EC Number   |
|---|
   4.2.3.25 | - |
| 4.2.3.25 | cDNA from strawberry with plastid targeting and a synthetic intron is placed under the control of the wound inducible proteinase inhibitor 2 (PI2) promoter from potato. The construct is transformed to Arabidopsis thaliana ecotype Columbia 0 |
| 4.2.3.25 | cloning in a binary vector for introduction into Agrobacterium tumefaciens strain LBA4404 |
| 4.2.3.25 | Escherichia coli strain DH5alpha is used as the strain for cloning experiments and plasmid amplification. Saccharomyces cerevisiae wine strain T73-4 is used for functional expression of plasmids bearing the expression cassette. |
| 4.2.3.25 | expressed in Escherichia coli ArcticExpress (DE3) cells |
| 4.2.3.25 | expressed in Saccharomyces cerevisiae |
| 4.2.3.25 | functional expression in transgenic Petunia hybrida plants |
| 4.2.3.25 | gene LIS2, partial genomic library construction, DNA sequence determination and analysis, promoter sequence analysis, gene structure |
| 4.2.3.25 | His-tagged version expressed in Escherichia coli |
| 4.2.3.25 | into the pNZ8150 vector for expression in Lactococcus lactis |
