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Information on EC 3.2.1.55 - non-reducing end alpha-L-arabinofuranosidase
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EC Tree
3.2.1.55 non-reducing end alpha-L-arabinofuranosidaseIUBMB Comments
The enzyme acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans. Some beta-galactosidases (EC 3.2.1.23) and beta-D-fucosidases (EC 3.2.1.38) also hydrolyse alpha-L-arabinosides. cf. EC 3.2.1.185, non-reducing end beta-L-arabinofuranosidase.
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Word Map
- 3.2.1.55
- xylans
- arabinoxylans
- arabinose
- arabinans
- xylose
- arabinogalactans
- endoxylanase
- xylooligosaccharides
- spelt
-
hemicellulases
- xylanases
- xylobiose
-
arabinosylated
- nutrition
- biotechnology
-
pectic
- rhamnogalacturonans
- p-nitrophenyl-alpha-l-arabinofuranoside
-
xylanolytic
-
feruloylated
- l-arabitol
- paper production
-
arabinose-containing
-
debranched
- food industry
-
five-bladed
-
oat-spelts
-
cellulose-binding
-
hemicellulolytic
- kawachii
-
beechwood
- arabinofuranose
- arabino-oligosaccharides
- cellulosa
- arabinobiose
-
anti-complementary
- degradation
- p-nitrophenyl-beta-d-xylopyranoside
-
xylopyranosyls
- agriculture
-
xylan-degrading
-
exo-acting
- industry
- synthesis
- biofuel production
- drug development
- medicine
- analysis
The enzyme appears in viruses and cellular organisms
Synonyms
arabinofuranosidase, arabinosidase, alpha-l-araf, alpha-l-arabinosidase, alpha-arabinosidase, afase, alpha-l-arabinofuranosidase b, arabinoxylan arabinofuranohydrolase, alpha-araf, alpha-arabinofuranosidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ABF
Abf2
ABF3
Abf43A
AbF51
Abf51A
Abf62A
Abf62A-Axe6A
AbfA
AbfATK4
AbfB
AbfD3
AF
AF2
AFase
Afase I
Afase II
AFQ
AFQ1
AFS
AFS1
AfuB
AkabfA
AkAbfB
alpha-1,3-arabinofuranosidase
alpha-arabinofuranosidase B
alpha-AraF
alpha-L-Abfase
alpha-L-Afase
alpha-L-arabinofuranosidase
alpha-L-arabinofuranosidase B
alpha-L-arabinofuranoside arabinofuranohydrolase
alpha-L-arabinosidase
alpha-L-Araf
alpha-L-arafase
AN7908
arabinofuranosidase
arabinofuranosidase 3
bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities
arabinofuranosidase 43A
arabinoxylan alpha-L-1,3-arabinofuranohydrolase
arabinoxylan alpha-L-arabinofuranohydrolase
arabinoxylan arabinofuranohydrolase
arabinoxylan hydrolase
AraF
Araf-ase
AraF43
ARF
AvAbfB
AXH
AXH-d3
Axh43A
Axh43B
AXS5
Axy43A
beta-D-galactofuranosidase 4
beta-D-xylosidase/alpha-L-arabinofuranosidase
beta-D-xylosidase/alpha-L-arabinosidase
beta-xylosidase/alpha-arabinofuranosidase
beta-xylosidase/alpha-arabinosidase
ccxyl3a
Ct43Araf
CtGH43
exo-alpha-L-arabinofuranosidase
Galf-ase
GH 51 Abf
GH family 54 alpha-L-arabinofuranosidase
GH43 alpha-L-arabinofuranosidase
GH43B6
GH51 alpha-arabinofuranosidase
GH51 alpha-L-arabinofuranosidase
GH62
GH62 alpha-L-arabinofuranosidase
Hore_20580
L-arabinofuranosidase
L213A variant beta-glycosidase
ORF2812
ScAraf62A
STX-IV
Theth_1120
TmAFase
UmAbf62A
xarB
XylC
additional information
ABF3
bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities
alpha-L-arabinofuranoside arabinofuranohydrolase
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
beta-D-xylosidase/alpha-L-arabinofuranosidase
-
bifunctional enzyme displaying activity of EC 3.2.1.37 and EC 3.2.1.55
beta-xylosidase/alpha-arabinofuranosidase
bifunctional enzyme
beta-xylosidase/alpha-arabinofuranosidase
bifunctional enzyme
beta-xylosidase/alpha-arabinofuranosidase
bifunctional enzyme
ccxyl3a
-
gene name of multifunctional beta-xylosidase/alpha-L-arabinofuranosidase/beta-glucosidase
ccxyl3a
-
gene name of multifunctional beta-xylosidase/alpha-L-arabinofuranosidase/beta-glucosidase
additional information
enzyme belongs to the family 51 of glycosidases
additional information
enzyme belongs to the family 51 of glycosidases
additional information
-
the enzyme belongs to the glycosylhydrolase family GH93
additional information
-
enzyme belongs to glycosidase family 51
additional information
enzyme belongs to the family 51 of glycoside hydrolases
additional information
enzyme belongs to the family 51 of glycoside hydrolases
additional information
enzyme belongs to family 3 of glycoside hydrolases
additional information
-
the enzyme belongs to the glycosylhydrolase family GH54
additional information
enzyme belongs to family 51 of the glycosyl hydrolase classification as part of the 4/7 glycosyl hydrolase superfamily
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
-
-
-
-
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
the enzyme also acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans, some beta-galactosidases, EC 3.2.1.23, and beta-D-fucosidases, EC 3.2.1.38, also hydrolyse alpha-L-arabinosides
-
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
exo-acting enzyme
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
endolytic mode of action
-
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
catalytic mechanism, 2 stable states in the double displacement mechanism with formation of a transient covalent arabinofuranosyl-enzyme intermediate, structure of enzyme-substrate complex, substrate binding and specificity
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
catalytic mechanism, Glu175 is the acid-base residue, Glu294 functions as the catalytic nucleophile
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
enzyme shows 2 catalytic activities: alpha-L-arabinofuranosidase and beta-D-xylosidase activity
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
reaction mechanism, active site structure, Glu28, the catalytic nucleophil, Glu176, the acid-base residue, and Glu298, responsible for modulation of the ionization state of the acid-base and for substrate fixation, are important for catalytic activity
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
catalytic mechanism and substrate binding structure, overview
-
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
mode of action of Penicillium funiculosum family 62 isozymes, overview
-
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
the enzyme hydrolyze the glycosidic bond via the double-displacement mechanism in the active site of a funnel-shaped pocket, catalytic residues are the acid/base Glu172 and the enzymatic nucleophile Glu281
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
the enzyme also acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans, some beta-galactosidases, EC 3.2.1.23, and beta-D-fucosidases, EC 3.2.1.38, also hydrolyse alpha-L-arabinosides
-
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
exo-acting enzyme
-
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
endolytic mode of action
-
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
the enzyme hydrolyze the glycosidic bond via the double-displacement mechanism in the active site of a funnel-shaped pocket, catalytic residues are the acid/base Glu172 and the enzymatic nucleophile Glu281
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase
The enzyme acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans. Some beta-galactosidases (EC 3.2.1.23) and beta-D-fucosidases (EC 3.2.1.38) also hydrolyse alpha-L-arabinosides. cf. EC 3.2.1.185, non-reducing end beta-L-arabinofuranosidase.
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CAS REGISTRY NUMBER
COMMENTARY
9067-74-7
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1-5)-alpha-L-arabinofuranohexaose + H2O
alpha-L-arabinofuranose
-
-
-
?
1,5-alpha-L-arabinohexaose + H2O
1,5-alpha-L-arabinopentaose + L-arabinose
-
-
-
?
1,5-alpha-L-arabinotriose + H2O
1,5-alpha-L-arabinobiose + L-arabinose
-
-
-
?
2 p-nitrophenyl-alpha-L-arabinofuranoside
p-nitrophenol + p-nitrophenyl-alpha-L-arabinofuranosyl-(1,2)-alpha-L-arabinofuranoside
-
-
-
-
?
2 p-nitrophenyl-beta-D-xylopyranoside
p-nitrophenol + p-nitrophenyl-beta-D-xylopyranosyl-(1,2)-beta-D-xylopyranoside
-
-
-
-
?
2 p-nitrophenyl-beta-D-xylopyranoside
p-nitrophenol + p-nitrophenyl-beta-D-xylopyranosyl-(1,3)-beta-D-xylopyranoside
-
-
-
-
?
2',4',6'-trichlorophenyl alpha-L-arabinofuranoside + H2O
2,4,6-trichlorophenol + alpha-L-arabinofuranose
2',5'-dinitrophenyl alpha-L-arabinofuranoside + H2O
2,5-dinitrophenol + alpha-L-arabinofuranose
2(3),3(3)-di-alpha-L-arabinofuranosyl xylotriose + H2O
?
-
low activity
-
-
?
2(3)-alpha-L-arabinofuranosyl xylotriose + H2O
arabinose + xylotriose
-
high activity
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
-
1.7% of activity with p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
3',4'-dinitrophenyl alpha-L-arabinofuranoside + H2O
3',4'-dinitrophenol + alpha-L-arabinofuranose
-
-
-
r
3(2)-alpha-L-arabinofuranosyl xylobiose + H2O
?
-
high activity
-
-
?
3(3)-alpha-L-arabinofuranosyl xylotetraose + H2O
arabinose + xylotetraose
-
high activity
-
-
?
3-nitrophenyl-alpha-L-arabinofuranoside + H2O
3-nitrophenol + alpha-L-arabinofuranose
-
-
-
r
3-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
alpha-L-arabinofuranose
-
-
-
-
?
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-chloro-2-nitrophenol + L-arabinofuranose
4-methylumbelliferyl alpha-L-arabinofuranoside + H2O
4-methylumbelliferol + alpha-L-arabinofuranose
4-methylumbelliferyl-alpha-L-arabinopyranoside + H2O
4-methylumbelliferol + alpha-L-arabinopyranose
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
isoform AbfII exhibits very low activity with this substrate
-
-
?
4-nitrophenyl alpha-D-glucoside + H2O
4-nitrophenol + D-glucose
-
9% of the rate exhibited on p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
?
-
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl beta-D-glucofuranoside + H2O
4-nitrophenol + beta-D-glucofuranose
-
very low activity
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
low activity
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
-
isoform AbfII exhibits very low activity with this substrate
-
-
?
4-nitrophenyl beta-D-mannoside + H2O
4-nitrophenol + D-mannose
-
3.4% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-xyloside + H2O
4-nitrophenol + D-xylose
-
100% activity
-
-
?
4-nitrophenyl beta-L-fucopyranoside + H2O
4-nitrophenol + beta-L-fucopyranose
-
very low activity
-
-
?
4-nitrophenyl beta-L-galactofuranoside + H2O
4-nitrophenol + beta-L-galactofuranose
-
very low activity
-
-
?
4-nitrophenyl beta-L-xyloside + H2O
4-nitrophenol + beta-L-xylose
-
little activity
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenyl + L-arabinopyranose
-
-
-
?
alkyl-alpha-arabinofuranoside + H2O
L-arabinose + alkyl alcohol
-
-
-
-
?
alpha-(1->5)-L-arabinofuranotriose + H2O
?
branched and unbranched, feruloylation of branched substrate strongly decreases the enzyme activity of isozyme rAFQ1
-
-
?
alpha-(1->5)-L-arabinofuranotriose + H2O
alpha-L-arabinofuranose
-
-
-
?
alpha-1,5-L-arabinohexaose + H2O
alpha-1,5-L-arabinobiose + alpha-1,5-L-arabinotetraose
-
-
-
-
?
alpha-1,5-L-arabinopentaose + H2O
alpha-1,5-L-arabinobiose + alpha-1,5-L-arabinotriose
-
-
-
-
?
alpha-1,5-L-arabinotriose + H2O
alpha-1,5-L-arabinobiose + alpha-L-arabinose
-
-
-
-
?
alpha-L-arabinan + H2O
oligo-alpha-1,5-L-arabinose + ?
-
-
the enzyme releases alpha-1,5-L-arabinobiose from the nonreducing end of the polysaccharide
-
?
alpha-L-arabinofuranoptetraose + H2O
alpha-L-arabinofuranose
-
-
-
?
alpha-L-arabinofuranosyl 1-naphthol + H2O
alpha-L-arabinofuranose + 1-naphthol
-
-
-
-
?
alpha-L-arabinofuranosyl 3,4-dinitrophenol + H2O
alpha-L-arabinofuranose + 3,4-dinitrophenol
-
-
-
-
?
alpha-L-arabinofuranosyl 3,5-dinitrophenol + H2O
alpha-L-arabinofuranose + 3,5-dinitrophenol
-
-
-
-
?
alpha-L-arabinofuranosyl 4-bromoisoquinoline + H2O
alpha-L-arabinofuranose + 4-bromoisoquinoline
-
-
-
-
?
alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O
methylumbelliferone + alpha-L-arabinofuranose
alpha-L-arabinofuranosyl 4-phenylphenol + H2O
alpha-L-arabinofuranose + 4-phenylphenol
-
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranose + 2 H2O
2 L-arabinofuranose + D-xylopyranose
-
-
complete hydrolysis
-
?
alpha-L-arabinofuranosyl-1,4-alpha-D-xylopyranoside + H2O
L-arabinofuranose + D-xylopyranose
-
-
-
-
?
arabinan + H2O
alpha-L-arabinofuranose
-
from sugar beet, not from wheat
-
-
?
arabinan + H2O
L-arabinofuranose + ?
release of L-arabinofuranose
-
-
?
arabinoxylan + H2O
alpha-L-arabinofuranose + ?
-
soluble or insoluble
-
-
?
arabinoxylan + H2O
alpha-L-arabinofurnanose + ?
when N-terminus (N_term, GH43, and CBM22) and C-terminus (C_term, GH43, and CBM6) of XynF are cloned and expressed separately. Neither segment of XynF is active on xylan, although both of the separate domains hydrolyze 4-nitrophenyl arabinose and arabinoxylan (wheat and rye)releasing alpha-L-arabinofurnanose
-
-
?
barley arabinoxylan + H2O
L-arabinose + ?
-
on barley arabinoxylan-0.76 the combination of isoform AbfA and AXH-d3 cleaves 35% of the total arabinose
-
-
?
beta-D-Galp-(1->6)-[alpha-L-Araf-(1->3)]-beta-D-Galp-(1->6)-beta-D-Galp + H2O
L-arabinofuranose + beta-D-Gal-(1->6)-beta-D-Gal-(1->6)-beta-D-Gal
recombinant BlArafA releases L-arabinose from the substrate, which is a beta-1,6-galactan side chain containing alpha-1,3-linked L-Araf
-
-
?
ginsenoside Mc + H2O
ginsenoside K + L-arabinofuranose
-
-
-
?
ginsenoside Rd + H2O
ginsenoside K + D-glucopyranose
-
-
-
?
gum arabic + H2O
L-arabinofuranose + ?
release of L-arabinofuranose, low activity
-
-
?
larch arabinoxylan + H2O
?
substrate of mutant F435Y/Y446F, no activity with the wild-type enzyme
-
-
?
larch type II arabinogalactan + H2O
L-arabinofuranose + ?
release of L-arabinofuranose, degradation profiles of type II AG
-
-
?
methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
methyl alpha-L-arabinofuranoside + L-arabinofuranose
-
-
-
-
?
methyl alpha-L-Araf-(1->2)-alpha-L-Araf + H2O
L-arabinofuranose + L-Araf-alpha-O-methyl ester
low activity compared to methyl alpha-L-Araf-(1->3)-alpha-L-Araf
-
-
?
methyl alpha-L-Araf-(1->3)-alpha-L-Araf + H2O
L-arabinofuranose + L-Araf-alpha-O-methyl ester
-
-
-
?
methyl alpha-L-Araf-(1->5)-alpha-L-Araf + H2O
L-arabinofuranose + L-Araf-alpha-O-methyl ester
low activity compared to methyl alpha-L-Araf-(1->3)-alpha-L-Araf
-
-
?
O-alpha-L-arabinofuranosyl-(1-3-)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
L-arabinose + O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
O-beta-D-xylopyranosyl-(1-4)-[O-alpha-L-arabinofuranosyl-(1-3)]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
L-arabinose + O-beta-D-xylopyranosyl-(1-4)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
o-nitrophenyl-beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
3% of activity with p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl alpha-L-arabinofuranoside + benzyl-alpha-D-xylopyranoside
?
-
-
-
-
?
p-nitrophenyl-alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactopyranose
6.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
p-nitrophenol + alpha-D-glucopyranose
5.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl-alpha-L-arabinopyranoside + H2O
p-nitrophenol + L-arabinopyranose
0.4% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + beta-D-fucopyranose
8.3% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl-beta-D-galactofuranoside
beta-D-galactofuranosyl-(1,2)-beta-D-galactofuranoside + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
0.33% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
p-nitrophenol + beta-D-galactopyranose
19% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
p-nitrophenol + ?
9.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl-beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannopyranose
2.8% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
p-nitrophenyl-beta-D-xylofuranoside + H2O
p-nitrophenol + beta-D-xylofuranose
-
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + ?
0.9% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
phenyl-alpha-L-arabinofuranoside + H2O
phenol + L-arabinofuranose
-
-
-
-
?
polygalacturonase-soluble carbohydrates + H2O
?
-
PGSC of alfalfa cell walls
-
-
?
radish root arabinogalactan-protein + H2O
L-arabinofuranose + ?
release of L-arabinofuranose
-
-
?
rye arabinoxylan + H2O
L-arabinose
-
on rye arabinoxylan-0.52 the combination of isoform AbfA and AXH-d3 cleaves 30% of the total arabinose
-
-
?
softwood Kraft pulp + H2O
L-arabinose + ?
-
arabinoglucuronoxlan I and II
-
-
?
sugar beet arabinan + H2O
alpha-L-arabinofuranose + ?
-
-
-
?
sugar beet arabinan + H2O
arabinose + ?
-
0.82% L-arabinose liberated after 72 h incubation at 28°C
-
?
sugar beet arabinan + H2O
L-arabinofuranose + ?
-
-
release of 24.5% of L-arabinofuranose
-
?
sugar beet debranched arabinan + H2O
alpha-L-arabinofuranose + ?
-
-
-
?
wheat flour arabinoxylan + H2O
L-arabinofuranose + ?
-
-
release of 23% of L-arabinofuranose
-
?
wheat flour arabinoxylan + H2O
L-arabinose + ?
-
15.9% L-arabinose liberated after 72 h incubation at 28°C
-
?
(1-5)-alpha-L-arabinofuranooligosaccharides + H2O
alpha-L-arabinose + ?
-
-
-
-
?
(1-5)-alpha-L-arabinofuranooligosaccharides + H2O
alpha-L-arabinose + ?
-
-
-
-
?
1,5-alpha-L-arabinofuranohexaose + H2O
L-arabinose + L-arabinopentaose
-
-
-
-
?
1,5-alpha-L-arabinofuranohexaose + H2O
L-arabinose + L-arabinopentaose
-
-
-
?
1,5-alpha-L-arabinofuranohexaose + H2O
L-arabinose + L-arabinopentaose
-
-
-
-
?
1,5-alpha-L-arabinofuranohexaose + H2O
L-arabinose + L-arabinopentaose
-
-
-
-
?
1,5-alpha-L-arabinofuranohexaose + H2O
L-arabinose + L-arabinopentaose
-
-
-
-
?
1,5-alpha-L-arabinotetraose + H2O
L-arabinose
-
-
-
?
1,5-alpha-L-arabinotriose + H2O
L-arabinose
-
-
-
?
1,5-L-arabinan + H2O
L-arabinose + ?
-
-
-
-
ir
2',4',6'-trichlorophenyl alpha-L-arabinofuranoside + H2O
2,4,6-trichlorophenol + alpha-L-arabinofuranose
-
-
-
r
2',4',6'-trichlorophenyl alpha-L-arabinofuranoside + H2O
2,4,6-trichlorophenol + alpha-L-arabinofuranose
-
-
-
r
2',5'-dinitrophenyl alpha-L-arabinofuranoside + H2O
2,5-dinitrophenol + alpha-L-arabinofuranose
-
-
-
r
2',5'-dinitrophenyl alpha-L-arabinofuranoside + H2O
2,5-dinitrophenol + alpha-L-arabinofuranose
-
-
-
r
2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2,5-dinitrophenyl beta-D-xylopyranoside + H2O
2,5-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
2,5-dinitrophenyl beta-D-xylopyranoside + H2O
2,5-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O
2,5-dinitrophenol + L-arabinofuranose
-
-
-
-
2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O
2,5-dinitrophenol + L-arabinofuranose
-
-
-
-
?
2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O
2,5-dinitrophenol + L-arabinofuranose
-
-
-
-
?
2-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2-alpha-L-arabinofuranosyl xylotriose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
2-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
2-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
alpha-L-arabinofuranose
-
-
-
-
?
2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
alpha-L-arabinofuranose
-
-
-
-
?
23,33-di-alpha-L-arabinofuranosylxylotriose + H2O
arabinose + ?
-
-
-
?
23,33-di-alpha-L-arabinofuranosylxylotriose + H2O
arabinose + ?
-
-
-
?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
3,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
3,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
3-alpha-L-arabinofuranosyl xylobiose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
3-alpha-L-arabinofuranosyl xylobiose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
3-alpha-L-arabinofuranosyl xylobiose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
3-alpha-L-arabinofuranosyl xylobiose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
3-alpha-L-arabinofuranosyl xylobiose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
3-alpha-L-arabinofuranosyl xylobiose + H2O
alpha-L-arabinose + ?
-
-
-
-
?
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-chloro-2-nitrophenol + L-arabinofuranose
-
-
-
-
?
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-chloro-2-nitrophenol + L-arabinofuranose
-
-
-
-
?
4-methylumbelliferyl alpha-L-arabinofuranoside + H2O
4-methylumbelliferol + alpha-L-arabinofuranose
-
-
-
?
4-methylumbelliferyl alpha-L-arabinofuranoside + H2O
4-methylumbelliferol + alpha-L-arabinofuranose
-
-
-
?
4-methylumbelliferyl-alpha-L-arabinopyranoside + H2O
4-methylumbelliferol + alpha-L-arabinopyranose
-
artificial
-
-
?
4-methylumbelliferyl-alpha-L-arabinopyranoside + H2O
4-methylumbelliferol + alpha-L-arabinopyranose
-
artificial
-
-
?
4-nitrophenyl + H2O
4-nitrophenol + beta-D-xylopyranose
AM283040
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
100% activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
Aspergillus nidulans A773
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
100% activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
100% activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
r
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
r
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
Geobacillus vulcani GS90
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
best substrate
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
low alpha-L-arabinofuranosidase activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
low alpha-L-arabinofuranosidase activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
high activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
high activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
hydrolysis of the substrate with retention of the anomeric configuration
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
100% activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
best substrate
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
best substrate
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
14.4% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
also synthesis of regioisomers of di- to pentaarabinofuranosides
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
GH51 alpha-L-arabinofuranosidase
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
AM283040
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
AM283040
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
Streptomyces chartreusis JHA19
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
Streptomyces sp. JHA19 NRRL F-5140
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
the substrate binding process is driven by favourable entropy, which is linked to the movement of the beta2alpha2 loop. Loop closure relocates the solvent-exposed W99 into a buried location, allowing its involvement in substrate binding and in the formation of a functional active site. Residue H98 has a role in the dynamic formation as catalytically operational active site, which may be a specific feature of a subset of GH51 arabinofuranosidases
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinose
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
very low activity
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
Lactobacillus brevis DSM 1269
-
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
Weissella sp. 142
-
-
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
4-nitrophenol + beta-D-galactofuranose
AM283040
-
-
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
4-nitrophenol + beta-D-galactofuranose
AM283040
-
-
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
4-nitrophenol + beta-D-galactofuranose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
very low activity
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
16% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
9% of activity with p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
0.26% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside
4-nitrophenol + D-xylopyranose
AM283040
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside
4-nitrophenol + D-xylopyranose
AM283040
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
isoform AbfII exhibits very low activity with this substrate
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
substrate for isoform AbfA
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
20% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
low activity only of enzyme AFS1, not AFQ1
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
low activity only of enzyme AFS1, not AFQ1
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
52% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-L-glucoside + H2O
4-nitrophenol + beta-L-glucose
-
little activity
-
-
?
4-nitrophenyl beta-L-glucoside + H2O
4-nitrophenol + beta-L-glucose
-
little activity
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
low activity
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
low activity
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
11% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
alpha-L-arabinofuranose
-
-
-
?
5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
alpha-L-arabinofuranose
-
-
-
-
?
alpha-1,5-L-arabinopentaose + H2O
L-arabinose + ?
low activity
-
-
?
alpha-1,5-L-arabinopentaose + H2O
L-arabinose + ?
low activity
-
-
?
alpha-1,5-L-arabinotetraose + H2O
L-arabinose + ?
low activity
-
-
?
alpha-1,5-L-arabinotetraose + H2O
L-arabinose + ?
low activity
-
-
?
alpha-1,5-L-arabinotriose + H2O
L-arabinose + ?
low activity
-
-
?
alpha-1,5-L-arabinotriose + H2O
L-arabinose + ?
low activity
-
-
?
alpha-1,5-linked arabinoheptaose + H2O
L-arabinose + ?
-
-
-
?
alpha-L-arabinan + H2O
alpha-1,5-L-arabinobiose + ?
-
the enzyme releases alpha-1,5-L-arabinobiose from the nonreducing end of the polysaccharide
-
?
alpha-L-arabinan + H2O
alpha-1,5-L-arabinobiose + ?
-
the enzyme is highly active and displays a strict substrate specificity for linear alpha-1,5-L-arabinan
the enzyme releases alpha-1,5-L-arabinobiose from the nonreducing end of the polysaccharide
-
?
alpha-L-arabinofuranobiose + H2O
alpha-L-arabinofuranose
-
-
-
?
alpha-L-arabinofuranobiose + H2O
alpha-L-arabinofuranose
-
-
-
?
alpha-L-arabinofuranopentaose + H2O
alpha-L-arabinofuranose
-
-
-
?
alpha-L-arabinofuranopentaose + H2O
alpha-L-arabinofuranose
-
-
-
?
alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O
methylumbelliferone + alpha-L-arabinofuranose
-
-
-
-
?
alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O
methylumbelliferone + alpha-L-arabinofuranose
-
-
-
-
?
alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O
methylumbelliferone + alpha-L-arabinofuranose
-
-
-
-
?
alpha-L-Araf-(1->3)-[alpha-L-Araf-(1->5)]-alpha-L-Araf + H2O
?
branched, the activity of isozyme rAFS1on feruloylated branched L-arabinofuranotriose is almost the same as its activity on branched substrate
-
-
?
alpha-L-Araf-(1->3)-[alpha-L-Araf-(1->5)]-alpha-L-Araf + H2O
?
branched, the activity of isozyme rAFS1on feruloylated branched L-arabinofuranotriose is almost the same as its activity on branched substrate
-
-
?
arabinan + H2O
alpha-L-arabinose + ?
-
from sugar beet, enzyme shows preference for hydrolysis of the terminal arabinofuranosyl residues, (1-5)-linked, of the arabinan backbone rather than the arabinosylside chain, alpha(1-3)-linked residues
-
-
?
arabinan + H2O
alpha-L-arabinose + ?
-
from sugar beet, enzyme shows preference for hydrolysis of the terminal arabinofuranosyl residues, (1-5)-linked, of the arabinan backbone rather than the arabinosylside chain, alpha(1-3)-linked residues
-
-
?
arabinan + H2O
L-arabinose + ?
-
XYL3 XYL3 is able to degrade all terminal arabinosyl residues and participates in the in-vivo hydrolysis of arabinan
-
-
?
arabinoxylan + H2O
?
the arabinose-binding domain of AkAbf54 is named AkCBM42. In the complex between AkAbf54 and arabinofuranosyl-alpha-1,2-xylobiose, the arabinose moiety occupies the binding pocket of AkCBM42, whereas the xylobiose moiety is exposed to the solvent. AkCBM42 facilitates the hydrolysis of insoluble arabinoxylan, because mutants at the arabinose binding site exhibit markedly decreased activity. AkCBM42 interacts with arabinose-substituted, but not with unsubstituted, hemicelluloses
-
-
?
arabinoxylan + H2O
?
the enzyme releases arabinose from maize fiber, oat spelt arabinoxylan, and wheat arabinoxylan, but not from larch wood arabinogalactan or alpha-1,5-debranched arabinan
-
-
?
arabinoxylan + H2O
?
the enzyme releases arabinose from maize fiber, oat spelt arabinoxylan, and wheat arabinoxylan, but not from larch wood arabinogalactan or alpha-1,5-debranched arabinan
-
-
?
arabinoxylan + H2O
?
-
GH43 enzyme selectively hydrolyses (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides. Combinations (50:50) of the GH43 alpha-AraF from Bifidobacterium sp. and the GH51 alpha-AraFs from Meripilus giganteus or Humicola insolens results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan
-
-
?
arabinoxylan + H2O
?
-
GH43 enzyme selectively hydrolyses (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus or Humicola insolens results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C
-
-
?
arabinoxylan + H2O
?
-
GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from singly substituted xylopyranosyls in arabinoxylan. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C
-
-
?
arabinoxylan + H2O
?
-
GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from single substituted xylopyranosyls in arabinoxylan. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C
-
-
?
arabinoxylo-oligosaccharides + H2O
?
-
GH43 enzyme selectively hydrolysed (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides
-
-
?
arabinoxylo-oligosaccharides + H2O
?
-
GH43 enzyme selectively hydrolysed (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosacchari