EC Number   |
|---|
    4.2.1.84 | - |
| 4.2.1.84 | chaperone GroEL2 is co-expressed with nitrile hydratase (NHase) in Escherichia coli in three ways: (a) monocistronic expression with one T7 promoter, (b) bicistronic expression with double T7 promoters, and (c) fusion expression with one T7 promoter driving the NHase-GroEL2 chimera. The NHase-GroEL2 chimera is the most successful expression strategy. Maximal nitrile hydratase activity is enhanced by 63.6% compared with the single NHase control |
| 4.2.1.84 | co-expression of recombinant strains YHJ-1 to YHJ-4 with plasmids encoding the chaperons pG-KJE8, pGro7, pKJE7, pG-Tf2 and pTf16 |
| 4.2.1.84 | Co-substituted NHase produced in Escherichia coli grown in Co supplemented medium |
| 4.2.1.84 | expressed in Escherichia coli BL21(DE3) cells |
| 4.2.1.84 | expression in Escherichia coli |
| 4.2.1.84 | expression in Escherichia coli BL21(DE3) |
| 4.2.1.84 | expression in Escherichia coli rosetta |
| 4.2.1.84 | expression in Escherichia coli, NovaBlue, series of recombinant plasmids with genes encoding NHase (nha1: alpha subunit and nha2: beta subunit) and P44k protein (nha3), different positions and combinations |
| 4.2.1.84 | expression in Escherichia coli, strain BL21(DE3) |
