EC Number   |
|---|
   4.6.1.23 | - |
| 4.6.1.23 | alpha-sarcin cDNA cloned by consecutive annealing of overlapping primers followed by ligation into XhoI- and HindIII-sites in pcDNA3.1 or pcDNA3-mychis vector (C-terminal MycHis tag). alpha-Sarcin cDNA cloned into 3 × FLAG pMSCV to obtain a N-terminal 3 × Flag tagged version of alpha-sarcin. Expression of wild-type and mutant constructs in HeLa or Cos-7 cells |
| 4.6.1.23 | cloned and expressed in Escherichia coli |
| 4.6.1.23 | cloned and expressed in Escherichia coli BL21(DE3) |
| 4.6.1.23 | cloned and expressed in Escherichia coli RB791 |
| 4.6.1.23 | cloned and expressed in Escherichia coli TG2 |
| 4.6.1.23 | cloning of the genomic alpha-sarcin gene pGEM-T/FL-alpha plasmid, construction of the alpha-sarcin gene and its mutants by PCR, using genomic DNA as template |
| 4.6.1.23 | deletion mutant delta(7-22) produced in Escherichia coli |
| 4.6.1.23 | design of RNA fragment ECAS171 |
| 4.6.1.23 | doxycycline-inducible intracellular expression of wild-type alpha-sarcin in Saccharomyces cerevisiae strain W303 and in strains deficient for P1 or P2 protein, wild-type alpha-sarcin is lethal in all wild-type yeast clones, while 30% and 80% of resistant clones are obtained for the DELTAP1alphaP2beta and DELTAP2 strains, respectively |
