EC Number |
---|
5.3.4.1 | - |
5.3.4.1 | baculovirus expression system |
5.3.4.1 | cDNA cloning from library, DNA and amino acid sequence determination and analysis, genetic structure comparison and phylogenetic tree |
5.3.4.1 | cloning from RNA by 3' race RT-PCR, DNA and amino acid sequence determination and analysis, sequence comparison, expression as GST-tagged or His-tagged enzyme in Escherichia coli strain BL21(DE3) |
5.3.4.1 | cloning of cDNA |
5.3.4.1 | cloning of the a and a' domain, expression in Escherichia coli |
5.3.4.1 | coexpression of wild-type or mutant AGR2 with cysteine-rich regions of MUC2 |
5.3.4.1 | DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the enzyme fused to the human tissue plasminogen activator signal sequence in Escherichia coli strain DH10B |
5.3.4.1 | DNA and amino acid sequence determination and anaylsis, phylogenetic tree, expression in Escherichia coli as a soluble cytoplasmic protein |
5.3.4.1 | DNA and amino acid sequence determination, expression of His-tagged enzyme in Escherichia coli strain DH5alpha |