EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
5.1.3.2 | 40 |
- |
recombinant Pisum enzyme loses both activities completely at temperatures higher than 40°C |
702141 |
5.1.3.2 | 41 |
- |
2 min, desensitization by heat converts the enzyme to its ultimate catalytic form |
2321 |
5.1.3.2 | 42 |
- |
7 min, inactivation by dissociation of NAD+ |
2319 |
5.1.3.2 | 45 |
- |
3 min, inactivation by dissociation of NAD+ |
2319 |
5.1.3.2 | 45 |
- |
half-life is 13.5 h |
726782 |
5.1.3.2 | 46 |
- |
no inactivation after several h |
2339 |
5.1.3.2 | 47 |
- |
the melting temperature of the recombinant enzyme is at 47°C |
714602 |
5.1.3.2 | 50 |
- |
inactivation above, monomeric and dimeric form |
2342 |
5.1.3.2 | 50 |
- |
wild-type enzyme loses 20% of its activity after 8 min, mutant enzyme S124A/Y149F loses 80% of its activity after 8 min |
650073 |
5.1.3.2 | 60 |
- |
1 h, stable up to |
721850 |