pH 7.4
1.966
4-nitrobenzaldehyde

-
-
13
4-nitrobenzaldehyde
-
pH 7.4, 25°C
0.0144
5-androstene-3,17-dione

-
placental microsomes
0.0147
5-androstene-3,17-dione
-
Sf9 cell homogenate
0.0167
5alpha-Androstan-3beta-ol-17-one

-
placental microsomes
0.0179
5alpha-Androstan-3beta-ol-17-one
-
Sf9 cell homogenate
0.028
5alpha-androstane-3,17-dione

-
pH 7.4, 25°C
1.043
5alpha-androstane-3,17-dione
-
-
0.196
5alpha-pregnane-3,20-dione

pH 7.8, 30°C
0.217
5alpha-pregnane-3,20-dione
-
-
0.116
5alpha-pregnane-3beta-ol-20-one

pH 7.8, 30°C
0.12
5alpha-pregnane-3beta-ol-20-one
-
NAD+ as cofactor
0.01
5beta-androstane-3,17-dione

-
pH 7.4, 25°C
0.123
5beta-androstane-3,17-dione
-
-
0.0029
5beta-pregnane-3,20-dione

-
pH 7.4, 25°C
0.246
5beta-pregnane-3,20-dione
-
-
1.725
5beta-pregnane-3,20-dione
pH 7.8, 30°C
0.11
5beta-pregnane-3beta-ol-20-one

-
NAD+ as cofactor
0.314
5beta-pregnane-3beta-ol-20-one
pH 7.8, 30°C
0.0036
9,10-phenanthrenequinone

-
pH 7.4, 25°C
2.408
9,10-phenanthrenequinone
-
-
0.00016
androstanediol

-
3betaHSD I
0.00046
androstanediol
-
3betaHSD III
0.00009
dehydroepiandrosterone

-
-
0.00012
dehydroepiandrosterone
-
3betaHSD VI
0.00014
dehydroepiandrosterone
-
3betaHSD I
0.00017
dehydroepiandrosterone
-
-
0.00042
dehydroepiandrosterone
-
pH 7.5, 37°C
0.00049
dehydroepiandrosterone
-
3betaHSD III
0.00059
dehydroepiandrosterone
-
37°C, pH 8.4
0.0007
dehydroepiandrosterone
-
wild-type, presence of 2-mercatoethanol pH 7.4
0.001
dehydroepiandrosterone
-
-
0.0016
dehydroepiandrosterone
-
microsomes
0.00164
dehydroepiandrosterone
mutant Q105M, pH 7.4, 27°C
0.0017
dehydroepiandrosterone
-
-
0.0018
dehydroepiandrosterone
-
37°C, pH 7.4
0.0024
dehydroepiandrosterone
-
type I
0.0028
dehydroepiandrosterone
-
mutant C72S, pH 7.4
0.0029
dehydroepiandrosterone
-
type II
0.0037
dehydroepiandrosterone
-
pH 7.4
0.0037
dehydroepiandrosterone
-
wild type enzyme
0.0037
dehydroepiandrosterone
27°C, pH 7.4
0.0037
dehydroepiandrosterone
-
27°C, pH 7.4
0.0037
dehydroepiandrosterone
-
wild-type, pH 7.4
0.0037
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.0037
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.0037
dehydroepiandrosterone
-
in 0.02 M potassium phosphate buffer, pH 7.4
0.0037
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0037
dehydroepiandrosterone
wild-type, pH 7.4, 27°C
0.0044
dehydroepiandrosterone
-
mutant C72S, presence of 2-mercatoethanol pH 7.4
0.0045
dehydroepiandrosterone
-
mitochondria
0.0045
dehydroepiandrosterone
-
modified enzyme
0.005
dehydroepiandrosterone
-
-
0.0061
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
0.0075
dehydroepiandrosterone
-
mutant C83A, presence of 2-mercatoethanol pH 7.4
0.0077
dehydroepiandrosterone
mutant P195R, pH 7.4, 27°C
0.0077
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
0.0082
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
0.01
dehydroepiandrosterone
-
-
0.0107
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.0107
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.011
dehydroepiandrosterone
-
27°C, pH 7.4, S123A mutant 3betaHSD1
0.0115
dehydroepiandrosterone
-
mutant C72F, presence of 2-mercatoethanol pH 7.4
0.0127
dehydroepiandrosterone
-
mutant C72F, pH 7.4
0.0156
dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0156
dehydroepiandrosterone
wild-type, pH 7.4, 27°C
0.0168
dehydroepiandrosterone
-
mutant C111S, presence of 2-mercatoethanol pH 7.4
0.0171
dehydroepiandrosterone
-
mutant C83A, pH 7.4
0.0181
dehydroepiandrosterone
-
mutant C111S, pH 7.4
0.0237
dehydroepiandrosterone
-
mutant C111A, presence of 2-mercatoethanol pH 7.4
0.025
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
0.0268
dehydroepiandrosterone
-
mutant C111A, pH 7.4
0.0327
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
0.0351
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
0.04
dehydroepiandrosterone
mutant Q105M, pH 7.4, 27°C
0.0405
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
0.042
dehydroepiandrosterone
-
27°C, pH 7.4, H156Y mutant
0.042
dehydroepiandrosterone
27°C, pH 7.4, H156Y mutant 3beta-HSD1
0.042
dehydroepiandrosterone
-
pH 7.4, H156Y mutant
0.0424
dehydroepiandrosterone
pH 7.4, 27°C, recombinant mutant H156Y
0.0424
dehydroepiandrosterone
mutant H156Y, pH 7.4, 27°C
0.047
dehydroepiandrosterone
-
27°C, pH 7.4
0.047
dehydroepiandrosterone
27°C, pH 7.4
0.047
dehydroepiandrosterone
-
27°C, pH 7.4
0.0473
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.0473
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.0643
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
0.114
dehydroepiandrosterone
-
-
0.221
dehydroepiandrosterone
-
pH 8.5, 37°C
0.18
menadione

-
pH 7.4, 25°C
0.0000047
NAD+

-
37°C, pH 7.25
0.0034
NAD+
-
wild type enzyme
0.0035
NAD+
-
modified enzyme
0.012
NAD+
-
wild-type, presence of 2-mercatoethanol pH 7.4
0.0153
NAD+
-
mutant C72S, pH 7.4
0.0166
NAD+
-
mutant C111S, presence of 2-mercatoethanol pH 7.4
0.019
NAD+
-
mutant C111S, pH 7.4
0.0194
NAD+
-
mutant C72S, presence of 2-mercatoethanol pH 7.4
0.0205
NAD+
mutant R195P, pH 7.4, 27°C
0.0227
NAD+
-
mutant C111A, presence of 2-mercatoethanol pH 7.4
0.025
NAD+
-
27°C, pH 7.4, H156Y mutant
0.025
NAD+
27°C, pH 7.4, H156Y mutant 3beta-HSD1
0.0255
NAD+
-
mutant C111A, pH 7.4
0.0256
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
0.0299
NAD+
-
mutant C83A, presence of 2-mercatoethanol pH 7.4
0.0333
NAD+
mutant E192A, pH 7.4, 27°C
0.034
NAD+
-
27°C, pH 7.4
0.0341
NAD+
-
wild-type, pH 7.4
0.0341
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.0341
NAD+
-
in 0.02 M potassium phosphate buffer, pH 7.4
0.0341
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0351
NAD+
mutant E192A, pH 7.4, 27°C
0.036
NAD+
-
27°C, pH 7.4, S123A mutant 3betaHSD1
0.0372
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
0.0388
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
0.0468
NAD+
-
mutant C72F, pH 7.4
0.0476
NAD+
-
mutant C72F, presence of 2-mercatoethanol pH 7.4
0.0564
NAD+
mutant R195R, pH 7.4, 27°C
0.0614
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
0.0643
NAD+
mutant D61N, pH 7.4, 27°C
0.0732
NAD+
-
mutant C83A, pH 7.4
0.086
NAD+
-
27°C, pH 7.4
0.0863
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.0863
NAD+
wild-type 3beta-HSD2, pH 7.4, 27°C
0.172
NAD+
-
cosubstrate pregnenolone, pH 8.5, 37°C
0.27
NAD+
-
cosubstrate dehydroepiandrosterone, pH 8.5, 37°C
0.0025
NADH

mutant E192A, pH 7.4, 27°C
0.0036
NADH
mutant R195P, pH 7.4, 27°C
0.0046
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0055
NADH
mutant R195R, pH 7.4, 27°C
0.0065
NADH
mutant E192A, pH 7.4, 27°C
0.0072
NADH
mutant D61N, pH 7.4, 27°C
0.0126
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0003
NADP+

-
pH 7.4, 25°C
0.00004
pregnenolone

-
3betaHSD VI
0.00008
pregnenolone
-
3betaHSD I
0.00047
pregnenolone
-
37°C, pH 7.25
0.001
pregnenolone
-
37°C
0.00103
pregnenolone
-
3betaHSD III
0.0011
pregnenolone
-
37°C
0.002
pregnenolone
-
mitochondria
0.0028
pregnenolone
27°C, pH 7.4
0.0032
pregnenolone
-
microsomes
0.0055
pregnenolone
-
type I
0.0088
pregnenolone
-
type II
0.02
pregnenolone
-
NAD+ as cofactor
0.023
pregnenolone
-
pH 8.5, 37°C
0.05
pregnenolone
27°C, pH 7.4
0.09
pregnenolone
pH 7.8, 30°C
0.0055
stanolone

-
3betaHSD I
0.0068
stanolone
-
3betaHSD III
additional information
additional information

-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic analysis
-
additional information
additional information
-
kinetics recombinant wild-type isozyme and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics recombinant wild-type isozyme and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics recombinant wild-type isozyme and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
-
kinetics recombinant wild-type isozymes and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics recombinant wild-type isozymes and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics recombinant wild-type isozymes and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
-
kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.18
(S)-1-indanol
-
pH 7.4, 25°C
0.25
(S)-1-tetralol
-
pH 7.4, 25°C
0.075
(S)-phenyl-1-butanol
-
pH 7.4, 25°C
0.88
1,4-Naphthoquinone
-
pH 7.4, 25°C
41.7
1-phenylisatin
-
pH 7.4, 25°C
0.07 - 0.15
16alpha-hydroxy-dehydroepiandrosterone
0.04 - 0.15
16beta-hydroxy-dehydroepiandrosterone
40.2
2,3-heptanedione
-
pH 7.4, 25°C
16.3
2,3-Hexanedione
-
pH 7.4, 25°C
26.7
2,3-Pentanedione
-
pH 7.4, 25°C
3.7
4,4'-dimethylbenzil
-
pH 7.4, 25°C
2
4-benzoylpyridine
-
pH 7.4, 25°C
33.3
4-hexanoylpyridine
-
pH 7.4, 25°C
16.7
4-nitrobenzaldehyde
-
pH 7.4, 25°C
0.097
5alpha-androstane-3,17-dione
-
pH 7.4, 25°C
0.095
5alpha-dihydrotestosterone
-
pH 7.4, 25°C
0.083
5beta-androstan-17beta-ol-3-one
-
pH 7.4, 25°C
0.017
5beta-androstan-3beta-ol-17-one
-
pH 7.4, 25°C
0.077
5beta-androstane-3,17-dione
-
pH 7.4, 25°C
0.017
5beta-androstane-3beta,17beta-diol
-
pH 7.4, 25°C
0.07
5beta-pregnan-20alpha-ol-3-one
-
pH 7.4, 25°C
0.041
5beta-Pregnan-3beta-ol-20-one
-
pH 7.4, 25°C
0.063
5beta-pregnane-3,20-dione
-
pH 7.4, 25°C
0.014
5beta-pregnane-3beta,20alpha-diol
-
pH 7.4, 25°C
2
6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione
-
pH 7.4, 25°C
11.8
9,10-phenanthrenequinone
-
pH 7.4, 25°C
0.05
all-trans-retinal
-
pH 7.4, 25°C
0.0233
allopregnanolone
-
-
0.0333
androstenediol
-
-
31.7
benzil
-
pH 7.4, 25°C
0.02 - 11.6
dehydroepiandrosterone
0.043
Dehydrolithocholic acid
-
pH 7.4, 25°C
28.3
diacetyl
-
pH 7.4, 25°C
33.3
dimethyl-2-oxo-glutarate
-
pH 7.4, 25°C
2.7
hexanophenone
-
pH 7.4, 25°C
31.7
isatin
-
pH 7.4, 25°C
0.65
menadione
-
pH 7.4, 25°C
0.85
n-butyrophenone
-
pH 7.4, 25°C
4.7
Pyridine-4-aldehyde
-
pH 7.4, 25°C
2
valerophenone
-
pH 7.4, 25°C
0.07
16alpha-hydroxy-dehydroepiandrosterone

mutant R195R, pH 7.4, 27°C
0.09
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
0.15
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.15
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.04
16beta-hydroxy-dehydroepiandrosterone

wild-type 3beta-HSD1, pH 7.4, 27°C
0.05
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
0.14
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.15
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.02
dehydroepiandrosterone

-
wild-type, pH 7.4
0.021
dehydroepiandrosterone
-
wild-type, presence of 2-mercatoethanol pH 7.4
0.023
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
0.03
dehydroepiandrosterone
-
mutant C111S, presence of 2-mercatoethanol pH 7.4
0.03
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
0.032
dehydroepiandrosterone
-
mutant C111S, pH 7.4
0.0333
dehydroepiandrosterone
-
-
0.04
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
0.047
dehydroepiandrosterone
-
mutant C111A, presence of 2-mercatoethanol pH 7.4
0.048
dehydroepiandrosterone
-
mutant C83A, presence of 2-mercatoethanol pH 7.4
0.05
dehydroepiandrosterone
-
mutant C72S, presence of 2-mercatoethanol pH 7.4
0.05
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
0.053
dehydroepiandrosterone
-
mutant C72S, pH 7.4
0.055
dehydroepiandrosterone
-
27°C, pH 7.4
0.055
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.055
dehydroepiandrosterone
-
mutant C83A, pH 7.4
0.055
dehydroepiandrosterone
-
in 0.02 M potassium phosphate buffer, pH 7.4
0.055
dehydroepiandrosterone
wild-type, pH 7.4, 27°C
0.06
dehydroepiandrosterone
27°C, pH 7.4
0.06
dehydroepiandrosterone
-
mutant C111A, pH 7.4
0.06
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
0.062
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.075
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
0.075
dehydroepiandrosterone
mutant Q105M, pH 7.4, 27°C
0.087
dehydroepiandrosterone
-
mutant C72F, pH 7.4
0.087
dehydroepiandrosterone
-
mutant C72F, presence of 2-mercatoethanol pH 7.4
0.09
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
0.093
dehydroepiandrosterone
mutant P195R, pH 7.4, 27°C
0.113
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
0.113
dehydroepiandrosterone
mutant Q105M, pH 7.4, 27°C
0.115
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.118
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.118
dehydroepiandrosterone
mutant H156Y, pH 7.4, 27°C
0.12
dehydroepiandrosterone
27°C, pH 7.4
0.12
dehydroepiandrosterone
27°C, pH 7.4, H156Y mutant 3beta-HSD1
0.12
dehydroepiandrosterone
pH 7.4, 27°C, recombinant mutant H156Y
0.12
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.13
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.14
dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.145
dehydroepiandrosterone
wild-type, pH 7.4, 27°C
0.217
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
11.6
dehydroepiandrosterone
-
pH 8.5, 37°C
0.02
NAD+

-
wild-type, presence of 2-mercatoethanol pH 7.4
0.02
NAD+
mutant E192A, pH 7.4, 27°C
0.022
NAD+
-
wild-type, pH 7.4
0.022
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
0.031
NAD+
-
mutant C111S, presence of 2-mercatoethanol pH 7.4
0.033
NAD+
-
mutant C111S, pH 7.4
0.038
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
0.04
NAD+
-
mutant C111A, presence of 2-mercatoethanol pH 7.4
0.04
NAD+
mutant E192A, pH 7.4, 27°C
0.043
NAD+
-
mutant C72S, presence of 2-mercatoethanol pH 7.4
0.047
NAD+
-
mutant C72S, pH 7.4
0.048
NAD+
-
mutant C83A, presence of 2-mercatoethanol pH 7.4
0.05
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
0.057
NAD+
-
mutant C83A, pH 7.4
0.058
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.058
NAD+
-
in 0.02 M potassium phosphate buffer, pH 7.4
0.06
NAD+
-
27°C, pH 7.4
0.06
NAD+
mutant D61N, pH 7.4, 27°C
0.063
NAD+
-
mutant C111A, pH 7.4
0.08
NAD+
27°C, pH 7.4, H156Y mutant 3beta-HSD1
0.087
NAD+
-
mutant C72F, pH 7.4
0.087
NAD+
-
mutant C72F, presence of 2-mercatoethanol pH 7.4
0.1
NAD+
mutant R195P, pH 7.4, 27°C
0.11
NAD+
wild-type 3beta-HSD2, pH 7.4, 27°C
0.118
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.145
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
0.17
NAD+
mutant R195R, pH 7.4, 27°C
0.187
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
0.31
NADH

mutant E192A, pH 7.4, 27°C
0.69
NADH
mutant E192A, pH 7.4, 27°C
0.75
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
0.76
NADH
mutant D61N, pH 7.4, 27°C
1.03
NADH
mutant R195P, pH 7.4, 27°C
1.49
NADH
mutant R195R, pH 7.4, 27°C
1.65
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
0.04
pregnenolone

-
-
1.48
pregnenolone
-
pH 8.5, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.