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ATP + cysteine sulfinic acid
AMP + diphosphate + cysteine sulfinic acid
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
ATP + L-Asp + L-Gln
AMP + diphosphate + L-Asn + L-Glu
ATP + L-Asp + L-Gln + H2O
AMP + diphosphate + L-Asn + L-Glu
-
-
-
-
?
ATP + L-Asp + NH2OH
AMP + diphosphate + beta-aspartylhydroxamate
ATP + L-Asp + NH3
AMP + diphosphate + Asn
ATP + L-Asp + NH3
AMP + diphosphate + L-Asn
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
CTP + L-Asp + L-Gln
CMP + diphosphate + Asn + Glu
-
weak activity
-
-
?
dATP + L-Asp + L-Gln
dAMP + diphosphate + Asn + Glu
-
utilized at a similar rate as ATP
-
-
?
dATP + L-Asp + NH3
dAMP + diphosphate + Asn
-
utilized at a similar rate as ATP
-
-
?
GTP + L-Asp + L-Gln
GMP + diphosphate + Asn + Glu
L-Glutamic acid gamma-monohydroxamate + H2O
Hydroxylamine + Glu
-
-
-
-
?
L-glutamine
L-glutamate + NH3
L-glutamine + H2O
L-glutamate + NH3
UTP + L-Asp + L-Gln
UMP + diphosphate + Asn + Glu
-
weak activity
-
-
?
additional information
?
-
ATP + L-Asp + L-Gln

AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
the basic region leucine zipper protein ATF5, a transcriptional activator, stimulates asparagine promoter/reporter gene transcription via the nutrient-sensing response unit
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
resistance to L-asparaginase and relapse risk are associated with high expression of asparagine synthetase in TEL-AML1-negative but not in TEL-AML1-positive B-lineage acute lymphoblastic leukemia
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
the ratio of Gln- to NH4+-dependent activity is 2.5
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
ir
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
TaASN1 is dramatically induced by salinity, osmotic stress and exogenous abscisic acid
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
TaASN2 transcripts are very low in all detected tissues and conditions and are only slightly induced by abscisic acid in roots
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
-
-
-
?
ATP + L-Asp + L-Gln

AMP + diphosphate + L-Asn + L-Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + L-Asn + L-Glu
-
-
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + L-Asn + L-Glu
-
light, carbon and nitrogen availability control asparagine synthesis in sunflower by regulating three aspargine synthetase coding genes. HAS2 expression requires light and is positively affected by sucrose. HAS1 and HAS1.1 expression is dependent on nitrogen availability, while HAS2 transcripts are still found in N-starved plants. High ammonium level induces all three asparagine synthetase genes and partially reverts sucrose repression of HAS1 and HAS1.1
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + L-Asn + L-Glu
-
-
-
?
ATP + L-Asp + NH2OH

AMP + diphosphate + beta-aspartylhydroxamate
-
-
-
-
?
ATP + L-Asp + NH2OH
AMP + diphosphate + beta-aspartylhydroxamate
-
-
-
-
?
ATP + L-Asp + NH2OH
AMP + diphosphate + beta-aspartylhydroxamate
-
-
-
-
?
ATP + L-Asp + NH2OH
AMP + diphosphate + beta-aspartylhydroxamate
-
-
-
?
ATP + L-Asp + NH2OH
AMP + diphosphate + beta-aspartylhydroxamate
-
-
-
?
ATP + L-Asp + NH2OH
AMP + diphosphate + beta-aspartylhydroxamate
-
-
-
?
ATP + L-Asp + NH3

AMP + diphosphate + Asn
-
NH4+
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
30% of the activity relative to Gln
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
NH4+
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
the ratio of Gln-dependent to NH4+-dependent activity is 2.5
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
85% of the activity relative to Gln
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH3

AMP + diphosphate + L-Asn
-
-
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + L-Asn
-
-
-
-
?
ATP + L-Asp + NH3
AMP + diphosphate + L-Asn
-
-
-
?
ATP + L-aspartate + L-glutamine

AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
ATP-dependent, mechanism including an enzyme-ATP-Asp-Gln quarternary complex, AS-B structure, two active sites
-
ir
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
glutamine is the in vivo nitrogen source
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
ATP-dependent, the amine group of Gln is transferred directly to Asp
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
transfers the amide group of Gln to Asp
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
ATP-dependent, the amine group of Gln is transferred directly to Asp, maximum activity with 1 mM Gln and 3-10 mM ATP in the assay
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
the enzyme might play a functional role in nitrogen translocation from root to aerial organs in Phaseolus vulgaris
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
ATP-dependent, the amine group of Gln is transferred directly to Asp
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
the reaction sequence begins with the ordered addition of ATP and aspartate. Diphosphate is released, followed by the addition of ammonia and the release of asparagine and AMP. Glutamine is simultaneously hydrolyzed at a second site and the ammonia intermediate diffuses through an interdomain protein tunnel from the site of production to the site of utilization. The data are also consistent with the dead-end binding of asparagine to the glutamine binding site and diphosphate with free enzyme. The rate of hydrolysis of glutamine is largely independent of the activation of aspartate and thus the reaction rates at the two active sites are essentially uncoupled from one another
-
-
?
ATP + L-aspartate + L-glutamine + H2O

AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
ATP in form of MgATP2-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + NH3

AMP + diphosphate + L-asparagine
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
also reaction of EC 6.3.1.1
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
also reaction of EC 6.3.1.1
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
GTP + L-Asp + L-Gln

GMP + diphosphate + Asn + Glu
-
15% of the activity relative to ATP
-
-
?
GTP + L-Asp + L-Gln
GMP + diphosphate + Asn + Glu
-
5.6% of the activity relative to ATP
-
-
?
L-glutamine

L-glutamate + NH3
-
-
-
?
L-glutamine
L-glutamate + NH3
-
-
-
?
L-glutamine
L-glutamate + NH3
-
-
-
?
L-glutamine
L-glutamate + NH3
-
-
-
?
L-glutamine
L-glutamate + NH3
-
-
?
L-glutamine + H2O

L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
?
additional information

?
-
-
physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis
-
?
additional information
?
-
-
physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis
-
?
additional information
?
-
-
comprehensive mechanism has been proposed through which either Gln or NH3 can provide nitrogen for Asn production from Asp
-
-
?
additional information
?
-
-
the N74D As-B mutant exhibits very low glutaminase activity
-
-
?
additional information
?
-
-
no activity with ITP or XTP
-
-
?
additional information
?
-
-
enzyme is extremly selective, being able to discriminate between metabolites that have similar structures to Asp
-
-
?
additional information
?
-
-
enzyme has inherent glutaminase activity
-
-
?
additional information
?
-
-
major biosynthetic pathway for asparagine, AS gene expression is down-regulated by light
-
?
additional information
?
-
-
primary enzyme responsible for asparagine synthesis
-
?
additional information
?
-
-
Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
-
-
?
additional information
?
-
Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
-
-
?
additional information
?
-
Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
-
-
?
additional information
?
-
Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
-
-
?
additional information
?
-
-
enzyme has inherent glutaminase activity
-
-
?
additional information
?
-
-
upregulation of asparagine synthetase fails to avert cell cycle arrest induced by L-asparaginase in TEL/AML1-positive leukaemic cells
-
-
?
additional information
?
-
under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
-
?
additional information
?
-
under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
-
?
additional information
?
-
-
under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
-
?
additional information
?
-
under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
-
?
additional information
?
-
under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
-
?
additional information
?
-
-
under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
-
?
additional information
?
-
-
Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting
-
-
?
additional information
?
-
asparagine is formed in two steps: the beta-carboxylate group of aspartate is first activated by ATP to form an aminoacyl-AMP before its amidation by a nucleophilic attack with an ammonium ion. LdASNA is active and preferentially utilizes ammonia, although it is also capable of utilizing glutamine as a nitrogen source
-
-
?
additional information
?
-
-
asparagine is formed in two steps: the beta-carboxylate group of aspartate is first activated by ATP to form an aminoacyl-AMP before its amidation by a nucleophilic attack with an ammonium ion. LdASNA is active and preferentially utilizes ammonia, although it is also capable of utilizing glutamine as a nitrogen source
-
-
?
additional information
?
-
asparagine is formed in two steps: the beta-carboxylate group of aspartate is first activated by ATP to form an aminoacyl-AMP before its amidation by a nucleophilic attack with an ammonium ion. LdASNA is active and preferentially utilizes ammonia, although it is also capable of utilizing glutamine as a nitrogen source
-
-
?
additional information
?
-
-
the primary site of Asn synthesis is the root and subsequently the leaves receive Asn as the principal N-source for amino acid and protein synthesis
-
-
?
additional information
?
-
-
enzyme is involved in ammonia assimilation
-
-
?
additional information
?
-
-
primary enzyme responsible for asparagine synthesis
-
?
additional information
?
-
-
ATP-diphosphate exchange reaction
-
-
?
additional information
?
-
-
enzyme has inherent glutaminase activity
-
-
?
additional information
?
-
-
Asn, the end product of the mass action of symbiotic NH4+ synthesis, is the principal N-transport-compound of many temperate legumes
-
-
?
additional information
?
-
-
enzyme has inherent glutaminase activity
-
-
?
additional information
?
-
-
enzyme has inherent glutaminase activity
-
-
?
additional information
?
-
-
glutamine or glutamine-derived metabolites regulate AS expression in roots
-
?
additional information
?
-
-
nitrogen metabolism, asparagine synthesis
-
?
additional information
?
-
-
role for hexokinase in the sugar-sensing mechanism that regulates PvNAS2 expression in roots, downregulation of the asparagine synthetase enzyme and concomitantly asparagine production. Thereby a favourable environment is created for the efficient transfer of the amido group of glutamine for the synthesis of purines, and then ureide generation.
-
-
?
additional information
?
-
role for hexokinase in the sugar-sensing mechanism that regulates PvNAS2 expression in roots, downregulation of the asparagine synthetase enzyme and concomitantly asparagine production. Thereby a favourable environment is created for the efficient transfer of the amido group of glutamine for the synthesis of purines, and then ureide generation.
-
-
?
additional information
?
-
-
primary enzyme responsible for asparagine synthesis
-
?
additional information
?
-
-
ATP-diphosphate exchange reaction
-
-
?
additional information
?
-
-
the synthesis of Asn in mammalian tissues proceeds through the intermediate beta-aspartyladenylate
-
-
?
additional information
?
-
-
importance of asparagine synthetase in cell proliferation
-
-
?
additional information
?
-
-
the enzyme continues to produce glutamate even when the synthesis of asparagine has ceased due to a lack of aspartate
-
-
?
additional information
?
-
the enzyme continues to produce glutamate even when the synthesis of asparagine has ceased due to a lack of aspartate
-
-
?
additional information
?
-
the enzyme continues to produce glutamate even when the synthesis of asparagine has ceased due to a lack of aspartate
-
-
?
additional information
?
-
-
possible involvement of the enzyme in the control of metabolic fluxes of carbon and nitrogen through assimilatory pathways
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
ATP + L-Asp + L-Gln
AMP + diphosphate + L-Asn + L-Glu
-
light, carbon and nitrogen availability control asparagine synthesis in sunflower by regulating three aspargine synthetase coding genes. HAS2 expression requires light and is positively affected by sucrose. HAS1 and HAS1.1 expression is dependent on nitrogen availability, while HAS2 transcripts are still found in N-starved plants. High ammonium level induces all three asparagine synthetase genes and partially reverts sucrose repression of HAS1 and HAS1.1
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
L-glutamine
L-glutamate + NH3
L-glutamine + H2O
L-glutamate + NH3
additional information
?
-
ATP + L-Asp + L-Gln

AMP + diphosphate + Asn + Glu
-
the basic region leucine zipper protein ATF5, a transcriptional activator, stimulates asparagine promoter/reporter gene transcription via the nutrient-sensing response unit
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
TaASN1 is dramatically induced by salinity, osmotic stress and exogenous abscisic acid
-
-
?
ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
-
TaASN2 transcripts are very low in all detected tissues and conditions and are only slightly induced by abscisic acid in roots
-
-
?
ATP + L-aspartate + L-glutamine

AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
glutamine is the in vivo nitrogen source
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
-
the enzyme might play a functional role in nitrogen translocation from root to aerial organs in Phaseolus vulgaris
-
-
?
ATP + L-aspartate + L-glutamine + H2O

AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
-
-
-
?
ATP + L-aspartate + NH3

AMP + diphosphate + L-asparagine
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
also reaction of EC 6.3.1.1
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
also reaction of EC 6.3.1.1
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
L-glutamine

L-glutamate + NH3
-
-
-
?
L-glutamine
L-glutamate + NH3
-
-
-
?
L-glutamine
L-glutamate + NH3
-
-
-
?
L-glutamine
L-glutamate + NH3
-
-
-
?
L-glutamine
L-glutamate + NH3
-
-
?
L-glutamine + H2O

L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
?
additional information

?
-
-
physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis
-
?
additional information
?
-
-
physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis
-
?
additional information
?
-
-
comprehensive mechanism has been proposed through which either Gln or NH3 can provide nitrogen for Asn production from Asp
-
-
?
additional information
?
-
-
major biosynthetic pathway for asparagine, AS gene expression is down-regulated by light
-
?
additional information
?
-
-
primary enzyme responsible for asparagine synthesis
-
?
additional information
?
-
-
Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
-
-
?
additional information
?
-
Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
-
-
?
additional information
?
-
Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
-
-
?
additional information
?
-
Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
-
-
?
additional information
?
-
-
upregulation of asparagine synthetase fails to avert cell cycle arrest induced by L-asparaginase in TEL/AML1-positive leukaemic cells
-
-
?
additional information
?
-
under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
-
?
additional information
?
-
under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
-
?
additional information
?
-
-
under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
-
?
additional information
?
-
under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
-
?
additional information
?
-
under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
-
?
additional information
?
-
-
under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
-
?
additional information
?
-
-
Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting
-
-
?
additional information
?
-
-
the primary site of Asn synthesis is the root and subsequently the leaves receive Asn as the principal N-source for amino acid and protein synthesis
-
-
?
additional information
?
-
-
enzyme is involved in ammonia assimilation
-
-
?
additional information
?
-
-
primary enzyme responsible for asparagine synthesis
-
?
additional information
?
-
-
Asn, the end product of the mass action of symbiotic NH4+ synthesis, is the principal N-transport-compound of many temperate legumes
-
-
?
additional information
?
-
-
glutamine or glutamine-derived metabolites regulate AS expression in roots
-
?
additional information
?
-
-
nitrogen metabolism, asparagine synthesis
-
?
additional information
?
-
-
role for hexokinase in the sugar-sensing mechanism that regulates PvNAS2 expression in roots, downregulation of the asparagine synthetase enzyme and concomitantly asparagine production. Thereby a favourable environment is created for the efficient transfer of the amido group of glutamine for the synthesis of purines, and then ureide generation.
-
-
?
additional information
?
-
role for hexokinase in the sugar-sensing mechanism that regulates PvNAS2 expression in roots, downregulation of the asparagine synthetase enzyme and concomitantly asparagine production. Thereby a favourable environment is created for the efficient transfer of the amido group of glutamine for the synthesis of purines, and then ureide generation.
-
-
?
additional information
?
-
-
primary enzyme responsible for asparagine synthesis
-
?
additional information
?
-
-
importance of asparagine synthetase in cell proliferation
-
-
?
additional information
?
-
-
possible involvement of the enzyme in the control of metabolic fluxes of carbon and nitrogen through assimilatory pathways
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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1-methyl-4-(1-methylethyl)-7-oxabicyclo[2.2.1]heptane
-
trivial name 1,4-cineole, almost complete inhibition above 1 mM
2,3-dicarboxypyridine
-
-
2,4-Dicarboxypyridine
-
-
2,5-Dicarboxypyridine
-
-
2,6-dicarboxypyridine
-
-
2-Amino-2-carboxy-L-ethanesulfonamide
-
-
2-Amino-4-arsonophenol hydrochloride
-
-
3,4-Dicarboxypyridine
-
-
3,5-Dicarboxypyridine
-
-
5'-O-[p-(fluorosulfonyl)benzoyl]adenosine
5-Bromo-4-oxo-L-norvaline
-
-
5-Chloro-4-oxo-L-norvaline
5-Diazo-4-oxo-L-norvaline
-
-
6-diazo-5-oxo-L-norleucine
8-N3ATP
-
loss of NH4+-dependent Asn synthesis, but no effect on the glutaminase activity
Adenosine-5'-methylphosphonate
-
-
adenylated sulfoximine
-
0.005 mM, 65% inhibition, dead-end complex with AS-B
alpha,beta-methylene ATP
-
-
alpha,beta-methylene-ATP
-
-
ammonium maleamate
-
weak
AMP-PNP
-
competitive vs. ATP, noncompetitive vs. aspartate, uncompetitive vs. glutamine
aspartic acid analogs
-
-
ATP
-
strong inhibition above 5 mM
beta,gamma-methylene ATP
-
-
beta-asparaginyladenylate
-
-
cis-2-hydroxy-1,4-cineole
-
0.00003 mM, 50% inhibition
Cl-
-
inhibition of the ammonia-dependent reaction, competitive with respect to ammonia, with negative cooperativity. Stimulation of the Gln-dependent and glutaminase reaction
DL-alpha-Aminotricarballylic acid
-
weak
erythro-beta-Hydroxy-L-Asn
-
-
erythro-beta-hydroxy-L-Asp
-
-
erythro-beta-Methyl-L-Asp
-
-
gamma-Methylene-L-Gln
-
-
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydroisoxazol-5-ylacetic acid
-
i.e. NSC-163501
L-2-amino-4-oxo-5-(5'-adenosyl)phosphonopentanoic acid
-
noncompetitive with respect to Gln and uncompetitive with respect to both ATP and Asp
L-2-Amino-4-oxo-5-chloropentanoic acid
-
-
L-2-Amino-4-oxo-5-hydroxypentanoic acid
-
-
L-2-Amino-4-oxo-5-methylphosphonopentanoic acid
-
-
L-beta-Aspartate ethyl ester
-
-
L-cysteinesulfinic acid
-
-
L-glutamate
competitive inhibition
L-Glutamate-gamma-ethyl ester
-
-
L-glutamate-gamma-methyl ester
-
-
L-glutamic acid gamma-methyl ester
-
-
L-glutamic acid gamma-methylester
-
uncompetitive vs. ATP, competitive vs. glutamine
L-Homoserine beta-adenylate
-
in the presence of 30 mM MgCl2
L-methionine sulfoxide
-
-
L-methionine-S-sulfoximine
-
-
meso-Diaminosuccinamate
-
-
N-Benzyloxycarbonyl-L-Asn
-
weak
N-Benzyloxycarbonyl-L-aspartate
-
weak
N-Carbobenzoxy-DL-Gln
-
-
N-Methyl-DL-aspartic acid
-
-
nucleoside triphosphates
-
except ATP
oxaloacetate
-
20 mM, 40% inhibition
p-chloromercuribenzoate
-
-
pyrrolidine-2,3-dicarboxylic acid
-
weak inhibitor
pyruvate
-
20 mM, 12-15% inhibition
S-methyl-L-cysteine-(RS)-sulfoximine
-
weak
sulfoximine adenylate
-
most potent inhibitor
threo-beta-Hydroxy-L-Asn
-
-
threo-beta-methyl-L-Asp
-
-
trans-2-hydroxy-1,4-cineole
-
0.01 mM, 50% inhibition
2-oxoglutarate

-
in presence of aminoxyacetate, 50% inhibition at 5 mM
2-oxoglutarate
-
20 mM, 12-15% inhibition
5'-O-[p-(fluorosulfonyl)benzoyl]adenosine

-
covalently modifies the enzyme
5'-O-[p-(fluorosulfonyl)benzoyl]adenosine
-
Cys523 is the key residue involved in the formation of the 5'-O-[p-(fluorosulfonyl)benzoyl]adenosine-induced disulfide bond, inactivation can be reversed by addition of dithiothreitol
5-Chloro-4-oxo-L-norvaline

-
-
5-Chloro-4-oxo-L-norvaline
-
-
5-Chloro-4-oxo-L-norvaline
-
-
6-diazo-5-oxo-L-norleucine

-
loss of Gln-dependent reactions, but no effect on ATP binding as measured during amminoa-dependent Asn synthesis
6-diazo-5-oxo-L-norleucine
-
-
6-diazo-5-oxo-L-norleucine
-
-
Albizzine

-
-
aminomalonic acid

-
competitive versus L-Asp
aminomalonic acid
-
competitive versus L-Asp
AMP

-
-
AMP
-
2.5 mM, 50% inhibition
AMP
-
noncompetitive versus ATP; poor inhibitor
Asn

-
-
asparagine

-
noncompetitive vs. ATP and aspartate
asparagine
competitive; competitive
azaserine

-
-
beta-methylaspartate

-
weak inhibitor
beta-methylaspartate
-
noncompetitive vs. ATP, competitive vs. aspartate, noncompetitive vs. glutamine
Ca2+

-
-
diphosphate

-
noncompetitive vs. ATP
diphosphate
-
5 mM, 45% inhibition
diphosphate
-
competitive versus ATP
Gln

-
inhibitor of the NH4+-dependent reaction catalyzed by both the C1A and C1S mutants of AS-B
Gln
-
0.4-2.0 mM, inhibits the ammonia-dependent production of Asn
Glu

-
150 mM, 50% inhibition
Glu
-
poor inhibitor; product inhibition
glutamate

-
-
glutamate
competitive; competitive
L-asparagine

-
product inhibition
L-asparagine
competitive inhibition
S-Carbamoylcysteine

-
-
Zn2+

-
-
additional information

L-asparagine has no significant impact on the ammonia-dependent synthetase activity at 1 mM
-
additional information
-
L-asparagine has no significant impact on the ammonia-dependent synthetase activity at 1 mM
-
additional information
-
mouse pancreas contains a proteolytic inhibitor of L-Asn synthetase
-
additional information
-
methionine sulfoximine completely inhibits the NH4+-induced accumulation of AS protein, but not the glutamine-induced accumulation
-
additional information
-
PvNAS2 is downregulated when carbon availability is reduced in nodules
-
additional information
PvNAS2 is downregulated when carbon availability is reduced in nodules
-
additional information
-
enzyme from fetal liver extracts is significantly inhibited when combined with adult liver or tumor extracts
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.38
aspartic acid
-
pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme
11.5 - 17.1
hydroxylamine
1.3
L-aspartic acid
-
pH 8, reaction with NH3, C-terminally tagged recombinant enzyme
0.09 - 0.26
L-glutamic acid gamma-monohydroxamate
additional information
additional information
-
0.53
Asp

-
NH4+-dependent activity, wild-type
0.68
Asp
-
wild-type enzyme
0.85
Asp
-
Asp, wild-type enzyme, Gln-dependent activity
0.013
ATP

-
mutant E348D, glutamine-dependent activity, pH 8.0, 37°C
0.013
ATP
-
mutant E348D, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.03
ATP
-
mutant E348D, ammonia-dependent activity, pH 8.0, 37°C
0.03
ATP
-
mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
0.08
ATP
-
pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme
0.097
ATP
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn3
0.1
ATP
-
wild-type, glutamine-dependent activity, pH 8.0, 37°C
0.1
ATP
-
wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.1 - 1
ATP
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn1
0.106
ATP
-
pH 7.6, 22°C
0.11
ATP
-
pH 8, reaction with NH3, C-terminally tagged recombinant enzyme
0.11
ATP
-
wild-type, ammonia-dependent activity, pH 8.0, 37°C
0.11
ATP
-
wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
0.125
ATP
pH 7.6, temperature not specified in the publication, recombinant nontagged isozyme soluble ZmAsn2
0.128
ATP
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn2
0.128
ATP
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn4
0.18
ATP
-
glutamine dependent asparagine synthetase activity
0.2
ATP
-
ATP, C386A mutant
0.29
ATP
-
C436A mutant and C514A mutant
1.2
ATP
recombinant enzyme, pH 7.8, 37°C
1.47
ATP
pH 7.6, 37°C, recombinant enzyme
0.16
Gln

-
-
0.66
Gln
-
wild-type enzyme
11.5
hydroxylamine

-
wild-type enzyme
17.1
hydroxylamine
-
mutant N74A
0.13
L-Asp

-
mutant E348D, glutamine-dependent activity, pH 8.0, 37°C
0.13
L-Asp
-
mutant E348D, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.23
L-Asp
-
mutant E348D, ammonia-dependent activity, pH 8.0, 37°C
0.23
L-Asp
-
mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
0.58
L-Asp
-
wild-type, glutamine-dependent activity, pH 8.0, 37°C
0.58
L-Asp
-
wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.85
L-Asp
-
pH 7.6, 22°C
1.2
L-Asp
-
wild-type, ammonia-dependent activity, pH 8.0, 37°C
1.2
L-Asp
-
wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
0.1 - 2
L-aspartate

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn3
0.6
L-aspartate
recombinant enzyme, pH 7.8, 37°C
0.68
L-aspartate
-
glutamine dependent asparagine synthetase activity
0.9 - 1
L-aspartate
pH 7.6, temperature not specified in the publication, recombinant nontagged soluble isozyme ZmAsn2
0.93
L-aspartate
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn4
0.98
L-aspartate
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn1
0.98
L-aspartate
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn2
6.21
L-aspartate
pH 7.6, 37°C, recombinant enzyme
0.04
L-Gln

-
-
0.233
L-Gln
pH 7.6, 22°C
0.254
L-Gln
-
pH 7.6, 22°C
0.423
L-Gln
pH 7.6, 22°C
0.543
L-Gln
pH 7.6, 22°C
0.69
L-Gln
-
wild-type enzyme
1.1
L-Gln
-
mutant E348D, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
1.7
L-Gln
-
wild-type, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
2.7
L-Gln
-
mutant E348D, glutaminase activity, pH 8.0, 37°C, ATP absent
3.5
L-Gln
-
mutant E348A, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
3.9
L-Gln
-
mutant E348Q, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
5
L-Gln
-
wild-type, glutaminase activity, pH 8.0, 37°C, ATP absent
5.8
L-Gln
-
mutant E348A, glutaminase activity, pH 8.0, 37°C, ATP absent
9
L-Gln
-
mutant E348Q, glutaminase activity, pH 8.0, 37°C, ATP absent
0.09
L-glutamic acid gamma-monohydroxamate

-
mutant N74A
0.26
L-glutamic acid gamma-monohydroxamate
-
wild-type enzyme
0.26
L-glutamic acid gamma-monohydroxamate
-
ATP, wild-type enzyme, Gln-dependent activity
0.09
L-glutamine

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn2
0.1 - 1
L-glutamine
pH 7.6, temperature not specified in the publication, recombinant nontagged soluble isozyme ZmAsn2
0.233
L-glutamine
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn4
0.423
L-glutamine
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn3
0.543
L-glutamine
pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn1
0.69
L-glutamine
-
glutamine dependent asparagine synthetase activity
0.69
L-glutamine
-
glutamine-dependent synthetase activity
1.39
L-glutamine
-
glutaminase activity in the presence of ATP
1.67
L-glutamine
-
glutaminase activity
1.71
L-glutamine
pH 7.6, 37°C, recombinant enzyme
1.9
L-glutamine
-
glutaminase activity in the absence of ATP
1.9
L-glutamine
-
pH 8, C-terminally tagged recombinant enzyme
10.3
L-glutamine
recombinant enzyme, pH 7.8, 37°C
0.75
NH3

pH 7.6, 22°C
1.12
NH3
pH 7.6, 37°C, recombinant enzyme
1.7
NH3
-
pH 8, C-terminally tagged recombinant enzyme
5.95
NH3
recombinant enzyme, pH 7.8, 37°C
2.1
NH4+

-
-
15.7
NH4+
-
wild-type enzyme
17
NH4+
-
wild-type enzyme
additional information
additional information

-
-
-
additional information
additional information
-
biphasic kinetic, linear portion near 0.5
-
additional information
additional information
-
Km-values of a number of site-specific mutant enzymes
-
additional information
additional information
-
regulation: coregulation by light of the activities of three crucial enzymes of NH4+ assimilation and transport
-
additional information
additional information
-
Km-values of mutant enzymes R30A, R30K, N74A, N74Q, and N79A
-
additional information
additional information
steady-state kinetics
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
-
kinetic mechanism, kinetic model
-
additional information
additional information
the AsnS isozymes are kinetically distinct with substantial differences in Km (Gln) and Vmax values, overview. None of the enzymes has cooperative enzyme kinetics
-
additional information
additional information
the AsnS isozymes are kinetically distinct with substantial differences in Km (Gln) and Vmax values, overview. None of the enzymes has cooperative enzyme kinetics
-
additional information
additional information
the AsnS isozymes are kinetically distinct with substantial differences in Km (Gln) and Vmax values, overview. None of the enzymes has cooperative enzyme kinetics
-
additional information
additional information
the AsnS isozymes are kinetically distinct with substantial differences in Km (Gln) and Vmax values, overview. None of the enzymes has cooperative enzyme kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.03 - 1.17
hydroxylamine
1.3 - 1.7
L-aspartic acid
0.1 - 0.15
L-glutamic acid gamma-monohydroxamate
additional information
additional information
-
0.52
Asp

-
C523A mutant
1.05
Asp
-
wild-type enzyme
0.43
ATP

-
mutant E348D, ammonia-dependent activity, pH 8.0, 37°C
0.43
ATP
-
mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
0.51
ATP
-
mutant E348D, glutamine-dependent activity, pH 8.0, 37°C
0.51
ATP
-
mutant E348D, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.9
ATP
-
wild-type, glutamine-dependent activity, pH 8.0, 37°C
0.9
ATP
-
wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.96
ATP
-
wild-type, ammonia-dependent activity, pH 8.0, 37°C
0.96
ATP
-
wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
1.1
ATP
-
glutamine dependent asparagine synthetase activity
1.6
ATP
-
pH 8, reaction with NH3, C-terminally tagged recombinant enzyme
1.7
ATP
-
pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme
7.46
ATP
pH 7.6, 37°C, recombinant enzyme
0.4
Gln

-
C523A mutant
0.74
Gln
-
C514A mutant, Asp, C436A mutant
1.7
glutamine

-
pH 8, C-terminally tagged recombinant enzyme
1.03
hydroxylamine

-
wild-type enzyme
1.17
hydroxylamine
-
N74A mutant
0.3
L-Asp

-
mutant E348D, ammonia-dependent activity, pH 8.0, 37°C
0.3
L-Asp
-
mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
0.45
L-Asp
-
mutant E348D, glutamine-dependent activity, pH 8.0, 37°C
0.45
L-Asp
-
mutant E348D, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.67
L-Asp
-
wild-type, glutamine-dependent activity, pH 8.0, 37°C
0.67
L-Asp
-
wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.75
L-Asp
-
wild-type, ammonia-dependent activity, pH 8.0, 37°C
0.75
L-Asp
-
wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
1.05
L-aspartate

-
glutamine dependent asparagine synthetase activity
9.19
L-aspartate
pH 7.6, 37°C, recombinant enzyme
1.3
L-aspartic acid

-
pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme
1.7
L-aspartic acid
-
pH 8, reaction with NH3, C-terminally tagged recombinant enzyme
0.05
L-Gln

-
mutant N74A
1.01
L-Gln
-
wild-type enzyme
4
L-Gln
-
mutant E348Q, glutaminase activity, pH 8.0, 37°C, ATP absent
4.1
L-Gln
-
mutant E348D, glutaminase activity, pH 8.0, 37°C, ATP absent
4.45
L-Gln
-
mutant E348Q, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
5.8
L-Gln
-
mutant E348A, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
6.2
L-Gln
-
wild-type, glutaminase activity, pH 8.0, 37°C, ATP absent
6.37
L-Gln
-
mutant E348A, glutaminase activity, pH 8.0, 37°C, ATP absent
6.6
L-Gln
-
wild-type, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
10.02
L-Gln
-
mutant E348D, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
0.1
L-glutamic acid gamma-monohydroxamate

-
mutant N74A
0.15
L-glutamic acid gamma-monohydroxamate
-
wild-type
0.8
L-glutamine

-
glutaminase activity in the absence of ATP
1.01
L-glutamine
-
glutamine dependent asparagine synthetase activity
1.38
L-glutamine
-
glutaminase activity in the presence of ATP
2.73
L-glutamine
-
glutamine-dependent synthetase activity
3 - 6
L-glutamine
-
glutaminase activity
3 - 6
L-glutamine
-
glutamine-dependent synthetase activity
3.38
L-glutamine
-
glutaminase activity
4.51
L-glutamine
pH 7.6, 37°C, recombinant enzyme
6.08
L-glutamine
-
glutaminase activity in the presence of ATP
6.08
L-glutamine
-
glutamine dependent asparagine synthetase activity
1.8
NH3

-
pH 8, C-terminally tagged recombinant enzyme
4.18
NH3
pH 7.6, 37°C, recombinant enzyme
0.59
NH4+

-
wild-type
additional information
additional information

-
turnover-numbers of mutant enzymes R30A, R30K, N74A, N74Q, and N79A
-
additional information
additional information
-
turnover-numbers of a number of site-specific AS-B mutant enzymes
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.84
L-aspartate
pH 7.6, 37°C, recombinant enzyme
2.64
L-glutamine
pH 7.6, 37°C, recombinant enzyme
6.66
NH3
pH 7.6, 37°C, recombinant enzyme
1.48
ATP

pH 7.6, 37°C, recombinant enzyme
8.7
ATP
-
wild-type, ammonia-dependent activity, pH 8.0, 37°C
8.7
ATP
-
wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
9
ATP
-
wild-type, glutamine-dependent activity, pH 8.0, 37°C
9
ATP
-
wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
14.3
ATP
-
mutant E348D, ammonia-dependent activity, pH 8.0, 37°C
14.3
ATP
-
mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
39.2
ATP
-
mutant E348D, glutamine-dependent activity, pH 8.0, 37°C
39.2
ATP
-
mutant E348D, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.63
L-Asp

-
wild-type, ammonia-dependent activity, pH 8.0, 37°C
0.63
L-Asp
-
wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
1.2
L-Asp
-
wild-type, glutamine-dependent activity, pH 8.0, 37°C
1.2
L-Asp
-
wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
1.3
L-Asp
-
mutant E348D, ammonia-dependent activity, pH 8.0, 37°C
1.3
L-Asp
-
mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C
3.4
L-Asp
-
mutant E348D, glutamine-dependent activity, pH 8.0, 37°C
3.45
L-Asp
-
mutant E348D, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C
0.45
L-Gln

-
mutant E348Q, glutaminase activity, pH 8.0, 37°C, ATP absent
1.1
L-Gln
-
mutant E348A, glutaminase activity, pH 8.0, 37°C, ATP absent
1.14
L-Gln
-
mutant E348Q, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
1.19
L-Gln
-
wild-type, glutaminase activity, pH 8.0, 37°C, ATP absent
1.51
L-Gln
-
mutant E348D, glutaminase activity, pH 8.0, 37°C, ATP absent
1.66
L-Gln
-
mutant E348A, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
3.88
L-Gln
-
wild-type, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
9.1
L-Gln
-
mutant E348D, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.