EC Number   |
|---|
   3.1.3.74 | cDNA, from brain, expression in Escherichia coli M15/pRER4, sequencing, ORF is located on chromosome 22q12.3, genomic organization |
| 3.1.3.74 | cloned into plasmid pET15b, expression in Escherichi coli BL21 (DE3) as His-tag fusion protein |
| 3.1.3.74 | expression in Escherichia coli HMS174(DE3) |
| 3.1.3.74 | full-length cDNA, from brain, sequencing, ORF is located on chromosome 15.E1, genomic organization |
| 3.1.3.74 | getting Tat-PLPP/CIN: amplification of PLPP/CIN via PCR and inserted into pGEM-T vector, subcloned into pET15b, transformation of BL21 Escherichia coli. getting PLPP: cDNA of PLPP amplified via PCR and cloned into pET15b, transformation of Escherichia coli JM109 (DE3). experiments with Sprague-Dawley (SD) rats (7 weeks old). The effect of PNP treatment on PLPP/CIN immunoreactivity in the rat hippocampus: vitamin B6 may not be directly involved in ADF/cofilin-mediated F-actin depolymerization in normal condition. Up-regulation of PLPP/CIN expression following status epilepticus may play an important role in the formation of epileptic hippocampus. Hippocampal excitability following transduction of Tat-PLPP/CIN: F-actin depolymerization induced by over-expression of PLPP/CIN may increase excitability ratio in the hippocampus via postsynaptic changes, not via impaired GABAergic inhibition (immunohistochemistry, optical density, enzyme activity, electrophysiological analysis) |
| 3.1.3.74 | getting Tat-PLPP/CIN: amplification of PLPP/CIN via PCR and inserted into pGEM-T vector, subcloned into pET15b, transformation of BL21 Escherichia coli. getting PLPP: cDNA of PLPP amplified via PCR and cloned into pET15b, transformation of Escherichia coli JM109 (DE3). investigation whether expression of PLPP/CIN is altered following long-term potentiation (LTP) induction and whether Tat-PLPP/CIN transduction affects LTP induction in the rat Sprague-Dawley. PLPP/CIN immunoreactivity is decreased in dentate granule cells after induction of LTP (induction: stimulating electrode for high frequency stimulation and recording electrode). Tat-PLPP/CIN transduction (20 and 200 microg/kg) decreased the efficiency of high frequency stimulus-induced potentiation of populations spike amplitude as compared to saline or Tat-protein-treated animals. PLPP/CIN protein level shows inverse correlation with phosphorylated ADF/cofilin levels and F-actin content (immunohistochemistry). conclusion: PLPP/CIN-mediated actin dynamics may play important role in changes of morphological properties (dendritic spine reorganization) of the hippocampus in LTP. |
| 3.1.3.74 | His-tag fusion, expression in Escherichia coli |
| 3.1.3.74 | into pCRII-TOPO vector, 0.86 kb SmaI fragment from TOPOpdxP ligated into the SmaI site of pKK223-3, overexpression in Escherichia coli JM109. Overexpression in Sinorhizobium meliloti IFO 14782 recombinant with pVKPtacpdxP |
| 3.1.3.74 | into pET15b vector and expressed in Escherichia coli BL21(DE3) |
| 3.1.3.74 | PLPP gene fused with a PEP-1 peptide (PEP-1-PLPP) and 6His-tag and cloned into pET-15b. Expression in Escherichia coli BL21 (DE3) transduced into PC12 cells at various times and concentrations to evaluate the transduction ability (analyzed by Western blotting, fluorescence labeled). PLP concentration in cells PC12 cells changed by transduced PEP-1-PLPP fusion protein (spectroscopic measurement) |
