EC Number   |
|---|
    6.1.1.1 | - |
| 6.1.1.1 | a library of more than 200 mutants substituting the ATP binding motif KMSSS, Lys204-Met205-Ser206-Ser207-Ser208, is built, mutants and wild-type of MjYRS are cloned into the vector pET41a+ |
| 6.1.1.1 | catalytic domain (residues 1-322) |
| 6.1.1.1 | CYT-18 is expressed from the plasmid pEX560 in the Escherichia coli strain HMS174DE3 |
| 6.1.1.1 | DNA and amino acid sequence determination and analysis of two enzymes types, expression of tyrosyl aminoacyl-tRNA synthetase as C-terminally His6-tagged enzyme in Escherichia coli |
| 6.1.1.1 | DNA and amino acid sequence determination and analysis, RT-PCR expression analysis, phylogenetic analysis and tree |
| 6.1.1.1 | DNA and amino acid sequence determination of wild-type and mutant enzymes, localization of the gene encoding the enzyme on chromosome 1p34-p35, functional complementation of a Saccharomyces cerevisiae TYS1 mutant strain, overview, transient expression of EGFP-tagged wild-type and mutant enzymes in murine neuroblastoma N2a cells |
| 6.1.1.1 | expressed in Escherichia coli |
| 6.1.1.1 | expressed in Escherichia coli as a His-tagged fusion protein |
| 6.1.1.1 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells |
