EC Number   |
|---|
    3.2.1.55 | - |
| 3.2.1.55 | 2 genes, abf51A and abf62A, transposon mutagenesis |
| 3.2.1.55 | a histidine tag is introduced at the N-terminal end of AbfATK4, and the recombinant protein is expressed in Escherichia coli BL21, under control of isopropyl-beta-D-thiogalactopyranoside-inducible T7 promoter |
| 3.2.1.55 | ABF2 gene cloned and expressed in Saccharomyces cerevisiae |
| 3.2.1.55 | Abf62A-Axe6A is a modular enzyme comprising (in order from the N-terminus): an N-terminal signal peptide, a glycoside hydrolase family 62 (GH62) catalytic module, a family 6 carbohydrate binding module (CBM6), a dockerin module and an additional carbohydrate esterase family 6 catalytic module (CE6). Three Abf62A-Axe6A derivatives are constructed, overexpressed in Escherichia coli, purified, and biochemically characterized: 1. RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide, 2. RjAbf62A-CBM6, containing the GH62 and CBM6 modules, and 3. RjAxe6A, containing only CE6 |
| 3.2.1.55 | abfB gene, nucleotide sequence determined |
| 3.2.1.55 | alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae |
| 3.2.1.55 | alpha-L-arabinofuranosidase is expressed in combination with either the beta-glucosidases from Saccharomycopsis fibuligera or from Aspergillus kawachii in Saccharomyces cerevisiae strain VIN13 |
| 3.2.1.55 | arfI and arfII, genes encoding alpha-L-arabinofuranosidase |
| 3.2.1.55 | cDNA clones isolated |
