EC Number   |
|---|
    3.1.1.14 | Chlase gene, DNA and amino acid sequence determination and anaylsis, expression of precursor full-length wild-type Chlase and of a mutant ChlaseDELTAN lacking the N-terminal 21 amino acids, which corresponds to the mature enzyme, in two heterologous plant systems: in Cucurbita pepo cv. Maยayan plants using a ZYMV-based viral vector infective clone system and inoculation of cotyledons, and transiently in Nicotiana tabacum cv. Samsun NN chloroplast membranes of protoplasts, expression of full-length and truncated enzyme versions as thioredoxin fusion proteins in Escherichia coli |
| 3.1.1.14 | DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, and phylogenetic analysis and tree, the isozymes from cultivars TN57, CN1927, and CYY8467 share 95% identity, and an eight-nucleotide sequence of Chlase in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation |
| 3.1.1.14 | DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, and phylogenetic analysis and tree, the isozymes from cultivars TN57, CN1927, and CYY8467 share 95% identity, and an eight-nucleotide sequence of Chlase2 in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation implying that a frame shift mutation of the Chlase gene has evolved in CYY8467 |
| 3.1.1.14 | expressed in Escherichia coli |
| 3.1.1.14 | expressed in Escherichia coli BL21 (DE3) cells |
| 3.1.1.14 | expressed in Escherichia coli BL21 cells |
| 3.1.1.14 | expressed in Escherichia coli BL21(DE3) cells |
| 3.1.1.14 | expressed in Escherichia coli Rosseta-Gami B (DE3) cells |
| 3.1.1.14 | expressed in Escherichia coli strain BL21(DE3) |
| 3.1.1.14 | expression as His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
