Information on EC 6.1.1.1 - tyrosine-tRNA ligase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.1.1.1
-
RECOMMENDED NAME
GeneOntology No.
tyrosine-tRNA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-tyrosine + tRNATyr = AMP + diphosphate + L-tyrosyl-tRNATyr
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acylation
esterification
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tRNA charging
-
-
tyrosine metabolism
-
-
Aminoacyl-tRNA biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
L-tyrosine:tRNATyr ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9023-45-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. Jingsong x Haoyue
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Methanocaldococcus jannaschii TCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440
i.e. Methanocaldococcus jannaschii
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
-
UniProt
Manually annotated by BRENDA team
Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3
-
Uniprot
Manually annotated by BRENDA team
purified recombinant enzyme
-
-
Manually annotated by BRENDA team
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
purified recombinant enzyme
-
-
Manually annotated by BRENDA team
wheat
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
AMP + diphosphate
ATP
show the reaction diagram
ATP + 3-(2-naphthyl)alanine + tRNATyr
AMP + diphosphate + 3-(2-naphthyl)alanyl-tRNATyr
show the reaction diagram
ATP + 3-(5-hydroxypyridin-2-yl)-L-alanine + tRNATyr
?
show the reaction diagram
-
-
-
-
?
ATP + 3-amino-L-tyrosine + tRNATyr
AMP + 3-amino-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + 3-azido-L-tyrosine + tRNATyr
AMP + 3-azido-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + 3-azido-L-tyrosine + tRNATyr
AMP + diphosphate + 3-azido-L-tyrosyl-tRNATyr
show the reaction diagram
ATP + 3-chloro-L-tyrosine + tRNATyr
AMP + 3-chloro-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + 3-iodo-L-tyrosine + tRNATyr
AMP + 3-iodo-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + 3-iodo-L-tyrosine + tRNATyr
AMP + diphosphate + 3-iodo-L-tyrosyl-tRNATyr
show the reaction diagram
ATP + 3-iodo-L-tyrosine + tRNATyr
AMP + L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y73V/Q195C and other mutants, no activity with the wild-type enzyme
-
?
ATP + 3-methoxy-L-tyrosine + tRNATyr
AMP + 3-methoxy-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + 3-nitro-L-tyrosine + tRNATyr
AMP + 3-nitro-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + 4-acetylphenylalanine + tRNATyr
AMP + diphosphate + 4-acetylphenylalanyl-tRNATyr
show the reaction diagram
ATP + 4-bromophenylalanine + tRNATyr
AMP + diphosphate + 4-bromophenylalanyl-tRNATyr
show the reaction diagram
ATP + D-3,4-dihydroxyphenylalanine + tRNATyr
AMP + D-3,4-dihydroxyphenylalanine-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + D-tyrosine + tRNATyr
AMP + D-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + D-tyrosine + tRNATyr
AMP + diphosphate + D-tyrosyl-tRNATyr
show the reaction diagram
-
enzyme TyrRS has a detectable, natural activity for the D-tyrosine stereoisomer, only tenfold less than for L-Tyr
-
-
?
ATP + L-3,4-dihydroxyphenylalanine + tRNATyr
AMP + L-3,4-dihydroxyphenylalanine-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + L-beta-(5-hydroxy-2-pyridyl)-alanine + tRNATyr
AMP + L-beta-(5-hydroxy-2-pyridyl)-alanine-tRNATyr + diphosphate
show the reaction diagram
ATP + L-tyrosine + tRNATyr
AMP + diphosphate + L-tyrosyl-tRNATyr
show the reaction diagram
ATP + L-tyrosine + tRNATyr
AMP + L-Tyr-tRNATyr + diphosphate
show the reaction diagram
ATP + L-tyrosine + tRNATyr(G34C)
AMP + diphosphate + L-tyrosyl-tRNATyr(G34C)
show the reaction diagram
ATP + L-tyrosine + tRNATyr(wild-type)
AMP + diphosphate + L-tyrosyl-tRNATyr(wild-type)
show the reaction diagram
ATP + L-tyrosine + tRNATyrGTA
AMP + diphosphate + L-tyrosyl-tRNATyrGTA
show the reaction diagram
two sequences, tRNATyr1 and tRNATyr2, substrate CHO tRNATyrGTA is recombinantly expressed. CHOTyrRS is able to attach Phe to both tRNATyr2 and CHO total tRNA containing native tRNATyr, excluding the possibility that mischarging results from the lack of post-transcriptional modifications to the in vitro-transcribed substrate
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-
?
ATP + m-fluoro-D,L-tyrosine + tRNATyr
AMP + m-fluoro-D,L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
mutant Y43G, aminoacylation assay
-
?
ATP + N-acetyl-L-tyrosine + tRNATyr
AMP + N-acetyl-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
wild-type and mutant Y43G, aminoacylation assay
-
?
ATP + N-formyl-L-tyrosine + tRNATyr
AMP + N-formyl-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
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wild-type and mutant Y43G, aminoacylation assay
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?
ATP + N-o-nitrophenylsulfenyl-L-tyrosine + tRNATyr
AMP + N-o-nitrophenylsulfenyl-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
wild-type and mutant Y43G, aminoacylation assay
-
?
ATP + O-dansyl-L-tyrosine + tRNATyr
AMP + O-dansyl-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
wild-type and mutant Y43G, aminoacylation assay
-
?
ATP + O-methyl-L-tyrosine + tRNATyr
AMP + O-methyl-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
ATP + O-phospho-L-tyrosine + tRNATyr
AMP + O-phospho-L-Tyr-tRNATyr + diphosphate
show the reaction diagram
-
wild-type and mutant Y43G, aminoacylation assay
-
?
ATP + p-acetyl-L-phenylalanine + tRNATyr
AMP + diphosphate + p-acetyl-L-phenylalanyl-tRNATyr
show the reaction diagram
-
aminoacyl-tRNA synthetases are designed through a combination of homology modeling, molecular docking and binding affinity computation with the purpose of incorporating pACPhe into proteins in Escherichia coli
-
-
?
ATP + p-iodophenylalanine + tRNATyr
AMP + diphosphate + p-iodophenylalanyl-tRNATyr
show the reaction diagram
-
a variant of the Methanococcus jannaschii tyrosyl synthetase that selectively incorporates para-iodophenylalanine in response to an amber stop codon is identified
-
-
?
ATP + tyrosine + tRNATyr
?
show the reaction diagram
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-
-
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ATP + tyrosine + tRNATyr
AMP + Tyr-tRNATyr + diphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 3-(2-naphthyl)alanine + tRNATyr
AMP + diphosphate + 3-(2-naphthyl)alanyl-tRNATyr
show the reaction diagram
-
activity of a natural mutant enzyme, NpAla TyrRS activity
-
-
?
ATP + 4-acetylphenylalanine + tRNATyr
AMP + diphosphate + 4-acetylphenylalanyl-tRNATyr
show the reaction diagram
-
activity of a natural mutant enzyme
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-
?
ATP + 4-bromophenylalanine + tRNATyr
AMP + diphosphate + 4-bromophenylalanyl-tRNATyr
show the reaction diagram
-
activity of a natural mutant enzyme, p-BrPhe TyrRS activity
-
-
?
ATP + L-beta-(5-hydroxy-2-pyridyl)-alanine + tRNATyr
AMP + L-beta-(5-hydroxy-2-pyridyl)-alanine-tRNATyr + diphosphate
show the reaction diagram
-
L-beta-(5-hydroxy-2-pyridyl)-alanine i.e. azatyrosine, mutant F130S shows 17fold higher activity in vivo than the wild-type enzyme
-
?
ATP + L-tyrosine + tRNATyr
AMP + diphosphate + L-tyrosyl-tRNATyr
show the reaction diagram
ATP + L-tyrosine + tRNATyr
AMP + L-Tyr-tRNATyr + diphosphate
show the reaction diagram
ATP + tyrosine + tRNATyr
?
show the reaction diagram
-
-
-
-
-
ATP + tyrosine + tRNATyr
AMP + Tyr-tRNATyr + diphosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-[4-[([4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl]oxy)carbonyl]phenyl]dibroman-1-ium
-
-
-
3-(3,4-dichlorophenyl)-4-(2-(4-methylpiperazin-1-yl)ethoxy)furan-2(5H)-one
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-
3-(3,4-difluorophenyl)-4-(2-(piperidin-1-yl)ethoxy)furan-2(5H)-one
-
-
3-(3,4-dimethoxyphenyl)-4-(2-(4-methylpiperazin-1-yl)ethoxy)furan-2(5H)-one
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-
3-(3,4-dimethoxyphenyl)-4-(2-(piperidin-1-yl)ethoxy)furan-2(5H)-one
-
-
3-(3-bromophenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
-
-
3-(3-chlorophenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
-
-
3-(3-hydroxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
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3-(3-methoxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
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3-(4-bromophenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
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-
3-(4-chlorophenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
-
-
3-(4-hydroxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
-
replacement of the morpholine-ring in the side chain of the compound with a substituent containing more hydrophilic groups is probably more suitable for further modification. Most potent agent against Staphylococcus aureus ATCC 25923 with MIC50 value of 0.00023 mg/ml
3-(4-methoxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
-
-
3-arylfuran-2(5H)-one
-
-
-
3-phenyl-4-(2-(piperidin-1-yl)ethoxy)furan-2(5H)-one
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-
3-phenyl-4-(2-(propylamino)ethoxy)furan-2(5H)-one
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-
3-phenyl-4-(2-(pyrrolidin-1-yl)ethoxy)furan-2(5H)-one
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-
4-(2-(2,4-dichlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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-
4-(2-(2,4-dihydroxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(2,5-dichlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(2-(4-nitrophenyl)hydrazinyl)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(2-chlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(2-hydroxyphenylformyloxyl)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(2-methoxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(2-methylphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(2-nitrophenylformyloxyl)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(3,4-dichlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(3,4-dihydroxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(3,4-dimethoxyphenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(3,5-dihydroxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(3-bromophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
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4-(2-(3-chlorophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
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4-(2-(3-chlorophenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(3-chlorophenylformyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
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4-(2-(3-methylphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(3-nitrophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(3-pyridylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
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4-(2-(3-trifluoromethylphenylformyoxy)ethoxy)-3-phenylfuran-2(5H)-one
-
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4-(2-(4-bromophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
-
-
4-(2-(4-bromophenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
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4-(2-(4-chlorophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
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4-(2-(4-chlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
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4-(2-(4-fluorophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
-
-
4-(2-(4-fluorophenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-(4-fluorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
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4-(2-(4-hydroxyphenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
-
-
4-(2-(4-hydroxyphenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-(4-hydroxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-(4-methylphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-(4-methylpiperazin-1-yl)ethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-(4-nitrophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-(6-hydroxynaphthalen-2-ylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
-
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4-(2-(benzylamino)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(butylamino)ethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-(cyclohexylamino)ethoxy)-3-(3,4-dimethoxyphenyl)furan-2(5H)-one
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4-(2-(cyclohexylamino)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-(naphthalen-2-ylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
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4-(2-butyryloxyethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-hexanoyloxyethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-morpholinoethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-octanoyloxyethoxy)-3-phenylfuran-2(5H)-one
-
-
4-(2-phenylacetyloxylethoxy)-3-phenylfuran-2(5H)-one
-
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4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-bromobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-chlorobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-chloropyridine-3-carboxylate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-fluorobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-methoxybenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-methylbenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-nitrobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-(3-methylphenyl)propanoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-(4-methylphenyl)propanoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-aminobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-bromobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-chlorobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-fluorobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-hydroxybenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-methylbenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-phenylpropanoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-aminobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-chlorobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-fluorobenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-hydroxybenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-methylbenzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 6-chloropyridine-3-carboxylate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 6-hydroxypyridine-3-carboxylate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl benzoate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl pyridine-3-carboxylate
-
-
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl pyridine-4-carboxylate
-
-
5'-O-[N-(L-tyrosyl)sulfamoyl]adenosine
-
i.e. TyrSA, a tyrosyl adenylate analogue,. TyrSA is engaged in many interactions with active site residues occupying the tyrosine and adenine binding pockets. Residues making hydrogen bonds with the TyrSA tyrosyl group in the tyrosine binding pocket (YBP) are Y36, Y163, Q167, D170 and Q185. Residues G38, A72 and F75 are responsible for the hydrophobic interactions between enzyme and tyrosine moiety in the YBP
-
6-Aminomethyladenosine triphosphate
-
-
acacetin
chloride
chrysin
diphosphate
epigallocatechin gallate
-
strong inhibition
fisetin
kaempferide
-
strong inhibition
L-tyrosinyl 1,4-anhydro-D-ribitol-5-O-phosphate
L-tyrosinyl 1-beta-naphthyl-1,4-anhydro-D-ribitol-5-O-phosphate
L-tyrosinyl N6-benzoyl adenylate
-
-
L-tyrosinyl uridine-5'-O-phosphate
L-tyrosinyl-2',3'-O-isopropylidene adenylate
L-tyrosinyl-2'-deoxy adenylate
L-tyrosinyl-3'-deoxy adenylate
N-(2-chlorophenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(3,5-dichlorophenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(3,5-difluorophenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(3,5-dimethoxyphenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(3-chlorophenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(3-nitrophenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(4-bromophenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(4-bromophenyl)-N2-[(2-chlorophenyl)acetyl]glycinamide
-
-
N-(4-bromophenyl)-N2-[(3-bromophenyl)acetyl]glycinamide
-
-
N-(4-bromophenyl)-N2-[(3-chlorophenyl)acetyl]glycinamide
-
-
N-(4-chlorophenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(4-methoxyphenyl)-N2-(phenylacetyl)glycinamide
-
-
N-(4-nitrophenyl)-N2-(phenylacetyl)glycinamide
-
-
N-ethylmaleimide
-
-
N-phenyl-N2-(phenylacetyl)glycinamide
-
-
N2-[(2-chlorophenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(2-chlorophenyl)acetyl]-N-(4-nitrophenyl)glycinamide
-
-
N2-[(2-hydroxyphenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(3,4-dimethoxyphenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(3,4-dimethoxyphenyl)acetyl]-N-(4-nitrophenyl)glycinamide
-
-
N2-[(3-chlorophenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(3-chlorophenyl)acetyl]-N-(4-methylphenyl)glycinamide
-
-
N2-[(3-chlorophenyl)acetyl]-N-(4-nitrophenyl)glycinamide
-
-
N2-[(3-fluorophenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(3-fluorophenyl)acetyl]-N-(4-nitrophenyl)glycinamide
-
-
N2-[(3-hydroxyphenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(3-hydroxyphenyl)acetyl]-N-(4-nitrophenyl)glycinamide
-
-
N2-[(4-chlorophenyl)acetyl]-N-(3,5-dichlorophenyl)glycinamide
-
-
N2-[(4-chlorophenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(4-fluorophenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(4-fluorophenyl)acetyl]-N-(4-nitrophenyl)glycinamide
-
-
N2-[(4-hydroxyphenyl)acetyl]-N-(4-methoxyphenyl)glycinamide
-
-
N2-[(4-hydroxyphenyl)acetyl]-N-(4-nitrophenyl)glycinamide
-
-
N2-[(4-methylphenyl)acetyl]-N-(4-nitrophenyl)glycinamide
-
-
O-(adenosine-5'-O-yl) N-(L-tyrosyl)phosphoramidate
i.e. Tyr-AMPN, a non-hydrolyzable Tyr-AMP analog, binding structure, overview
resveratrol
inhibits the enzyme activity and the growth of promastigotes
sulfate
tRNATyr
-
-
tyrosinol
-
binding structure, overview
tyrosinyl adenylate
tyrosyl aryl dipeptides
-
inhibitor interacts with and occupies the key catalytic residues in the tyrosyl binding pocket of the catalytic site
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.04 - 2.43
3-chloro-L-tyrosine
0.625 - 1.3
3-fluoro-D,L-tyrosine
0.13 - 1.15
3-iodo-L-tyrosine
1.6
A22G mutated tRNATyr transcript
-
-
-
0.00043 - 7
ATP
0.46 - 14
D-tyrosine
0.8
G15A mutated tRNATyr transcript
-
-
-
22 - 32
K+
0.56 - 1.84
L-3,4-dihydroxyphenylalanine
0.018 - 0.038
L-beta-(5-hydroxy-2-pyridyl)-alanine
0.0003 - 1.19
L-tyrosine
0.002
native yeast tRNATyr
-
pH 7.5, 30°C
-
0.000022 - 4.8
tRNATyr
0.00068 - 0.039
tRNATyr(G34C)
-
0.00035 - 0.0014
tRNATyr(wild-type)
-
0.0015
tRNYTyr
-
-
-
0.002 - 0.013
tyrosine
0.0002
tyrosyl-tRNATyr
-
deacylation
-
2.2
U54C mutated tRNATyr transcript
-
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13 - 6.08
3-chloro-L-tyrosine
0.62 - 6.08
3-fluoro-D,L-tyrosine
0.43 - 0.48
3-iodo-L-tyrosine
0.00003
A22G mutated tRNATyr transcript
-
-
-
0.1 - 14.2
ATP
0.37 - 1.2
D-tyrosine
0.0002
G15A mutated tRNATyr transcript
-
-
-
0.67 - 6.08
L-3,4-dihydroxyphenylalanine
0.042 - 0.11
L-beta-(5-hydroxy-2-pyridyl)-alanine
0.01 - 45
L-tyrosine
1.5
native yeast tRNATyr
-
pH 7.5, 30°C
-
0.046 - 6.08
tRNATyr
0.07 - 0.079
tRNATyr(G34C)
-
0.12 - 0.19
tRNATyr(wild-type)
-
0.012
U54C mutated tRNATyr transcript
-
-
-
additional information
additional information
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
140 - 33020
ATP
10 - 1990
L-tyrosine
0.33 - 1318
tRNATyr
additional information
additional information
-
kinetic parameters for anticodon mutants of tRNATyr and for for acceptor stem mutants of tRNATyr
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.32 - 2.15
AMP
0.048 - 0.23
diphosphate
0.076 - 0.42
L-tyrosinyl 1,4-anhydro-D-ribitol-5-O-phosphate
0.0057 - 0.15
L-tyrosinyl 1-beta-naphthyl-1,4-anhydro-D-ribitol-5-O-phosphate
0.000011
L-tyrosinyl adenylate
-
pH 7.9, 37°C
0.00068
L-tyrosinyl N6-benzoyl adenylate
-
pH 7.9, 37°C
0.00021
L-tyrosinyl uridine-5'-O-phosphate
-
pH 7.9, 37°C
0.0000093 - 0.000026
L-tyrosinyl-2',3'-O-isopropylidene adenylate
0.018 - 0.1
L-tyrosinyl-2'-deoxy adenylate
0.036 - 0.14
L-tyrosinyl-3'-deoxy adenylate
0.0000063
tyrosinyl adenylate
-
pH 7.9, 37°C
additional information
additional information
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05117
1-[4-[([4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl]oxy)carbonyl]phenyl]dibroman-1-ium
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
-
0.1
3-(3,4-dichlorophenyl)-4-(2-(4-methylpiperazin-1-yl)ethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
above, pH not specified in the publication, temperature not specified in the publication
0.1
3-(3,4-difluorophenyl)-4-(2-(piperidin-1-yl)ethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
above, pH not specified in the publication, temperature not specified in the publication
0.1
3-(3,4-dimethoxyphenyl)-4-(2-(4-methylpiperazin-1-yl)ethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
above, pH not specified in the publication, temperature not specified in the publication
0.1
3-(3,4-dimethoxyphenyl)-4-(2-(piperidin-1-yl)ethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
above, pH not specified in the publication, temperature not specified in the publication
0.0243
3-(3-bromophenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0205
3-(3-chlorophenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0256
3-(3-hydroxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0375
3-(3-methoxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0024
3-(4-bromophenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.00062
3-(4-chlorophenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0001
3-(4-hydroxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0328
3-(4-methoxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0536
3-phenyl-4-(2-(piperidin-1-yl)ethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0291
3-phenyl-4-(2-(propylamino)ethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0355
3-phenyl-4-(2-(pyrrolidin-1-yl)ethoxy)furan-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0165
4-(2-(2,4-dichlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0502
4-(2-(2,4-dihydroxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0052
4-(2-(2,5-dichlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.1
4-(2-(2-(4-nitrophenyl)hydrazinyl)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
above, pH not specified in the publication, temperature not specified in the publication
0.0568
4-(2-(2-chlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0414
4-(2-(2-hydroxyphenylformyloxyl)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0647
4-(2-(2-methoxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.1
4-(2-(2-methylphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
IC50 above 0.1 mM, in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0182
4-(2-(2-nitrophenylformyloxyl)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0334
4-(2-(3,4-dichlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0753
4-(2-(3,4-dihydroxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0838
4-(2-(3,4-dimethoxyphenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0067
4-(2-(3,5-dihydroxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0035
4-(2-(3-bromophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0011
4-(2-(3-chlorophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.1
4-(2-(3-chlorophenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
IC50 above 0.1 mM, in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0009
4-(2-(3-chlorophenylformyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.081
4-(2-(3-methylphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.1
4-(2-(3-nitrophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
IC50 above 0.1 mM, in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.026
4-(2-(3-pyridylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0043
4-(2-(3-trifluoromethylphenylformyoxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0167
4-(2-(4-bromophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0481
4-(2-(4-bromophenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0093
4-(2-(4-chlorophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0088
4-(2-(4-chlorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0057
4-(2-(4-fluorophenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0936
4-(2-(4-fluorophenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0075
4-(2-(4-fluorophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0079
4-(2-(4-hydroxyphenylacetyloxy)ethoxy)-3-(4-chlorophenyl)furan-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.1
4-(2-(4-hydroxyphenylacetyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
IC50 above 0.1 mM, in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0112
4-(2-(4-hydroxyphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0178
4-(2-(4-methylphenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0086
4-(2-(4-methylpiperazin-1-yl)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0052
4-(2-(4-nitrophenylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0168
4-(2-(6-hydroxynaphthalen-2-ylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.1
4-(2-(benzylamino)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
above, pH not specified in the publication, temperature not specified in the publication
0.0719
4-(2-(butylamino)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.1
4-(2-(cyclohexylamino)ethoxy)-3-(3,4-dimethoxyphenyl)furan-2(5H)-one
Staphylococcus aureus;
-
above, pH not specified in the publication, temperature not specified in the publication
0.0874
4-(2-(cyclohexylamino)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.0177
4-(2-(naphthalen-2-ylformyloxy)ethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0846
4-(2-butyryloxyethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0478
4-(2-hexanoyloxyethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0062
4-(2-morpholinoethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
pH not specified in the publication, temperature not specified in the publication
0.041
4-(2-octanoyloxyethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.0128
4-(2-phenylacetyloxylethoxy)-3-phenylfuran-2(5H)-one
Staphylococcus aureus;
-
in 100 mM Tris/Cl pH 7.9, 50 mM KCl, 16 mM MgCl2, at 37°C
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-bromobenzoate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-chlorobenzoate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-chloropyridine-3-carboxylate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-fluorobenzoate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
0.0921
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-methoxybenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.08862
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-methylbenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 2-nitrobenzoate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
0.01898
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-(3-methylphenyl)propanoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.0111
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-(4-methylphenyl)propanoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.07109
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-aminobenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.08313
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-bromobenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.08613
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-chlorobenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.08214
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-fluorobenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.07211
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-hydroxybenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.069
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-methylbenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.083
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 3-phenylpropanoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.04894
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-aminobenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.05523
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-chlorobenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.06514
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-fluorobenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.04476
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-hydroxybenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.05008
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 4-methylbenzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 6-chloropyridine-3-carboxylate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl 6-hydroxypyridine-3-carboxylate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
0.04332
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl benzoate
Staphylococcus aureus;
-
pH 7.9, 37°C, recombinant enzyme
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl pyridine-3-carboxylate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
0.1
4-[(naphthalen-1-yl)methyl]-5-oxo-2,5-dihydrothiophen-3-yl pyridine-4-carboxylate
Staphylococcus aureus;
-
above, pH 7.9, 37°C, recombinant enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000076
-
purified recombinant His-tagged enzyme
1.8
-
purified enzyme
18.35
-
-
1
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
-
aminoacylation
1
7.2
-
assay at
7.5 - 9
-
-
7.5 - 9
-
ATP-diphosphate exchange reaction
1
7.7 - 8
-
kinetic assays