EC Number   |
|---|
    6.3.5.4 | - |
| 6.3.5.4 | a fusion protein consisting of the 42 kDa N-terminal region of AS and a 17 kDa tagged-region from pET32a(+) expression plasmid, expression in Escherichia coli |
| 6.3.5.4 | and overexpression |
| 6.3.5.4 | C-terminally tagged enzyme, baculovirus-based expression system, the recombinant enzyme is correctly processed, exhibits high activity and is stable on prolonged storage at -80°C |
| 6.3.5.4 | cDNA, from cell culture SB-P, sequencing |
| 6.3.5.4 | cloned into a 2 mü plasmid, pBS24.1GAS, suitable for replication in a Saccharomyces cerevisiae ciro strain AB116 |
| 6.3.5.4 | cloned into a temperature-sensitive low copy plasmid, pOU71 |
| 6.3.5.4 | cloning and specificity of the amino acid-dependent contol of its mRNA content |
| 6.3.5.4 | cloning of cDNA |
| 6.3.5.4 | DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), enzyme expression analysis |
