EC Number   |
Reference  |
|---|
    2.7.7.43 | transformation of the CMP-NeuNAc defective Escherichia coli K1 strain EV5 with CMP-NeuNAc synthetase from Neisseria meningitidis can complement the defect in Escherichia coli |
645259 |
| 2.7.7.43 | high-level expression in Escherichia coli |
645266 |
| 2.7.7.43 | expression in Escherichia coli, enzyme can function in K1 capsular polysaccharide biosynthesis in Escherichia coli |
645271 |
| 2.7.7.43 | cloning is achieved by complementation of the Chineses hamster ovary lec32 mutation that causes a deficiency in CMP-N-acetylneuraminate synthetase activity, it also causes polysialic acid to be expressed in the capsule of the CMP-Neu5Ac synthetase negative Escherichia coli mutant EV5 |
645273 |
| 2.7.7.43 | expression in CHO cells or Escherichia coli |
645273 |
| 2.7.7.43 | the gene is cloned into a T7 expression vector, the protein is expressed in Escherichia coli |
645274 |
| 2.7.7.43 | the enzyme is subcloned for overexpression in Escherichia coli K12 using the expression vector pKK233-3 |
645279 |
| 2.7.7.43 | a series of deletions from the 3'-end of the CMP-NeuAc synthetase coding region is constructed and expressed in Escherichia coli |
662202 |
| 2.7.7.43 | cloned in Escherichia coli XL-10 |
671567 |
| 2.7.7.43 | expressed in Escherichia coli strain BL21 |
671567 |
