EC Number |
---|
2.7.7.12 | - |
2.7.7.12 | 3 to 4fold overexpression in strain BL21(DE3) |
2.7.7.12 | cloning in Escherichia coli and coexpression in yeast of mutant heterodimers derived from galactosemic patients, use of different tags |
2.7.7.12 | construction and overexpression of bifunctional fusion protein composed of galactose-2-phosphate uridylyltransferase and UDP-galactose 4-epimerase with an intervening linker of 3 Ala residues |
2.7.7.12 | expression as fusion protein with glutathione-S-transferase from Schistosoma japonicum in a bacterial expression system |
2.7.7.12 | expression in Escherichia coli |
2.7.7.12 | expression in Escherichia coli BL21 |
2.7.7.12 | expression in Sacchaormyces cerevisiae |
2.7.7.12 | expression of His- and 12CA5 epitope-tagged subunits of mutants R333W and Q188R in yeast, analysis of homo- and heterodimer formation of wild-type and mutant subunits |
2.7.7.12 | expression of mutant H166G in strain CA13, expression of mutant H166A in strain BL21(DE3)pLysS |