EC Number   |
|---|
    3.3.2.10 | - |
| 3.3.2.10 | baculovirus expression system |
| 3.3.2.10 | cDNA extraction, RACE PCR with RNA-fragment of soluble epoxide hydrolase-like protein 1 (SPEH1), insertion into baculovirus transfection vector pACUW21 or pBacPAK8, recombinant baculoviruses generated by cotransfection of Spodoptera frugiperda Sf21 cells with described vector and Bsu361-cleaved BacPAK6 viral DNA, infection of Trichoplusia ni High Five cells |
| 3.3.2.10 | cDNA extraction, RACE PCR with RNA-fragment of soluble epoxide hydrolase-like protein 2 (SPEH2), insertion into baculovirus transfection vector pACUW21 or pBacPAK8, recombinant baculoviruses generated by cotransfection of Spodoptera frugiperda Sf21 cells with described vector and Bsu361-cleaved BacPAK6 viral DNA, infection of Trichoplusia ni High Five cells |
| 3.3.2.10 | cDNA library construction, DNA and amino acid sequence determination of the gene encoding the enzyme, genetic organization in a small EH gene family in soybean, overexpression in Escherichia coli strain BL21(DE3) |
| 3.3.2.10 | DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Spodoptera frugiperda Sf21 cells or in Trichoplusia ni Tn5 cells using the baculovirus infection system |
| 3.3.2.10 | DNA and amino acid sequence determination and analysis, expression of the potato enzyme in Spodoptera frugiperda cells and in Trichoplusia ni cells using the baculovirus infection system |
| 3.3.2.10 | DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, functional expression of wild-type enzyme and expression of enzyme mutants in Escherichia coli strain BL21(DE3) |
| 3.3.2.10 | enzyme expression in insect cells using the baculovirus infection system |
| 3.3.2.10 | expressed in CHO cells |
