5.1.3.2	20	-	pH 7.0, 4 h stable, native enzyme	2331	
5.1.3.2	24	-	2 h, stable in presence of 1% bovine serum albumin, 0.5 mM dithiothreitol or 1 mM mercaptoethanol	2317	
5.1.3.2	25	-	60 min, 26% loss of activity	2311	
5.1.3.2	25	-	pH 8.5, 24 h, stable	2343	
5.1.3.2	30	-	53% loss of activity within 10 min, partial protection by NAD+, up to a concentration of 7 mM	2311	
5.1.3.2	30	-	53% loss of activity within 20 min, partial protection by NAD+, up to a concentration of 7 mM	2309	
5.1.3.2	30	70	GalESp2, pH 8.0, stable at	747049	
5.1.3.2	37	-	85% loss of activity within 20 min, partial protection by NAD+, up to a concentration of 7 mM	2309	
5.1.3.2	37	-	85% loss of activity within 20 min, partial protection by NAD+, up to a concentration of 7 mM. Partial protection by addition of NAD+ up to a concentration of 1 mM	2311	
5.1.3.2	40	-	purified recombinant enzyme, complete inactivation above	702141	
5.1.3.2	40	-	recombinant Pisum enzyme loses both activities completely at temperatures higher than 40°C	702141	
5.1.3.2	41	-	2 min, desensitization by heat converts the enzyme to its ultimate catalytic form	2321	
5.1.3.2	42	-	7 min, inactivation by dissociation of NAD+	2319	
5.1.3.2	45	-	3 min, inactivation by dissociation of NAD+	2319	
5.1.3.2	45	-	half-life is 13.5 h	726782	
5.1.3.2	46	-	no inactivation after several h	2339	
5.1.3.2	47	-	the melting temperature of the recombinant enzyme is at 47°C	714602	
5.1.3.2	50	-	inactivation above, monomeric and dimeric form	2342	
5.1.3.2	50	-	wild-type enzyme loses 20% of its activity after 8 min, mutant enzyme S124A/Y149F loses 80% of its activity after 8 min	650073	
5.1.3.2	60	-	1 h, stable up to	721850	
5.1.3.2	60	-	half-life is 23 min	726782	
5.1.3.2	70	-	3 h, no significant decrease in activity	746915	
5.1.3.2	70	-	purified recombinant His6-tagged enzyme, no significant decrease in enzyme activity after 3 h	746915	
5.1.3.2	80	-	3 h, the enzyme retains more than 85% of its original activity	746915	
5.1.3.2	80	-	purified recombinant enzyme, 30 min, loss of 78% activity	702074	
5.1.3.2	80	-	purified recombinant His6-tagged enzyme, retains more than 85% of its original activity under standard assay conditions after 3 h	746915	
5.1.3.2	80	-	treatment at 80°C for 30 min reduces the enzyme activity by 78%	702074	
5.1.3.2	90	-	40 min, almost 50% of its original activity	746915	
5.1.3.2	90	-	enzyme is stable at 90°C, after incubating at this temperature for 10 min, 90% of the enzymatic activity remains	702074	
5.1.3.2	90	-	purified recombinant enzyme, 10 min, loss of 10% activity	702074	
5.1.3.2	90	-	purified recombinant His6-tagged enzyme, retains 50% of its original activity under standard assay conditions after 40 min	746915	
5.1.3.2	100	-	5 min, purified recombinant enzyme, inactivation	704314