EC Number   |
|---|
    2.7.2.4 | - |
| 2.7.2.4 | 2 subunits, alpha and beta, encoded by an inframe overlapping gene, askAB genes cloned and expressed in Escherichia coli GT3 |
| 2.7.2.4 | ask gene conferred to Escherichia coli DH5alpha transformants |
| 2.7.2.4 | ask-asd operon cloned |
| 2.7.2.4 | aspartokinase encoded by lysC, expressed in Escherichia coli HB101 |
| 2.7.2.4 | aspartokinase gene (ask) by cloning into an Escherichia coli/Corynebacterium glutamicum shuttle expression vector. Recombinant vector is transformed into Escherichia coli DH5alpha and then into Corynebacterium glutamicum. The induction of recombinant vector by IPTG has an inhibitory effect on cell growth due to over-expression of the cloned gene. A 2fold increase in lysine production is observed by cloning of the ASK gene in Corynebacterium glutamicum rather than in Escherichia coli, due to the presence of lysine exporter channel which facilitates lysine extraction |
| 2.7.2.4 | both catalytic domains of the enzyme, performing each one of the enzyme activities, are expressed separately with or without the interface region, resulting in increased activity of each domain compared to the wild-type bifunctional holoenzyme, the isolated catalytic domains are no longer allosterically regulated, expression of hybrid holoenzyme AKIII-HDHI+ |
| 2.7.2.4 | by functional complementation of a Saccharomyces cerevisiae strain mutated in its homoserine dehydrogenase gene (hom6), expression in Escherichia coli, two of the three isolated clones are also able to complement a mutant yeast aspartate kinase gene (hom3), expression of the AK-HSDH gene in Arabidopsis thaliana (meristematic cells, leaves and stamens) |
| 2.7.2.4 | cDNA located on chromosome 4, cloned in Escherichia coli DH5alpha, overproduced in Escherichia coli BL21pLysS |
| 2.7.2.4 | DR1365 gene of Deinococcus radiodurans is isolated, after PCR amplification and several steps the amplified DNA fragment is introduced into Deinococcus radiodurans ATCC13939 to generate a DR1365 disruptant. |
